Effect of androgen substrates on the steroidogenic pattern of cumulus cells: Correlation with cumulus culture morphology

Bar‐Ami, Shalom; Regev, Avital; Gitay-Goren, Hela

doi:10.1007/bf02765828

Cited by 4 publications

(1 citation statement)

References 33 publications

(45 reference statements)

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“…aspiration also may be involved in an increased capacity to utilize the exogenously provided pregnenolone for P 4 biosynthesis. Actually, the maximal serum P 4 The retrieved COCs are not synchronized to the same degree of maturation (5,12,19). The possibility level during the luteal phase of the menstrual cycle is seen within the 6-8 days after ovulation.…”

Section: Discussionmentioning

confidence: 99%

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Bar‐Ami

Gitay-Goren

2000

Journal of Assisted Reproduction and Genetics

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Section: Discussionmentioning

confidence: 99%

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Bar‐Ami

Gitay-Goren

2000

Journal of Assisted Reproduction and Genetics

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Immunolocalization of androgen receptor and steroidogenic enzymes in cumuli oophori of pre- and post-ovulatory rats

et al. 2010

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Effects of testosterone and prolactin on steroidogenesis in post-ovulatory cumuli oophori and on in vitro oocyte fertilisation in the rat

Starowicz

Galas

Duda

et al. 2017

Reprod. Fertil. Dev.

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The main objective of these studies was to determine the in vitro effects of prolactin (PRL) and testosterone (T) on steroidogenic function in post-ovulatory cumuli oophori containing unfertilised (ufCOCs) or fertilised (fCOCs) oocytes and to determine the differences between ufCOCs and fCOCs. In vivo, progesterone (P4) content was distinctly higher in isolated ampullae containing ufCOCs than in those containing fCOCs. Moreover, the expression of androgen (ARs) and prolactin (PRL-Rs) receptors was distinctly higher in ufCOCs than in fCOCs. Also, in vitro P4 profiles were generally higher in incubated ufCOCs, which had very high secretion rates of this steroid, especially after treatment with PRL+T. Testosterone significantly increased P4 levels only in incubated fCOCs, while the anti-androgen dihydroxyflutamide (2-Hf) markedly decreased P4 levels in both ufCOCs and fCOCs. Among post-incubation ufCOCs fertilised in vitro, the highest fertilisation rate was observed for oocytes in ufCOCs exposed to PRL+T, while those incubated with 2-Hf or T+2-Hf were not fertilisable. These studies establish differences in steroidogenic function and expression of ARs and PRL-Rs between post-ovulatory ufCOCs and fCOCs, with higher concentrations of P4 being observed in the microenvironment of ufCOCs. PRL+T stimulated P4 production by ufCOCs and increased in vitro fertilisation rate.

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Effect of androgen substrates on the steroidogenic pattern of cumulus cells: Correlation with cumulus culture morphology

Abstract: 10 -7 M dehydroepiandrosterone, 4-androstane-3, 17-dione, or

Cited by 4 publications

References 33 publications

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Immunolocalization of androgen receptor and steroidogenic enzymes in cumuli oophori of pre- and post-ovulatory rats

Effects of testosterone and prolactin on steroidogenesis in post-ovulatory cumuli oophori and on in vitro oocyte fertilisation in the rat

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