Effect of Antibacterial Dental Adhesive on Multispecies Biofilms Formation

Zhang, K.; Wang, S.; Zhou, Xuedong; Xu, Hockin H.K.; Weir, Michael D.; Ge, Yichen; Li, M.; Li, Y.; Xu, Xin; Zheng, Lan; Cheng, Lei

doi:10.1177/0022034515571416

Cited by 107 publications

(144 citation statements)

References 37 publications

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“…All three-dimensional reconstructions of biofilms were performed with Imaris 7.0.0 (Bitplane, Zürich, Switzerland). The vertical distribution analyses were performed with LAS AF Lite (Leica), and the calculation of bacteria/EPS biomass was performed with COMSTAT (http://www.imageanalysis.dk) [31]. …”

Section: Methodsmentioning

confidence: 99%

Antimicrobial peptide GH12 suppresses cariogenic virulence factors of Streptococcus mutans

Wang

Jiang

et al. 2018

Journal of Oral Microbiology

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Cariogenic virulence factors of Streptococcus mutans include acidogenicity, aciduricity, and extracellular polysaccharides (EPS) synthesis. The de novo designed antimicrobial peptide GH12 has shown bactericidal effects on S. mutans, but its interaction with virulence and regulatory systems of S. mutans remains to be elucidated. The objectives were to investigate the effects of GH12 on virulence factors of S. mutans, and further explore the function mechanisms at enzymatic and transcriptional levels. To avoid decrease in bacterial viability, we limited GH12 to subinhibitory levels. We evaluated effects of GH12 on acidogenicity of S. mutans by pH drop, lactic acid measurement and lactate dehydrogenase (LDH) assay, on aciduricity through survival rate at pH 5.0 and F1F0-ATPase assay, and on EPS synthesis using quantitative measurement, morphology observation, vertical distribution analyses and biomass calculation. Afterwards, we conducted quantitative real-time PCR to acquire the expression profile of related genes. GH12 at 1/2 MIC (4 mg/L) inhibited acid production, survival rate, EPS synthesis, and biofilm formation. The enzymatic activity of LDH and F1F0-ATPase was inhibited, and ldh, gtfBCD, vicR, liaR, and comDE genes were significantly downregulated. In conclusion, GH12 inhibited virulence factors of S. mutans, through reducing the activity of related enzymes, downregulating virulence genes, and inactivating specific regulatory systems.

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Section: Methodsmentioning

confidence: 99%

Antimicrobial peptide GH12 suppresses cariogenic virulence factors of Streptococcus mutans

Wang

Jiang

et al. 2018

Journal of Oral Microbiology

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“…Generally speaking, however, with the increase of the amount of antibacterial agent, the mechanical properties and biocompatibility of the material will decrease. QACs belong to a kind of nonrelease antibacterial agent, and the antibacterial effect can be achieved when the dosage is relatively large 32,[44][45][46][47] . While the release antimicrobial agents can achieve better antibacterial effect at a low concentration, however, their extending antibacterial efficiency is low and they may be more toxic toward surrounding environments, the so-called "sudden release effect" [104][105][106][107] .…”

Section: Resultsmentioning

confidence: 99%

“…And then, dimethylaminododecyl methacrylate (DMADDM), NAg, and NACP were incorporated into above adhesive system together and they found that modified bonding agent yielded potent and long lasting antimicrobial properties, and much stronger bond strength after 6 months of water aging than a commercial control 43) . Meanwhile, 5 wt% DMADDM was incorporated into Clearfil SE Bond by Zhang et al It had been observed that adhesives containing DMADDM inhibited the growth, lactic acid production, and Exopolysaccharides (EPS) metabolism of S. mutans biofilm at different stages, with no adverse effect on its dentin adhesive bond strength 44,45) . Besides that, Pupo et al 46,47) performed a study incorporating of quaternary ammonium methacrylate polymer (QAMP) at 5% by mass to Clearfil SE Bond as experimental group, Clearfil SE Bond as negative control group, and Clearfil Protect Bond as positive control group.…”

Section: Adhesive Systemmentioning

confidence: 99%

“…The antibacterial effect against two E. faecalis strains (ATCC and an endodontic isolate) were assessed, and results had shown that addition of QPEI nanoparticles improved the killing ability of PCS against biofilms of both E. faecalis strains and the effects of AH Plus on the biomass of biofilms from the ATCC strain. Moreover, there were no relevant changes in physicochemical and 95) quaternary ammonium polyethyleneimine QPEI unpolymerizable composite resin 24) , endodontic materials [89][90][91] quaternary ammonium dimethacrylate QADM/IMQ polymerizable composite resin [25][26][27]32) , adhesive system 42) 2-methacryloxylethyl dodecyl methyl ammonium bromide MAE-DB polymerizable composite resin 28,29) 2-methacryloxylethyl hexadecyl methyl ammonium bromide MAE-HB polymerizable composite resin 28,29) 2-Dimethyl-2-dodecyl-1-methacryloxyethyl ammonium iodine DDMAI polymerizable composite resin 30) quaternary ammonium methacrylate QAM polymerizable composite resin 31) , adhesive system 5,53,54) 2-methacryloyloxyethyl phosphorylcholine MPC polymerizable composite resin 33) dimethylaminododecyl methacrylate DMADDM polymerizable adhesive system [43][44][45] quaternary ammonium methacrylate polymer QAMP unpolymerizable adhesive system [46][47][48] methacryloxylethyl cetyl ammonium chloride DMAE-CB polymerizable adhesive system [49][50][51][52] benzalkonium chloride BAC unpolymerizable adhesive system [55][56][57] , glass ionomer cement 73,74) , endodontic materials 87) poly-2-methacry...…”

Section: Endodontic Materialsmentioning

confidence: 99%

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Quaternary ammonium compounds in dental restorative materials

Zhang

Chen

et al. 2018

Dental Materials Journal

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Quaternary ammonium compounds (QACs) have been used widely in medicine, owing to their antimicrobial properties. They also have strong permeability, stable performance, low skin irritation, low toxicity, low corrosion, lasting biological effects and so on, comparing with other antimicrobial agents. At the end of last century, polymerizable quaternary ammonium antimicrobial monomers had been synthesized and applied in dentistry, in order to reduce or prevent microbial biofilm formation on dental materials surfaces. This review aims to discuss the current progress of QACs applied in composite resin, adhesive systems, acrylic resin, glass ionomer cement and endodontic materials, regarding to their antimicrobial potential, mechanical properties, and biocompatibility.

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“…QAMs exert a contact-killing mechanism and have been shown to effectively kill bacteria and inhibit biofilm growth, not only on single-species biofilms, but also on multispecies biofilms [Zhang et al, 2015;Wang et al, 2016]. In addition, the antimicrobial properties are considered to be related to chain lengths and charge densities [Zhou et al, , 2014.…”

Section: Evaluation Of Novel Anticaries Adhesive In a Secondary Cariementioning

confidence: 99%

Evaluation of Novel Anticaries Adhesive in a Secondary Caries Animal Model

et al. 2017

Caries Res

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(1) secondary caries was successfully produced in rats; (2) there was a correlation between the modified Keyes scoring method and micro-CT in the evaluation of the secondary caries; (3) the adhesive containing DMADDM significantly reduced both LD and ML (according to micro-CT), and also lowered the scores (based on the modified Keyes scoring method). This suggests that the novel DMADDM adhesive could perform an anticaries function in vivo via the secondary caries animal model which was also developed and testified in research. Secondary caries is one of the major reasons leading to the failure of caries restoration treatment. As a solution, anticaries adhesives perform well in biofilm inhibition in vitro. However, the lack of secondary caries animal models limits the evaluation of anticaries adhesives in vivo. Secondary caries is one of the main reasons for restoration failure with a heavy economic burden [Sakaguchi, 2005;Kasraei et al., 2017]. While many factors facilitate the development of secondary caries, oral bacteria and acid production are the initiators of dental caries [Mjor Keywords Antibacterial material · Bonding system · Dimethylaminododecyl methacrylate · Keyes animal model · Micro-CT · Tooth restoration AbstractWe investigated the anticaries properties of an adhesive containing dimethylaminododecyl methacrylate (DMADDM) in vivo via a secondary caries animal model. Cavities were prepared in the maxillary first molars of Wistar rats. DMADDM-containing adhesives were applied on one side and commercial adhesives on the opposite side as a control. After a 3-week feeding period to induce secondary caries, the molars were harvested for the evaluation of the secondary caries. Lesion depth (LD) and mineral loss (ML) were measured via a micro-CT method, and a modified Keyes scoring method yielded scores for the caries lesions. Statistical analysis was divided into 2 parts: a correlation analysis between 2 evaluations with one-way ANOVA and a least-significant differences (LSD) test, and an evaluation of anticaries adhesives with a paired samples t test. The results showed that:

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Effect of Antibacterial Dental Adhesive on Multispecies Biofilms Formation

Cited by 107 publications

References 37 publications

Antimicrobial peptide GH12 suppresses cariogenic virulence factors of Streptococcus mutans

Antimicrobial peptide GH12 suppresses cariogenic virulence factors of Streptococcus mutans

Quaternary ammonium compounds in dental restorative materials

Evaluation of Novel Anticaries Adhesive in a Secondary Caries Animal Model

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