Effects of testosterone and prolactin on steroidogenesis in post-ovulatory cumuli oophori and on in vitro oocyte fertilisation in the rat

Starowicz, Agnieszka; Galas, Jerzy; Duda, Małgorzata; Tabarowski, Zbigniew; Szołtys, Maria

doi:10.1071/rd15050

Cited by 5 publications

(5 citation statements)

References 69 publications

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“…Some studies indicate that T and PRL play a signi cant role in the formation of a zygote. The expression of post-ovulatory cumuli oophori containing unfertilized eggs is higher than that containing fertilized eggs, which is consistent with our results [30]. The group involved in our study focused on women of reproductive age between 20 and 40 years old, and the age span is relatively large.…”

Section: Disscussionsupporting

confidence: 92%

Differences in sex hormones and brain morphology between females with offspring and nulliparous

Huo,

Yu,

Sun

et al. 2023

Preprint

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Background This study aims to investigate differences in sex hormone levels and brain morphology among women with offspring and nulliparous to explore the changes that the reproduction induces. Methods A total of 66 females of reproductive age range 20–40 years were enrolled, including 35 offspring women, and 31 nulliparous. Participants’ sex hormone levels were assessed. T1 structural images were obtained using a 3.0 Tesla MRI scanner, and voxel-based morphometry (VBM) was implemented to investigate gray matter changes between the two groups and extract brain volume including gray matter volume (GMV), white matter volume (WMV), cerebrospinal fluid (CSF). Results Analysis of sex hormones revealed no significant differences in E2 (z = − 0.28,p = 0.782), LH (z = − 0.62,p = 0.537), and P (z = − 1.34,p = 0.181), whereas significant differences were found in FSH (z = 3.86,p < 0.001), T (z = − 3.92,p < 0.001), and PRL (z = − 3.27, p = 0.018). Differences in brain volumes, including GMV (t = − 3.53,p = 0.001), CSF (t = − 2.39,p = 0.020), were observed. VBM analysis showed that compared with nulliparous women, those with offspring exhibited decreased cortical areas in the left superior frontal gyrus and right putamen, but no increased cortical areas were found. Conclusion Females of reproductive age in the 20–40 years group, compared with nulliparous female, sex hormones of female with offspring changed, and the volume of the cerebral cortex decreased, which indicated that females were developing in the direction of functional decline. Further research should explore the mechanism underlying these discrepancies, and their potential consequences for female health.

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Section: Disscussionsupporting

confidence: 92%

Differences in sex hormones and brain morphology between females with offspring and nulliparous

Huo,

Yu,

Sun

et al. 2023

Preprint

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“…This may indicate that flutamide affects expression of genes involved in oocyte fertilization in the pig. Such notion is supported by meiotic abnormalities and fertilization failure observed in rat oocytes in vitro exposed to 2-hydroxyflutamide [57]. However, further research is necessary to uncover the nature of molecular processes underlying the association between neonatal anti-androgen exposure and oocyte fertilizing ability in pigs.…”

Section: Discussionmentioning

confidence: 99%

Flutamide-induced alterations in transcriptional profiling of neonatal porcine ovaries

Knapczyk-Stwora

Nynca

Ciereszko

et al. 2019

J Animal Sci Biotechnol

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Background Androgens are involved in the regulation of ovarian development during fetal/neonatal life. Environmental chemicals displaying anti-androgenic activities may affect multiple signal transduction pathways by blocking endogenous androgen action. The aim of the current study was to examine effects of the anti-androgen flutamide on the expression of coding transcripts and long non-coding RNAs (lncRNAs) in neonatal porcine ovaries. By employing RNA-Seq technology we aimed to extend our understanding of the role of androgens in neonatal folliculogenesis and examine the impact of the anti-androgen flutamide on ovarian function. Method Piglets were subcutaneously injected with flutamide (50 mg/kg BW) or corn oil (controls) between postnatal days 1 and 10 ( n = 3/group). Ovaries were excised from the 11-day-old piglets and total cellular RNAs were isolated and sequenced. Results Flutamide-treated piglet ovaries showed 280 differentially expressed genes (DEGs; P -adjusted < 0.05 and log 2 fold change ≥1.0) and 98 differentially expressed lncRNAs (DELs; P -adjusted < 0.05 and log 2 FC ≥ 1.0). The DEGs were assigned to GO term, covering biological processes, molecular functions and cellular components, which linked the DEGs to functions associated with cellular transport, cell divisions and cytoskeleton. In addition, STRING software demonstrated strongest interactions between genes related to cell proliferation. Correlations between DEGs and DELs were also found, revealing that a majority of the genes targeted by the flutamide-affected lncRNAs were associated with intracellular transport and cell division. Conclusions Our results suggest that neonatal exposure of pigs to flutamide alters the expression of genes involved in ovarian cell proliferation, ovarian steroidogenesis and oocyte fertilization, which in turn may affect female reproduction in adult life. Electronic supplementary material The online version of this article (10.1186/s40104-019-0340-y) contains supplementary material, which is available to authorized users.

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“…Control and delor-treated Leydig cell culture media (100 μl) were collected for determination of sex steroid content (testosterone and estradiol, respectively) using the radioimmunological technique described elsewhere (Dufau et al 1972 ; Hotchkiss et al 1971 ; Kotula-Balak et al 2011 , 2013 ; Starowicz et al 2015 ). Testosterone levels were assessed using [1,2,6,7- 3 H]-testosterone (specific activity 110 Ci/mmol; American Radiolabeled Chemicals) as a tracer and rabbit antibody against testosterone-3-0-CMO:BSA (a gift from Dr. B. Ričařova, Institute of Radiology, Czech Academy of Sciences, Prague, Czech Republic).…”

Section: Methodsmentioning

confidence: 99%

Chlorinated biphenyls effect on estrogen-related receptor expression, steroid secretion, mitochondria ultrastructure but not on mitochondrial membrane potential in Leydig cells

et al. 2017

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To characterize polychlorinated biphenyls (PCBs) action on Leydig cells, PCBs congeners, low-chlorinated (delor 103; d103) and high-chlorinated ones (delor 106; d106) were selected. The cells were treated according to PCBs dose (d103 or d106 0.2 ng/ml in low doses:, or 2 ng/ml in high doses) and type (d103 + d106 in low doses or 103 + 106 in high doses). After 24 h treatment with PCBs, a distinct increase in estrogen-related receptors (ERRs type α, β and γ) expression was revealed. However, the dose- and type-dependent PCBs effect was mostly exerted on ERRα expression. A similar increase in ERRs expression was demonstrated by estradiol but not testosterone, which was without an effect on ERRs. PCBs caused no decrease in the membrane potential status of Leydig cells (either in dose or type schedule) but had severe effects on the mitochondria number and structure. Moreover, PCBs markedly increased calcium (Ca2+) concentration and sex steroid secretion (both androgens and estrogens were elevated). These findings suggest a similar estrogenic action of PCBs congeners (d103 and d106) on Leydig cell function. We report dose- and type-specific effects of PCBs only on Leydig cell ERRs expression. Both delors showed common effects on the mitochondria ultrastructural and functional status. Based on our results, ERRα seems to be the most sensitive to hormonal modulation. The increases in Ca2+ and sex steroid secretion may be due to the activation of ERRs by PCBs binding and/or direct effect of PCBs on ERRs mRNA/protein expression. Nevertheless, to confirm the existence of possible relationships between ERRs signaling (including PCBs as ligands) and mitochondria function in Leydig cells, further intensive studies are needed.

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Effects of testosterone and prolactin on steroidogenesis in post-ovulatory cumuli oophori and on in vitro oocyte fertilisation in the rat

Cited by 5 publications

References 69 publications

Differences in sex hormones and brain morphology between females with offspring and nulliparous

Differences in sex hormones and brain morphology between females with offspring and nulliparous

Flutamide-induced alterations in transcriptional profiling of neonatal porcine ovaries

Chlorinated biphenyls effect on estrogen-related receptor expression, steroid secretion, mitochondria ultrastructure but not on mitochondrial membrane potential in Leydig cells

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