Effect of Bakuchiol on Leukocyte Functions and Some Inflammatory Responses in Mice

Abstract

The effects of bakuchiol, a meroterpenoid isolated from the leaves of Psoralea glandulosa L., on phospholipase A2 (PLA2) activity from different sources, human neutrophil responses, zymosan air pouch and topical inflammation in mice, were investigated. This natural product was a weak inhibitor of secretory and intracellular PLA2 but dose-dependently reduced the formation of LTB4 and TXB2 by human neutrophils and platelet microsomes, respectively. In addition, bakuchiol inhibited degranulation in human neutrophils, whereas superoxide generation was not affected. In mice, bakuchiol decreased cell migration, myeloperoxidase activity and eicosanoid levels in the air pouch inflammation induced by zymosan. After topical administration, this compound was effective as an inhibitor of oedema and myeloperoxidase activity in the 12-O-tetradecanoylphorbol 13-acetate (TPA)-induced ear oedema and significantly reduced the PGE2 content and ear oedema in the arachidonic acid-induced response. Bakuchiol is a natural anti-inflammatory agent able to control leukocytic functions such as eicosanoid production, migration and degranulation in the inflammatory site.

Inhibition of 5-Lipoxygenase Product Synthesis by Natural Compounds of Plant Origin

“…Interestingly, 5-LO and cPLA 2 share structural (C2 domain) and regulatory properties [activated by Ca 2+ and by phosphorylation by members of the mitogen-activated protein kinase (MAPK) family] [31], [34]. Indeed, many studies revealed that natural compounds (e. g., flavonoids and other polyphenols) leading to suppression of LT formation also act as PLA 2 inhibitors preventing AA release [35], [36], [37], [38], [39], [40]. The anti-inflammatory sesterterpenoid manoalide, for instance, is an irreversible inhibitor of PLA 2 enzymes by covalently modifying lysine residues [41].…”

Analgesic, anti-inflammatory, anticonvulsant and CNS effect of the ethanolic extract of Butea monosperma roots

“…Croton oil contains TPA as main irritant agents which is able to activate protein kinase C (PKC), which activates other enzymatic cascades in turn, leading to release of platelet activation factor (PAF) and AA. This cascade of events stimulates vascular permeability, vasodilation, polymorphonuclear leukocytes migration, release of histamine and serotonin and moderate synthesis of inflammatory eicosanoids by cyclooxygenase (COX) and 5-lipoxygenase (5-LOX) enzymes (Ferrandiz et al, 1996;Wang et al, 2001). As shown in able 5, ethanolic extract of BM (800 mg/kg, p.o.)…”

Hepatoprotective Effect of Calotropis procera in Isoniazid and Rifampicin Induced Hepatotoxicity

“…41 Degranulation of neutrophils in plasma membrane by modulating Ca 2+ channels ban be hampered by fl avonoids. 42 Various studies proved the successful use of rat's models for INH and RMP induced hepatotoxicity. 11,43 Therefore, same model has been used to determine the hepatoprotective activity of CP in the anti-TB drug-induced toxicity.…”