Effect of Ascorbic Acid on Lipid Autoxidation in a Model Food System

Ueda, Shinji; Hayashi, Tateki; Namiki, Mitsuo

doi:10.1080/00021369.1986.10867337

Cited by 26 publications

(25 citation statements)

References 2 publications

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“…Hence, the data obtained in previous studies may have provoked a slightly inaccurate interpretation of the results [6,22]. In photooxidation the amount of nonconjugated hydroperoxides formed is usually 30-40% and that of conjugated hydroperoxides is 60-70% [17,18]. According to Ohshima et al [17] the distribution of hydroperoxide isomers depend on the amount of total hydroperoxides.…”

Section: Discussionmentioning

confidence: 97%

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Carotenoids as antioxidants to prevent photooxidation

Viljanen

Sundberg

Ohshima

et al. 2002

Eur. J. Lipid Sci. Technol.

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Carotenoids as antioxidants to prevent photooxidationCarotenoids act as antioxidants in photooxidation by quenching singlet oxygen or triplet sensitizer. The antioxidant activities of β-carotene, lutein and lycopene during photooxidation were investigated by following the formation of methyl linoleate isomeric hydroperoxides. The hydroperoxide formation and the isomeric distribution were determined using high-performance liquid chromatography and post-column detection with diphenyl-1-pyrenyl phosphine (DPPP). DPPP reacts with both the conjugated and nonconjugated hydroperoxides formed in photooxidation to give fluorescing DPPPoxides. Photooxidation with or without added β-carotene, lutein and lycopene (10, 20 and 40 ppm) were carried out at +3°C under 2000 lx. All the studied carotenoids were potential antioxidants during photooxidation and their antioxidant activities were concentration-dependent. There were no significant differences in the antioxidant activities of lycopene and β-carotene at a concentration of 10 ppm. At a concentration of 20 ppm β-carotene was a better antioxidant than lycopene or lutein, and at a concentration of 40 ppm lycopene exerted a better antioxidant activity than β-carotene or lutein. There were no significant differences between lycopene and lutein at a concentration of 20 ppm. The results also showed that carotenoids had no effect on the distribution of isomeric hydroperoxides indicating that the antioxidant mechanism of carotenoids during photooxidation does not involve hydrogen donation. All carotenoids were consumed during photooxidation. At a higher concentration (40 ppm) lycopene was more stable than the other tested carotenoids. That contributed most likely to it having a better antioxidant activity than β-carotene and lutein.

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Section: Discussionmentioning

confidence: 97%

“…There to the method described by Ueda et al was slightly modified [18]. The PV given is the average of three measured values.…”

Section: Peroxide Value Measurementmentioning

confidence: 99%

Carotenoids as antioxidants to prevent photooxidation

Viljanen

Sundberg

Ohshima

et al. 2002

Eur. J. Lipid Sci. Technol.

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“…PV were measured by the official ferric thiocyanate method of the International Dairy Federation (IDF) (13) as modified by Ueda et al (14). Iso-hexane (HPLC grade, Fisons, Loughborough, England) was used instead of n-hexane to dissolve the rapeseed oil (Undeland, I., H. Lingnert, and M. Stading, unpublished data).…”

Section: Methodsmentioning

confidence: 99%

Influence of oxygen and copper concentration on lipid oxidation in rapeseed oil

Andersson

Lingnert

1998

J Americ Oil Chem Soc

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The influence of oxygen concentration and copper on lipid oxidation in rapeseed oil during storage at 40°C was investigated. The oil was stored in air, or with 1.1%, 0.17%, or 0.04% oxygen in the headspace, and 70 or 0.07 ppm copper was added. Volatile oxidation products and oxygen consumption were monitored. Addition of 70 ppm copper to the sample in air resulted in a 70-fold higher hexanal concentration after 35 d of storage, compared to the sample without added copper. The addition of 0.07 ppm copper to the sample stored in air gave a doubled hexanal concentration, compared to the sample without copper, after 35 d of storage. For the samples with 70 ppm copper at 0.17% and 0.04% oxygen, all oxygen was consumed after 7 d of storage. The results show the importance of minimizing the oxygen available for oxidation, especially when pro-oxidants are present. In the sample with 70 ppm added copper, in air, the hexanal increase was 65 times larger than for the same sample in 0.04% oxygen. A comparison of the effect of oxygen or copper on oxidation shows that the addition of 70 ppm copper to the 0.04% oxygen sample gave the same increase in hexanal content as an oxygen increase to 0.17%. JAOCS 75, 1041-1046 (1998). FIG. 6. Hexanal concentration (ng/L HS) of the samples without copper in air and with 0.07 ppm copper in air, in 1.1% O 2 , in 0.17% O 2 , and in 0.04% O 2 . Error bars indicate standard deviations from three measurements. See Figure 5 for abbreviation. FIG. 7. 2-Hexenal concentration (ng/L HS) of the samples without and with 70 ppm copper at four oxygen levels as in Figure 5. Error bars indicate standard deviations from three measurements. See Figure 5 for abbreviation.

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“…The 10% oil-in-water emulsion samples were oxidized uncovered for 4 d in 300-mL flasks at 25°C in the dark in a shaker water bath (Gyrotory Water Bath Shaker Model G76, New Brunswick Scientific Co., Edison, NJ). The effect of β-carotene and α-and γ-tocopherol on autoxidation of the emulsified RSO TAG was followed by measuring PV with the ferric thiocyanate method (21,22) and the decomposition of lipid hydroperoxides into volatile aldehydes. Hexanal, 2,4-heptadienal, and heptenal were determined as their DNPH derivatives by HPLC.…”

Section: Methodsmentioning

confidence: 99%

“…Commercial RSO was a gift from Van den Bergh Foods (Helsinki, Finland).The TAG fraction was purified from RSO by the chromatographic method described by Lampi et al (20). The RSO TAG fraction was characterized by its tocopherol composition as analyzed by HPLC as described by Haila and Heinonen (18) and by its peroxide value (PV) as measured by the ferric thiocyanate method (21,22). The determination limit of tocopherols was 1 µg/g.…”

Section: Methodsmentioning

confidence: 99%

Inhibition of oxidation in 10% oil‐in‐water emulsions by β‐carotene with α‐ and γ‐tocopherols

Heinonen

Haila

Lampi

et al. 1997

J Americ Oil Chem Soc

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The effects of low concentrations of β-carotene, α-, and γ-tocopherol were evaluated on autoxidation of 10% oil-in-water emulsions of rapeseed oil triacylglycerols. At concentrations of 0.45, 2, and 20 µg/g, β-carotene was a prooxidant, based on the formation of lipid hydroperoxides, hexanal, or 2-heptenal. In this emulsion,1.5, 3, and 30 µg/g of γ-tocopherol, as well as 1.5 µg/g of α-tocopherol, acted as antioxidants and inhibited both the formation and decomposition of lipid hydroperoxides. Moreover, at a level of 1.5 µg/g, γ-tocopherol was more effective as an antioxidant than α-tocopherol. At levels of 0.5 µg/g, both α-and γ-tocopherol significantly inhibited the formation of hexanal but not the formation of lipid hydroperoxides. Oxidation was effectively retarded by combinations of 2 µg/g β-carotene and 1.5 µg/g γ-or α-tocopherol. The combination of β-carotene and α-tocopherol was significantly better in retarding oxidation than α-tocopherol alone. While γ-tocopherol was an effective antioxidant, a synergistic effect between β-carotene and γ-tocopherol could not be shown. The results indicate that there is a need to protect β-carotene from oxidative destruction by employing antioxidants, such as α-and γ-tocopherol, should β-carotene be used in fat emulsions.

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Effect of Ascorbic Acid on Lipid Autoxidation in a Model Food System

Cited by 26 publications

References 2 publications

Carotenoids as antioxidants to prevent photooxidation

Carotenoids as antioxidants to prevent photooxidation

Influence of oxygen and copper concentration on lipid oxidation in rapeseed oil

Inhibition of oxidation in 10% oil‐in‐water emulsions by β‐carotene with α‐ and γ‐tocopherols

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