Immunolocalization of androgen receptor and steroidogenic enzymes in cumuli oophori of pre- and post-ovulatory rats

Szołtys, Maria; Słomczyńska, Maria; Knapczyk-Stwora, Katarzyna; Durlej, Małgorzata; Lechowska, Agnieszka; Starowicz, Agnieszka

doi:10.1016/j.acthis.2009.06.003

Cited by 7 publications

(5 citation statements)

References 37 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…The compartmentalized expression of AR with higher expression in CCs than in MGCs is in accordance with the previous human study (Koks et al, 2010) and a study on immunolocalization of AR found it to be mainly located in the COC and not in MGCs in pre-ovulatory follicles in mice (Szoltys et al, 2010). The top cell-cell signalling network enriched in the differentially expressed genes showed AR placed centrally in the network, indicating a sustained function signalling through AR in the CCs in the pre-ovulatory follicle close to ovulation.…”

Section: Steroid Hormone Receptors and Other Nuclear Receptorssupporting

confidence: 92%

“…The CCs in the present study represent the outer part of the cumulus in the COC, isolated immediately after OPU, whereas the whole cumulus were isolated enzymatically after 4 h of culture (Koks et al, 2010). It has been .. ........................................................................................................................................................................................... shown in COCs from the rat that differences exist both at the level of expression and the protein of some genes between cells in proximity to the oocyte compared with peripherally located cells in the cumulus oophorus both pre-and post-ovulation (Szoltys et al, 2010). Likewise, culture is known to influence the expression levels in MGCs (Robert et al, 2003;Yamashita et al, 2010).…”

Section: Extracellular Matrix: Formation and Remodellingmentioning

confidence: 99%

See 1 more Smart Citation

Specific genes are selectively expressed between cumulus and granulosa cells from individual human pre-ovulatory follicles

Grøndahl

Andersen

Bogstad

et al. 2012

MHR: Basic Science of Reproductive Medicine

View full text Add to dashboard Cite

During folliculogenesis, the granulosa cells differentiate into two cell types: cumulus cells (CCs) and mural granulosa cells (MGCs). The objective of the study was to generate and compare the transcriptomes of MGCs and CCs from the pre-ovulatory follicle to characterize the detailed profile of the two cell populations shortly before ovulation. Twenty-one IVF/ICSI patients undergoing controlled ovarian stimulation (COS) donated CCs and MGCs from individual follicles containing metaphase II oocytes. Cells were prepared immediately after recovery and mRNA was isolated for whole-genome gene expression analysis and reverse transcriptase-polymerase chain reactions. Paired (within the individual follicle) comparisons between the CC and MGC expression profiles were performed and corrected for multiple comparisons. A total of 1562 genes were differentially expressed by >2-fold (P < 0.01) in the two cell types. Of these, 156 genes were >8-fold changed and represented specialized cellular functional categories such as inflammatory response, extracellular matrix and cell-cell communication, whereas the 1406 genes were 2-8-fold changed and represented functional categories such as proliferation and lipid metabolism. Transcripts not previously linked to the follicle were found to be differentially expressed between CCs and MGCs, suggesting specialized function in these compartments, e.g. pepsinogen A was selectively expressed in MGCs, whereas ryanodine receptor-2 (RYR2) was selectively expressed in CCs. Positive correlations were present between expression levels of RYR2 and the amphiregulin and gap-junction proteins. In conclusion, the transcriptomes of corresponding CCs and MGCs from individual pre-ovulatory follicles clearly revealed two distinct cell types. New as well as known genes representing specific cell functions close to ovulation were highlighted.

show abstract

Section: Steroid Hormone Receptors and Other Nuclear Receptorssupporting

confidence: 92%

Section: Extracellular Matrix: Formation and Remodellingmentioning

confidence: 99%

Specific genes are selectively expressed between cumulus and granulosa cells from individual human pre-ovulatory follicles

Grøndahl

Andersen

Bogstad

et al. 2012

MHR: Basic Science of Reproductive Medicine

View full text Add to dashboard Cite

show abstract

“…Granulosa cells are the primary component of the follicular microenvironment and perform various functions during the ovulation period, such as steroidogenesis, angiogenesis, oocyte meiotic resumption and so on [ 15 , 16 ]. There was no obvious distinction between CCs and MGCs in the GCs in the preovulatory follicles in our data ( Figure S2D ), which was not only consistent with a previous study in rats but also the single cell results in small antral follicles in humans [ 46 , 47 ]. As expected, GCs in preovulatory follicles gathered in several different functional clusters.…”

Section: Resultssupporting

confidence: 93%

“…Grondahl et al [ 15 ] compared the transcriptomes of MGCs and CCs from preovulatory follicles with microarrays, which could not fully reveal the different functions within GCs. The distinctions between MGCs and CCs were not significant in preovulatory follicles, suggesting MGCs and CCs performed varieties of functions and may share some molecular functions, consistent with previous studies on ovarian follicles [ 46 , 47 ]. Here we identified nine different functional clusters in GCs.…”

Section: Discussionsupporting

confidence: 90%

Single-Cell Sequencing Reveals an Intrinsic Heterogeneity of the Preovulatory Follicular Microenvironment

Zhu

Zheng

et al. 2022

Biomolecules

View full text Add to dashboard Cite

The follicular microenvironment, including intra-follicular granulosa cells (GCs), is responsible for oocyte maturation and subsequent ovulation. However, the functions of GCs and cellular components of the follicular microenvironment in preovulatory follicles have not been extensively explored. Here, we surveyed the single-cell transcriptome of the follicular microenvironment around MII oocytes in six human preovulatory follicles in in vitro fertilization. There were six different cell types in the preovulatory follicles, including GCs and various immune cells. In GCs, we identified nine different functional clusters with different functional transcriptomic profiles, including specific clusters involved in inflammatory responses and adhesive function. Follicular macrophages are involved in immune responses, extracellular matrix remoulding and assist GCs in promoting the oocyte meiotic resumption. Interestingly, we observed that the specific terminal state subcluster of GCs with high levels of adhesive-related molecules should result in macrophage recruitment and residence, further contributing to an obvious heterogeneity of the immune cell proportion in preovulatory follicles from different patients. Our results provide a comprehensive understanding of the transcriptomic landscape of the preovulatory follicular microenvironment at the single-cell level. It provides valuable insights into understanding the regulation of the oocyte maturation and ovulation process, offering potential clues for the diagnosis and treatment of oocyte-maturation-related and ovulation-related diseases.

show abstract

“…Immunohistochemistry was performed as previously described (Szołtys et al, ). Briefly, sections were deparaffinized, rehydrated, and rinsed in water.…”

Section: Methodsmentioning

confidence: 99%

Steroid Concentrations and Immunoexpression of Steroidogenic Enzymes in Ovaries of Aged Bank Voles: Effect of Photoperiod

et al. 2012

Self Cite

View full text Add to dashboard Cite

The main objective of the present study was to establish morphological and steroidogenic changes occurring in the ovaries of senescent bank voles, with respect to the photoperiod of rearing. Obtained results revealed less pronounced changes in the ovaries of females reared in a long photoperiod (LD). Their gonads still possessed some healthy follicles and old corpora lutea (CLs). Senescence-related changes encompassed the presence of abnormal follicles, large regions containing extra-follicular luteinized granulosa cells and numerous clusters of hypertrophied theca/interstitial cells, exhibiting strong expression of 3β-hydroxysteroid dehydrogenase (3β-HSD) and much weaker that of cytochrome P450c17. More pronounced changes were observed in animals reared in short day (SD) conditions and included the presence of only few, usually abnormal follicles and/or remnants of CLs in the surface region, and the isle-like clusters of cells in the ovarian medulla. The clusters were composed of cells generally featuring strong 3β-HSD and/or P450c17 immunoreaction. Steroid content analysis revealed that progesterone dominated in the ovaries of LD bank voles and androgens in SD animals, while estradiol content was very low in both investigated groups. These studies showed for the first time morphological and steroidogenic changes found in the ovaries of senescent bank voles and indicated an important role of length light conditions in the process of reproductive aging.

show abstract

Immunolocalization of androgen receptor and steroidogenic enzymes in cumuli oophori of pre- and post-ovulatory rats

Cited by 7 publications

References 37 publications

Specific genes are selectively expressed between cumulus and granulosa cells from individual human pre-ovulatory follicles

Specific genes are selectively expressed between cumulus and granulosa cells from individual human pre-ovulatory follicles

Single-Cell Sequencing Reveals an Intrinsic Heterogeneity of the Preovulatory Follicular Microenvironment

Steroid Concentrations and Immunoexpression of Steroidogenic Enzymes in Ovaries of Aged Bank Voles: Effect of Photoperiod

Contact Info

Product

Resources

About