Effect of an antifungal denture liner on the saliva yeast count in patients with denture stomatitis: a pilot study

Abstract

Although in vitro studies on the release of antifungal agents from tissue conditioners have been done, no in vivo research on the topic could be found. The purpose of this study was to determine the in vivo effect of an antifungal agent released from a tissue conditioner on the salivary yeast count. Forty edentulous patients with denture stomatitis caused by Candida albicans were divided in two groups. Group 1 (control) was treated with a tissue conditioner only. Group 2 was treated with a tissue conditioner incorporating 500,000 U nystatin. Oral rinses were performed by both groups before treatment and every second day during treatment for a period of 14 days. Total yeast counts of the oral rinses were performed and the averages and standard deviations for both groups calculated and logarithm-transformed data of the counts over time were statistically analysed using the Wilcoxon signed-rank test. The average oral rinse yeast count of the control group decreased up to day 4. Thereafter, the count increased till the end of the test period. At day 14, the oral rinse yeast level was higher than the pre-treatment level. The average yeast count of the test group decreased up to day 7. Thereafter, the count increased but remained significantly lower (P=0.01) than the control group and did not return to its pre-treatment level. A nystatin-containing short-term denture liner significantly decreases the salivary yeast count of patients with denture stomatitis compared with a liner without nystatin.

Effect of antimicrobial agents incorporated into resilient denture relines on the Candida albicans biofilm

“…Also, 14‐day interval is the recommended period for treatment of denture stomatitis with conventional topical antifungals such as nystatin (Silva et al , ). The effectiveness demonstrated by nystatin in the resilient materials tested in this study corroborates the reports of other authors (Thomas and Nutt, ; Quinn, ; Schneid, ; Geerts et al , ; Falah‐Tafti et al , ).…”

“…(Douglas and Walker, ; Geerts et al , ). The rare detection of fungal strains resistant to nystatin and their accessible cost are other advantages of this drug compared with other antifungals (Geerts et al , ). Similar results to those obtained in this study by addition of nystatin to a tissue conditioner were previously obtained (Truhlar et al , ).…”

“…The MICs were defined as minimal drug concentrations capable of inhibiting fungal growth during the evaluation period (14 days) in percentages ≥90 (Isham and Ghannoum, ; Amran et al , ). The maximum 14‐day period was selected based on previous studies that evaluated temporary denture liners modified by antimicrobials as an alternative to the conventional antifungal therapy for denture stomatitis (Quinn, ; Schneid, ; Truhlar et al , ; Chow et al , ; Geerts et al , ).…”

In vitroantimicrobial effect of the tissue conditioner containing silver nanoparticles

“…The results of the present study implicate that Ag° added tissue conditioner might act like latent antimicrobial material and it could provide the additional benefit of antimicrobial effect even if dentures are worn at night, therefore, could be used as an one of the alternative therapy for denture stomatitis resistant to conventional treatment or geriatric denture bearing patients under medically compromised status 34. However, further studies are still required to clarify the optimal concentration of Ag°, regarding the silver content, the possible disruption of oral microflora and toxic effects of silver with excess Ag°and mechanical stability should be also considered for the proper and safe clinical application 35…”

“Nature cures:” An alternative herbal formulation as a denture cleanser

“…[34] Denture wearing is a predisposing factor for oral colonization by Candida and its prevalence can increase from 60% to 100%. [35]…”