Effect of antimicrobial agents incorporated into resilient denture relines on the <i>Candida albicans</i> biofilm

Bueno, M. G.; Urban, Vanessa Migliorini; Barbério, G.; Silva, Wander José da; Porto, Vinícius Carvalho; Pinto, Luiz Fernando Spinelli; Neppelenbroek, Karin Hermana

doi:10.1111/odi.12207

Cited by 61 publications

(94 citation statements)

References 45 publications

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“…The significant decrease of the SMIC50 suggested that the sensitivity of the biofilm to the antifungal drugs is dramatically increased after plasma treatment. Drug resistance is another main issue for the failure treatment of DS [4,41]. The drug susceptibility of Candida albicans biofilms prepared on PMMA denture base resin to both AMB and FLU increased 90 % after only 8 min plasma treatment, while increment of 10 % of the susceptibility to antifungal drugs has been recognized as a breakthrough [42], which prompts the superiority of cold plasma treatment for the Candida albicans biofilms formed on denture base resin.…”

Section: Discussionmentioning

confidence: 99%

“…Pharmacotherapy including chlorhexidine, amphotericin B and fluconazole has also been introduced for DS treatment [3]. However, antifungal drug resistance is observed for the treatment of denture stomatitis in the denture wearers [4]. Thus, the urgent requirement for effective treatment of the Candida albicans biofilms is more than obvious.…”

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

Inactivation of Candida albicans Biofilms on Polymethyl Methacrylate and Enhancement of the Drug Susceptibility by Cold Ar/O2 Plasma Jet

Wang

Sun

Pan

et al. 2015

Plasma Chem Plasma Process

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The antimicrobial effects of the cold plasma for the dental pathogenic microorganism propose a promising approach to the Denture Stomatitis (DS) treatment. However, it is crucial to understand that the complexity of the biofilm microenvironment may compromise the efficiency of the therapy. As one of the major issue for DS, Candida albicans biofilms (ATCC10231) formed on denture base resins were treated by cold Ar/O 2 (2 %) plasma jet. Spatial viability of the biofilms was investigated with confocal scanning laser microscopy through evaluating their inside cross-section properties. Results showed Candida albicans biofilms with thickness of *100 lm was completely inactivated by 8 min plasma treatment. Morphology change of the fungi was also observed by the scanning electron microscopy. Drug susceptibilities, the sessile minimum inhibitory concentration (SMIC50) of the biofilm for amphotericin B and fluconazole were decreased from [32 and [256 lg/mL to 8 and 64 lg/mL after 1 min's plasma treatment, respectively. The reactive species produced from plasma were monitored by optical emission spectroscopy. The successfully inactivation of Candida albicans biofilms and the significant enhancement of its drug susceptibilities induced by the plasma released reactive species propose a promising strategy for the treatment of DS caused by drug-resistant Candida albicans biofilms.

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Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Inactivation of Candida albicans Biofilms on Polymethyl Methacrylate and Enhancement of the Drug Susceptibility by Cold Ar/O2 Plasma Jet

Wang

Sun

Pan

et al. 2015

Plasma Chem Plasma Process

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“…Alternative formulations have been proposed to enhance the antimicrobial action of natural products, including chewing gum, 28 sustained drug release systems 29,30 and denture relining with resilient materials. 31 Figure 3. CFU / specimen of S. mutans ATCC 35688 biofilm in dental enamel without treatment (Negative Control, n = 11), treated with citral (n = 12), or mouthwash containing chlorhexidine digluconate at 1.2 mg ml -1 (CHX, positive control, n = 12).…”

Section: Discussionmentioning

confidence: 99%

Cymbopogon citratus essential oil: effect on polymicrobial caries-related biofilm with low cytotoxicity

Oliveira¹,

Borges²,

Brighenti

et al. 2017

Braz. oral res.

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The objective of this study was to evaluate the effects of Cymbopogon citratus essential oil and its main compound (citral) against primary dental colonizers and caries-related species. Chemical characterization of the essential oil was performed by gas chromatography/mass spectroscopy (GC/MS), and the main compound was determined. Antimicrobial activity was tested against Actinomyces naeslundii, Lactobacillus acidophilus, S. gordonii, S. mitis, S. mutans, S. sanguinis and S. sobrinus. Minimum inhibitory and bactericide concentrations were determined by broth microdilution assay for streptococci and lactobacilli reference, and for clinical strains. The effect of the essential oil on bacterial adhesion and biofilm formation/disruption was investigated. Negative (without treatment) and positive controls (chlorhexidine) were used. The effect of citral on preformed biofilm was also tested using the same methodology. Monospecies and microcosm biofilms were tested. ANOVA or Kruskal-Wallis tests were used (α=0.05). Cytotoxicity of the essential oil to human keratinocytes was performed by MTT assay. GC/MS demonstrated one major component (citral). The essential oil showed an inhibitory effect on all tested bacterial species, including S. mutans and L. acidophilus. Essential oil of C. citratus (10X MIC) reduced the number of viable cells of lactobacilli and streptococci biofilms (p < 0.05). The essential oil inhibited adhesion of caries-related polymicrobial biofilm to dental enamel (p < 0.01). Citral significantly reduced the number of viable cells of streptococci biofilm (p < 0.001). The essential oil showed low cytotoxicity to human keratinocytes. Based on these findings, this study can contribute to the development of new formulations for products like mouthwash, against dental biofilms.

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“…However, it has been shown that the incorporation of drugs at commercially available concentrations in soft liners may affect its morphological structure (11) and properties such as tensile strength (9,12), water sorption (13), modulus of elasticity, and weight (9), hardness (9,14) and roughness (14). In the search for antifungal concentrations compatible with the properties of these materials, Bueno et al (15) determined the minimum inhibitory concentrations (MICs) for C. albicans biofilm of antifungal agents (nystatin, miconazole, ketoconazole, itraconazole and chlorhexidine) incorporated in temporary resilient liners (Trusoft and Softone). The authors observed that all drugs added to both materials were able to inhibit 90% or more of C. albicans biofilm in up to 14 days of incubation.…”

Section: Introductionmentioning

confidence: 99%

Surface Properties of Temporary Soft Liners Modified by Minimum Inhibitory Concentrations of Antifungals

et al. 2017

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albicans biofilm on the hardness and roughness of temporary denture soft liners. Specimens (n=8; 36×7×6 mm) of tissue conditioner (Softone) and resilient liner (Trusoft) were produced either without (control) or with incorporation of drugs at MICs: nystatin (0.032 g/mL), chlorhexidine diacetate (0.064 g/mL), ketoconazole (0.128 g/mL), miconazole (0.256 g/mL) and itraconazole (0.256 g/mL). Specimens were stored in distilled water at 37 °C for 24 h, 7 days and 14 days prior to the hardness/roughness measurements. Data were analyzed by 3-way ANOVA and Tukey HSD test (α=0.05). The addition of the antifungals into both materials demonstrated no evident hardness change or decrease of this property compared with the control, except for miconazole in Softone, which increased the hardness after 14 days (p=0.003). The addition of nystatin into both materials, chlorhexidine in Trusoft and ketoconazole in Softone resulted in no significant changes of roughness compared with the control, after 7 days and 14 days (p>0.05). In these periods, itraconazole increased the roughness of both materials (p<0.001). The addition of all antifungals, except for the miconazole in Softone, resulted in no deleterious effects on the materials' hardness over the evaluation time. The MICs of nystatin in both temporary soft lining materials, ketoconazole in Softone and chlorhexidine in Trusoft resulted in no deleterious effects for roughness up to 14 days.

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Effect of antimicrobial agents incorporated into resilient denture relines on the Candida albicans biofilm

Abstract: The antimicrobials incorporated in the resilient materials inhibited fungal growth during 14 days, with lower MICs for nystatin and chlorhexidine.

Cited by 61 publications

References 45 publications

Inactivation of Candida albicans Biofilms on Polymethyl Methacrylate and Enhancement of the Drug Susceptibility by Cold Ar/O2 Plasma Jet

Inactivation of Candida albicans Biofilms on Polymethyl Methacrylate and Enhancement of the Drug Susceptibility by Cold Ar/O2 Plasma Jet

Cymbopogon citratus essential oil: effect on polymicrobial caries-related biofilm with low cytotoxicity

Surface Properties of Temporary Soft Liners Modified by Minimum Inhibitory Concentrations of Antifungals

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