Surface Properties of Temporary Soft Liners Modified by Minimum Inhibitory Concentrations of Antifungals

Abstract

albicans biofilm on the hardness and roughness of temporary denture soft liners. Specimens (n=8; 36×7×6 mm) of tissue conditioner (Softone) and resilient liner (Trusoft) were produced either without (control) or with incorporation of drugs at MICs: nystatin (0.032 g/mL), chlorhexidine diacetate (0.064 g/mL), ketoconazole (0.128 g/mL), miconazole (0.256 g/mL) and itraconazole (0.256 g/mL). Specimens were stored in distilled water at 37 °C for 24 h, 7 days and 14 days prior to the hardness/roughness measurements. Data were analyzed by 3-way ANOVA and Tukey HSD test (α=0.05). The addition of the antifungals into both materials demonstrated no evident hardness change or decrease of this property compared with the control, except for miconazole in Softone, which increased the hardness after 14 days (p=0.003). The addition of nystatin into both materials, chlorhexidine in Trusoft and ketoconazole in Softone resulted in no significant changes of roughness compared with the control, after 7 days and 14 days (p>0.05). In these periods, itraconazole increased the roughness of both materials (p<0.001). The addition of all antifungals, except for the miconazole in Softone, resulted in no deleterious effects on the materials' hardness over the evaluation time. The MICs of nystatin in both temporary soft lining materials, ketoconazole in Softone and chlorhexidine in Trusoft resulted in no deleterious effects for roughness up to 14 days.

Mechanical and Surface Properties of Resilient Denture Liners Modified with Chitosan Salts

“…Urban et al reported that the addition of antimicrobial agents to an acrylic-based resilient denture liner did result in higher SHA values compared to the control group after 14 days [20]. On the contrary, Bueno et al reported significant decreases in SHA after 14 days for all modifications of an acrylic-based resilient denture liner with antifungals compared to the control group [43]. The tendency of the latter result was confirmed by the present study.…”

“…Changes in SHA when adding antimicrobial particles were also reported by other authors [20,21,43]. Chladek et al modified a silicone resilient denture liner and found SHA values to decrease with increasing concentration of silver nanoparticles [21] Contrary, the present study showed SHA values of the modified silicone resilient denture liner increasing or showing similar values compared to control group.…”

“…In the case of the acrylic-based resilient denture liner, control group and all modifications with CS-HCl as well as with 0.1% and 0.2% CS-G became less rough over time or ended up with similar values at 24 h and 30 days. Similar effects for acrylic-based resilient denture liners were shown by Bueno et al and Urban et al [20,43].…”

“…These studies show promising antifungal activity of modified materials but do not evaluate mechanical properties. To the best of the authors’ knowledge, only studies describing modifications of resilient denture liners with other particles (silver nanoparticles; nystatin, miconazole, ketoconazole, itraconazole, chlorhexidine) evaluated mechanical properties [20,21,43]. Furthermore, no studies were found describing the addition or modification of resilient denture liners with CS salts such as CS-G and CS-HCl.…”

Avaliação da adição de antimicrobianos em reembasador resiliente temporário no tratamento da estomatite protética: um estudo clínico randomizado

“…A incorporação de CIMs de nistatina e diacetato de clorexidina em um reembasador resiliente temporário para base de prótese (Trusoft) mostrou efetividade in vitro na inibição do biofilme de C. albicans (BUENO et al, 2015), sem alterar deleteriamente as propriedades físicas, mecânicas e biológicas do material ao longo de sua vida útil (14 dias) (SÁNCHEZ-ALIAGA et al, 2013;LIMA et al, 2016a;LIMA et al, 2016b;SÁNCHEZ-ALIAGA et al, 2016;SÁNCHEZ-ALIAGA et al, 2016;BUENO et al, 2017;NEPPELENBROEK et al, 2018). Ainda, no decorrer desse período, tal sistema de liberação sustentada de fármacos apresentou biocompatibilidade com a mucosa palatina de ratos (HOTTA et al, 2016;HOTTA et al, 2019 -gel (CATALÁN et al, 2008;HONG et al, 2012).…”