Effect of Hot Water Extract from Agaricus Blazei Murill on Chemotaxis of Neutrophils

Miyagawa, Matsuo; Hirono, Yuriko; Kawazoe, Ayaka; Shigeyoshi, Eri; Nose, Masahito; Sakura, Masaaki; Pinkerton, Kent E.; Takeuchi, Minoru

doi:10.4236/jcdsa.2013.31a003

Cited by 2 publications

(1 citation statement)

References 22 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…A phagocytosis assay has been proposed as an indicator for assessing protective mechanisms [51]. In guinea pigs, Miyagawa et al reported that significantly higher phagocytic activity was seen in neutrophils treated with hot water extract from fruiting bodies of A. blazei compared with control [52]. Additionally, the hot water extract of the A. blazei mycelium significantly increased the activation of dendritic cells [53].…”

Section: Discussionmentioning

confidence: 99%

Dietary Agaricus blazei Spent Substrate Improves Disease Resistance of Nile Tilapia (Oreochromis niloticus) against Streptococcus agalactiae In Vivo

Lee

Tseng

et al. 2022

JMSE

View full text Add to dashboard Cite

This study evaluated the effects of the feeding of spent mushroom substrate from Agaricus blazei on Nile tilapia (Oreochromis niloticus). The safety of 0–1000 μg/mL A. blazei spent substrate water extract (ABSSE) was demonstrated in the primary hepatic and splenic macrophages and the THK cell line (a cell line with characteristics of melanomacrophages) using a cytotoxicity assay. Here, 10 μg/mL of crude ABSSE promoted the phagocytic activity of macrophages and THK cells. Stimulating ABSSE-primed THK cells with lipopolysaccharides or peptidoglycan resulted in higher expression levels of four cytokine genes (e.g., interleukinz (IL)-1β, IL-12b, IL-8 and tumor necrosis factor α (TNFα)) and one cytokine gene (TNFα), respectively. An in vitro bacterial growth inhibition assay demonstrated that ABSSE could inhibit the growth of Streptococcus agalactiae. In the first feeding trial, Nile tilapia were fed with experimental feed containing 0, 1, or 5% of A. blazei spent substrate (ABSS) for seven and fourteen days followed by bacterial challenge assay. The best result was obtained when Nile tilapia were continuously fed for seven days on a diet containing 1% ABSS, with the survival rate being higher than in groups with 0% and 5% ABSS after challenge with S. agalactiae. In the second trial, fish were fed diets supplemented with 0% or 1% ABSS for seven days, and then all the groups were given the control feed for several days prior to bacterial challenge in order to investigate the duration of the protective effect provided by ABSS. The results showed that the protective effects were sustained at day 7 after the feed was switched. Overall, spent mushroom substrate from A. blazei is a cost-effective feed additive for Nile tilapia that protects fish from S. agalactiae infection.

show abstract

Section: Discussionmentioning

confidence: 99%