Effect of ATP and its analogs on contractility of rat myocardium during ontogeny

Anikina, T. A.; Bilalova, G. A.; Zverev, A. A.; Ситдиков, Ф. Г.

doi:10.1007/s10517-007-0239-z

Cited by 4 publications

(2 citation statements)

References 6 publications

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“…The inhibitory effects produced by ATP and β-NAD were weakly age-dependent and were almost similar at all studied stages of postnatal ontogenesis in all types of myocardial preparations except adult ventricular myocardium. Several studies showed that extracellular ATP affects cardiac function in juvenile and adult mammals via purine P1 and P2 receptors [23]. Here, we show that β-NAD induces direct postsynaptic nerve-independent cardiac effects in early postnatal life similarly to ATP.…”

Section: Age-specific Effects Of Atp and β-Nad In The Rat Heartsupporting

confidence: 50%

Extracellular ATP and β-NAD alter electrical properties and cholinergic effects in the rat heart in age-specific manner

Pustovit

Потехина

Ivanova

et al. 2019

Purinergic Signalling

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Extracellular ATP and nicotinamide adenine dinucleotide (β-NAD) demonstrate properties of neurotransmitters and neuromodulators in peripheral and central nervous system. It has been shown previously that ATP and β-NAD affect cardiac functioning in adult mammals. Nevertheless, the modulation of cardiac activity by purine compounds in the early postnatal development is still not elucidated. Also, the potential influence of ATP and β-NAD on cholinergic neurotransmission in the heart has not been investigated previously. Age-dependence of electrophysiological effects produced by extracellular ATP and β-NAD was studied in the rat myocardium using sharp microelectrode technique. ATP and β-NAD could affect ventricular and supraventricular myocardium independent from autonomic influences. Both purines induced reduction of action potentials (APs) duration in tissue preparations of atrial, ventricular myocardium, and myocardial sleeves of pulmonary veins from early postnatal rats similarly to myocardium of adult animals. Both purine compounds demonstrated weak age-dependence of the effect. We have estimated the ability of ATP and β-NAD to alter cholinergic effects in the heart. Both purines suppressed inhibitory effects produced by stimulation of intracardiac parasympathetic nerve in right atria from adult animals, but not in preparations from neonates. Also, ATP and β-NAD suppressed rest and evoked release of acetylcholine (ACh) in adult animals. β-NAD suppressed effects of parasympathetic stimulation and ACh release stronger than ATP. In conclusion, ATP and β-NAD control the heart at the postsynaptic and presynaptic levels via affecting the cardiac myocytes APs and ACh release. Postsynaptic and presynaptic effects of purines may be antagonistic and the latter demonstrates agedependence.

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Section: Age-specific Effects Of Atp and β-Nad In The Rat Heartsupporting

confidence: 50%

Extracellular ATP and β-NAD alter electrical properties and cholinergic effects in the rat heart in age-specific manner

Pustovit

Потехина

Ivanova

et al. 2019

Purinergic Signalling

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“…ATP was detected only in pear fruits, and its level was decreased by FD, just as demonstrated in their report. ATP is an unstable metabolite, which is rapidly hydrolyzed to ADP and phosphate 47, 48. Minute effects of FD on cellular structure may lead the hydrolysis of ATP.…”

Section: Discussionmentioning

confidence: 99%

Effects of freeze‐drying of samples on metabolite levels in metabolome analyses

Oikawa¹,

Otsuka²,

Yamaguchi

et al. 2011

J of Separation Science

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Freeze-drying (FD) is a useful technique for removing water from biological tissues, such as food samples. Cellular components freeze at once, and the ice sublimates under conditions of high vacuum and low temperatures. Because biological activity is restricted during FD, the degradation of cellular metabolites is often believed to be limited. However, the cellular structure is damaged by several factors, such as the increase in cell volume during freezing, and this has serious effects on the levels of some cellular metabolites. We studied these effects of FD on metabolite levels when using it as a sample preparation step in metabolome analysis. We observed significant decreases in the levels of some metabolites, such as succinate and choline, in Arabidopsis and pear, respectively. We also found that the effects of FD on certain metabolite levels differed between Arabidopsis plants and pear fruits. These results suggest that it is necessary to confirm the metabolite recovery in each sample species when FD is used for sample preparation.

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Effect ofRheedia longifoliaLeaf Extract and Fractions on the P2X₇ReceptorIn Vitro: Novel Antagonists?

Santos

Fidalgo-Neto

Faria

et al. 2011

Journal of Medicinal Food

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Recently, the P2X(7) receptor has been reported to be associated with chronic inflammatory and neuropathic pain. Because Rheedia longifolia extract has analgesic and anti-inflammatory activity, we evaluated the in vitro inhibitory potential of methanol extract and fractions from its leaves on the P2X(7) purinergic receptor. The activity of P2X(7) was studied with a dye uptake assay and with the whole-cell patch clamp technique in mouse peritoneal macrophages treated with methanol extract of R. longifolia leaves and fractions. The dye uptake was evaluated by flow cytometry and fluorescence microscopy. The R. longifolia extract and some fractions showed an inhibitory effect on the P2X(7) purinergic receptor in a dose-dependent manner. The ethyl acetate fraction exhibited the most potent inhibitory effects. The methanol extract and the butanol fraction showed the same inhibitory effects, despite their lower potency compared with the other fractions. The R. longifolia extract and some of its fractions may be anti-inflammatory because of their inhibitory effect on the P2X(7) receptor. Further investigation is needed to determine the pattern of inhibition and selectivity. Chromatographic analysis indicated the presence of bisflavonoids in the methanol extract fractions. A member of this chemical family is the most probable active compound responsible for the P2X(7) inhibitory effects present in the R. Longifolia extract and fractions.

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Effect of ATP and its analogs on contractility of rat myocardium during ontogeny

Cited by 4 publications

References 6 publications

Extracellular ATP and β-NAD alter electrical properties and cholinergic effects in the rat heart in age-specific manner

Extracellular ATP and β-NAD alter electrical properties and cholinergic effects in the rat heart in age-specific manner

Effects of freeze‐drying of samples on metabolite levels in metabolome analyses

Effect ofRheedia longifoliaLeaf Extract and Fractions on the P2X₇ReceptorIn Vitro: Novel Antagonists?

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Effect of ATP and its analogs on contractility of rat myocardium during ontogeny

Cited by 4 publications

References 6 publications

Extracellular ATP and β-NAD alter electrical properties and cholinergic effects in the rat heart in age-specific manner

Extracellular ATP and β-NAD alter electrical properties and cholinergic effects in the rat heart in age-specific manner

Effects of freeze‐drying of samples on metabolite levels in metabolome analyses

Effect ofRheedia longifoliaLeaf Extract and Fractions on the P2X7ReceptorIn Vitro: Novel Antagonists?

Contact Info

Product

Resources

About

Effect ofRheedia longifoliaLeaf Extract and Fractions on the P2X₇ReceptorIn Vitro: Novel Antagonists?