Effect of anti-inflammatory agents on the integration of autogenous bone graft and bovine bone devitalized matrix in rats

Silva, Roberto Antoniolli da; Fagundes, Djalma José; Silva, Andréia Conceição Milan Brochado Antoniolli; Sisti, Karin Ellen; Carvalho, Themis Maria Milan Brochado de; Silva, Daniel Nunes e

doi:10.1590/s0102-86502008000200006

Cited by 11 publications

(8 citation statements)

References 15 publications

(35 reference statements)

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“…However, some important clinical limitations are associated with AB, such as the need for a donor site, donor site morbidity, increased operative time, blood loss, additional cost, and limited availability 1,15,17 . In this context, alloplastic biomaterials have been increasingly investigated and considered as effective alternative bone substitutes.…”

Section: Discussionmentioning

confidence: 99%

Alpha-tricalcium phosphate cement in the reconstruction of bone defects in rats

et al. 2011

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Purpose:To evaluate the ability of a mixture of α-TCP and autogenous bone (AB) vs. α-TCP alone and AB alone to promote new bone formation and tissue repair in bone defects. Methods: Bone defects surgically created in 15 male Wistar rats were divided into four groups: Group I (AB), Group II (α-TCP), Group III (α-TCP+AB assessed by light microscopy), and Group IV (α-TCP+AB assessed by scanning electron microscopy). Bone repair findings were assessed at 30, 60, and 120 days postoperatively. Results: The histological findings obtained in Groups I (p=0.459), II (p=0.368), and III (p=0.459) and at 30 days (p=0.717), 60 days (p=0.717), and 120 days (p=0.779) did not show statistically significant differences. Scanning electron microscopy revealed direct contact between the α-TCP+AB implant and the bone tissue at 120 days. Conclusion: The α-TCP implant is effective alternative bone substitutes for the treatment of critical size bone defects. Key words: Bone Substitutes. Bone Transplantation. Biocompatible Materials. Rats. RESUMOObjetivo: Avaliar a capacidade da mistura de α-TCP e osso autógeno (OA) Vs somente α-TCP e somente OA em promover a neoformação óssea e reparo tecidual em defeitos ósseos. Métodos: Foram criados cirurgicamente defeitos ósseos em 15 ratos Wistar machos, distribuídos em quatro grupos: Grupo I (OA), Grupo II (α-TCP), Grupo III (α-TCP + OA avaliado por microscopia de luz) e Grupo IV (α-TCP + OA avaliado por microscopia eletrônica de varredura). Os resultados da reparação óssea foram avaliados em 30, 60 e 120 dias. Resultados: os achados histológicos obtidos nos grupos I (p = 0,459), II (p = 0,368) e III (p = 0,459) e aos 30 dias (p = 0,717), 60 dias (p = 0,717), e 120 dias (p = 0,779) não apresentaram diferenças estatisticamente significativas. A análise por microscopia eletrônica de varredura revelou contacto direto entre o implante de α-TCP + AO e o tecido ósseo aos 120 dias. Conclusão: O implante de α-TCP é alternativa eficaz como substitutos ósseos para o tratamento de defeitos ósseos de tamanho crítico. Descritores: Substitutos Ósseos. Enxerto Ósseo. Materiais Biocompatíveis. Ratos. -Acta Cirúrgica Brasileira -Vol. 26 (2) 2011Júnior ANS et al.

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Section: Discussionmentioning

confidence: 99%

Alpha-tricalcium phosphate cement in the reconstruction of bone defects in rats

et al. 2011

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“…Entre os substitutos ósseos xenógenos, os mais utilizados atualmente são os de origem bovina, pois em humanos estes materiais possuem uma grande propriedade de osteocondução (Oliveira et al, 1999), apresentam uma resistência biomecânica muito similar à do osso humano e com tratamentos adequados durante sua obtenção evitam respostas imunológicas ou inflamatórias adversas (Sicca et al, 2000;Wataha, 2001;Wenz, Oesch e Horst, 2001;Meyer, Floerkemeier e Windhgen, 2008). Estudos in vivo têm demonstrado a viabilidade do osso xenogênico em defeitos ósseos, sejam eles desproteinizado ou matriz óssea orgânica (Pinheiro et al, 2008;Betti et al, 2011;Bernabe et al, 2012), desmineralizados ou matriz óssea inorgânica (Banjar e Mealey, 2013;Bigham-Sadegh et al, 2013;Liang et al, 2014) e desvitalizados ou mistos (Silva et al, 2008).…”

Section: 35unclassified

Avaliação histomorfométrica da biocompatibilidade do enxerto bovino misto (OrthoGen®) em tecido subcutâneo e o potencial osteogênico em defeito ósseo craniano

Oliveira¹

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Histomorphometric evaluation of biocompatibility of integral bovine graft (Orthogen ®) implanted in subcutaneous tissue and osteogenic potential in cranial bone defect The current development of bone graft materials with the potential to promote the complete closure of a critical size bone defect has raised questions as to its biological activity. An option of bone material used, are animal bone grafts since the human bone have similar physicochemical properties. Among the materials is the integral bone substitute of bovine origin, which preserves the organic and inorganic compound of the bone tissue, has been proposed and used as bone graft. The aim of this study was to evaluate the biocompatibility and osteogenic potential of a new integral bone substitute Orthogen TM (Baumer S.A.) in the form of particles (OGp) and block (OGb). For biocompatibility evaluation, 100mg Orthogen TM was implanted into dorsal subcutaneous pocket of rat (n= 25) and the reactional tissue was analyzed at 7, 14, 21, 30 and 60 days (n=5animals/period) after implantation. For osteogenic potential evaluation, 100mg Orthogen TM was implanted into critical-size defect in parietal bones of rat (n=30) and the bone formation, biomaterial reabsorption, connective tissue formation and osteoclast activity was evaluated at 1, 3 and 6 months (n=10/period) after implantation. Radiographic and histomorphometrical analysis showed that, in the subcutaneous tissue the OGb was more accepted by the host compared to OGp, with lower density of the multinucleated giant cells (OGb 0.23% vs. OGp 2.19%) and consequently a lower rate of matrix resorption (OGb 13%, vs. OGp 38%). In both implants was not found focus of inflammatory infiltrated composed by polymorphonuclear leucocytes, lymphocytes and plasmocytes. In rat calvaria the OGb showed lower rate of reabsorption and more volume of bone formation compared to OGp after 6 months (OGb 70 mm 3 vs OGp 17 mm 3). Based on experimental models used in this study we concluded that both forms of the Orthogen TM was biocompatible in subcutaneous tissue, however, its form of porous block promoted greater bone formation and has a higher osteogenic capacity than the particle shape, in the evaluated experimental model.

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“…Στις περιπτώσεις που προκλήθηκε οστικό έλλειµµα, αυτό ήταν µικρής διαµέτρου ≤ 2mm και η περιοχή πρόκλησής του ήταν ο µηρός (Sassioto et al 2006, Krischak al. 2007, Silva et al 2008. Ερευνητές µελέτησαν την επίδραση της δικλοφενάκης στην οστική επούλωση σε γνάθους επίµυων µετά από εξαγωγή δοντιού ή µετά από ορθοδοντική µετακίνηση δοντιού.…”

Section: προσταγλαν∆ινεςunclassified

“…Παρόλα αυτά, υπάρχουν αρκετές διαφορές µεταξύ των διαφόρων µελετών. Οι περισσότεροι ερευνητές µελετούν τη βραχυχρόνια χορήγηση της δικλοφενάκης, η οποία κυµαίνεται από µία εφάπαξ δόση (Sassioto et al 2006) µέχρι 7-10 ηµέρες (Akman et al 2002, Yugoshi et al 2002, Krischak et al 2007, Silva et al 2008, Spiro et al 2010, κάποιοι άλλοι ερευνητές χορήγησαν την ουσία µακροχρόνια ( µέχρι 4 εβδοµάδες ) ή µελέτησαν την επίδρασή της στο οστό σε διάφορους χρόνους µελέτης (Beck et al 2003, Tiseo et al 2006, Krischak et al 2007, Retamoso et al 2010, Georgopoulou-karanikola et al, 2020).…”

Section: προσταγλαν∆ινεςunclassified

“…Οι Sassioto και συν. σε εργασία τους, µε σκοπό τη µελέτη της επίδρασης της χορήγησης ΜΣΑΦ στην επούλωση προκληθέντος οστικού ελλείµµατος 2mm στο µηρό επίµυων, χορήγησαν 1 Οι Silva και οι συνεργάτες, σε παρόµοιο µε το παραπάνω µοντέλο, και µε τη χρήση µοσχευµατικού υλικού, οδηγηθήκανε στο συµπέρασµα ότι η δικλοφενάκη επιβραδύνει την οστική επούλωση και σε µικρότερη αλλά συνεχόµενη ηµερήσια δόση 2mg/kg Β.Σ (Silva et al 2008)…”

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Η Επίδραση Της Δικλοφενάκης Και Σιμβαστατίνης Στην Επούλωση Οστικών Ελλειμμάτων

Georgopoulou-Karanikola¹,

Γεωργοπούλου-Καρανικόλα²

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Στην ιστολογική µελέτη των οστικών και λοιπών δειγµάτων καθοριστική ήταν η συµβολή της κ. Αγγελικής Χέβα, Α΄ επιµελήτρια του Παθολογοανατοµικού Εργαστηρίου του Νοσοκοµείου Παπανικολάου και επιστηµονική συνεργάτιδα του εργαστηρίου Παθολογικής Ανατοµικής της Ιατρικής Σχολής του Α.Π.Θ., η οποία µε βοήθησε ουσιαστικά στη µελέτη και κατανόηση των ιστολογικών δειγµάτων. Την ευχαριστώ θερµά.Τέλος, ένα µεγάλο ευχαριστώ θα ήθελα να εκφράσω στους φίλους και την οικογένειά µου, που µε πολλή αγάπη µε ενθάρρυναν να ολοκληρώσω αυτή µου την προσπάθεια. Ο σύζυγός µου Γιάννης και η κόρη µου Ιωάννα είναι αυτοί που επέδειξαν τη µεγαλύτερη υποµονή αλλά παράλληλα µε τροφοδότησαν µε δύναµη για την ολοκλήρωση της διδακτορικής διατριβής.

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Effect of anti-inflammatory agents on the integration of autogenous bone graft and bovine bone devitalized matrix in rats

Abstract: Diclofenac sodium and meloxicam delayed bone graft repair and dexamethasone did not interfere in it.

Cited by 11 publications

References 15 publications

Alpha-tricalcium phosphate cement in the reconstruction of bone defects in rats

Alpha-tricalcium phosphate cement in the reconstruction of bone defects in rats

Avaliação histomorfométrica da biocompatibilidade do enxerto bovino misto (OrthoGen®) em tecido subcutâneo e o potencial osteogênico em defeito ósseo craniano

Η Επίδραση Της Δικλοφενάκης Και Σιμβαστατίνης Στην Επούλωση Οστικών Ελλειμμάτων

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