Alpha-tricalcium phosphate cement in the reconstruction of bone defects in rats

Abstract

Purpose:To evaluate the ability of a mixture of α-TCP and autogenous bone (AB) vs. α-TCP alone and AB alone to promote new bone formation and tissue repair in bone defects. Methods: Bone defects surgically created in 15 male Wistar rats were divided into four groups: Group I (AB), Group II (α-TCP), Group III (α-TCP+AB assessed by light microscopy), and Group IV (α-TCP+AB assessed by scanning electron microscopy). Bone repair findings were assessed at 30, 60, and 120 days postoperatively. Results: The histological findings obtained in Groups I (p=0.459), II (p=0.368), and III (p=0.459) and at 30 days (p=0.717), 60 days (p=0.717), and 120 days (p=0.779) did not show statistically significant differences. Scanning electron microscopy revealed direct contact between the α-TCP+AB implant and the bone tissue at 120 days. Conclusion: The α-TCP implant is effective alternative bone substitutes for the treatment of critical size bone defects. Key words: Bone Substitutes. Bone Transplantation. Biocompatible Materials. Rats. RESUMOObjetivo: Avaliar a capacidade da mistura de α-TCP e osso autógeno (OA) Vs somente α-TCP e somente OA em promover a neoformação óssea e reparo tecidual em defeitos ósseos. Métodos: Foram criados cirurgicamente defeitos ósseos em 15 ratos Wistar machos, distribuídos em quatro grupos: Grupo I (OA), Grupo II (α-TCP), Grupo III (α-TCP + OA avaliado por microscopia de luz) e Grupo IV (α-TCP + OA avaliado por microscopia eletrônica de varredura). Os resultados da reparação óssea foram avaliados em 30, 60 e 120 dias. Resultados: os achados histológicos obtidos nos grupos I (p = 0,459), II (p = 0,368) e III (p = 0,459) e aos 30 dias (p = 0,717), 60 dias (p = 0,717), e 120 dias (p = 0,779) não apresentaram diferenças estatisticamente significativas. A análise por microscopia eletrônica de varredura revelou contacto direto entre o implante de α-TCP + AO e o tecido ósseo aos 120 dias. Conclusão: O implante de α-TCP é alternativa eficaz como substitutos ósseos para o tratamento de defeitos ósseos de tamanho crítico. Descritores: Substitutos Ósseos. Enxerto Ósseo. Materiais Biocompatíveis. Ratos. -Acta Cirúrgica Brasileira -Vol. 26 (2) 2011Júnior ANS et al.

Is bone transplantation the gold standard for repair of alveolar bone defects?

“…14 Schmitt et al 37 have also demonstrated their capacity to regenerate bone in a critical-sized bone defect. Pinto et al 11 compared the capacity of α-TCP free of cells and autogenous bone to repair bone defects in the femurs of animal models and showed a similar regenerative capacity between the two. The results of Mokbel et al 14 and Pinto et al 11 corroborated ours, indicating (1) low bone volume in the bone defects by using the bovine bone mineral free of cells and (2) high bone volume in the bone defects by using α-TCP free of cells.…”

“…Pinto et al 11 compared the capacity of α-TCP free of cells and autogenous bone to repair bone defects in the femurs of animal models and showed a similar regenerative capacity between the two. The results of Mokbel et al 14 and Pinto et al 11 corroborated ours, indicating (1) low bone volume in the bone defects by using the bovine bone mineral free of cells and (2) high bone volume in the bone defects by using α-TCP free of cells. Thus, this osteoconductive capacity associated with the favorable chemical and physical properties makes them a potential matrix to transport and deliver multipotent adult stem cells in bone defects.…”

“…Calcium phosphate matrices have been used in animal models to replace bone in critical-sized defects. 10,11 The main representatives of this group are α-tricalcium phosphate (TCP) and β-TCP. 12 Although they have similar chemical structures (α-Ca 3 (PO 4 ) 2 and β-Ca 3 (PO 4 ) 2 , respectively), they differ in their crystalline structure, 13 namely, the solubility of α-TCP is higher than β-TCP.…”

Análise da neoformação óssea em transplantes de osso autólogo, osso bovino mineral e tricálcio fosfato com e sem o emprego de células-tronco mesenquimais humanas no reparo de falhas ósseas alveolares por meio de histomorfometria e imagens

“…42,48 Os biomateriais cerâmicos e osso bovino mineral são considerados substitutos ósseos e frequentemente utilizados na bioengenharia de tecidos para carrear células e moléculas indutoras. [49][50][51][52][53][54] A hidroxiapatita 55,56 , o α-tricálcio fosfato (α-TCP) [57][58][59] e o β-tricálcio fosfato (β-TCP) 60 …”