Effect of an extract of the mushroom Agaricus blazei Murill on expression of adhesion molecules and production of reactive oxygen species in monocytes and granulocytes in human whole blood ex vivo

Abstract

We have reported that an extract of the edible officinal mushroom Agaricus blazei Murill (AbM) stimulates synthesis of pro-inflammatory cytokines in human monocytes and vein endothelial cells in vitro and reduces the extent of lethal septicemia in mice with bacterial peritonitis. In the present study on human monocytes and granulocytes in whole blood ex vivo, we studied the dynamic changes of cell adhesion molecules (CD11b, CD62L) and the production of reactive oxygen species (ROS) after stimulation with AbM. The presence of AbM resulted in a similarly increased expression of CD11b in monocytes and granulocytes, although at a lower AbM concentration in monocytes (0.5%) than in granulocytes (2%). Furthermore, there was an AbM-mediated decrease in CD62L expression mirroring the effect on CD11b expression regarding magnitude and dose response. The intracellular production of ROS increased slightly but significantly in granulocytes, but not in monocytes stimulated with AbM. The results suggested that the major effect of AbM on monocytes and granulocytes was the upregulation of CD11b expression, thereby increasing both the phagocytic potential and the ablility to induce diapedesis into inflammatory foci. The rich beta-glucan content of AbM could play a crucial role in this immune response.

Stimulation of human monocytic cells by the medicinal mushroom Agaricus blazei Murill induces expression of cell surface markers associated with activation and antigen presentation

“…Interestingly, stimulation with this mushroom extract induced a similar upregulation of CD69 (associated with activation) and CD86 (associated with antigen-presentation) as did stimulation with E.coli LPS. These findings are in line with the results from a previous investigation, where stimulation with AndoSan TM on MDCC was found to increase production of cytokines in a similar manner as stimulation with LPS, and, in some cases, in even higher quantities than did stimulation with LPS [34]. The β (or leukocyte)-integrins, i.e.…”

Effect of Hot Water Extract from Agaricus Blazei Murill on Chemotaxis of Neutrophils

“…We evaluated ROS production of neutrophils using DCFH-DA and HE by FACS [26] and found that H 2 O 2 and production were significantly in- creased by exposure to ABM extract compared with control. These results are similar to a report that ROS production in granulocytes of human whole blood was increased by ABM [27].…”

Effect of AndoSan™on expression of adhesion molecules and production of reactive oxygen species in human monocytes and granulocytesin vivo

“…Ex vivo studies [18] in whole blood demonstrated that AbM decreased the expression of adhesion molecule CD62L (L-selectin) and increased the expression of the b 2 integrin CD11b on monocytes and granulocytes. These molecules are crucial for respectively rolling and adherence to endothelial cells [19,20], and prerequisites for the ultimate transcapillary diapedesis of leukocytes to inflammatory foci in order to attack microorganisms.…”