Effect of Amino Acids on Alcohol Intake in Stroke-Prone Spontaneously Hypertensive Rats.

Abstract

SummaryExamination was made of the effects of amino acids on alcohol intake in stroke-prone spontaneously hypertensive rats (SHRSP) . The animals were divided into two groups according to dietary protein level, normal (15%) and low (5%). The two groups were further divided into two groups according to whether they selected the ethanol solution containing amino acids (non-amino acid group and amino acid group). The 5% ethanol solutions with and without various amino acids were prepared in a water-supplying tube to which the animals had free access for 40 days. The 5% ethanol solution intake in rats fed the normal protein diet was higher than that of the low protein group. Regardless of dietary protein level, 5% ethanol solution intake increased in the amino acid group. Intake of the 5% ethanol solution containing 100mM L-proline, 100mM L-lysine, and 100mM L-threonine was large. For the amino acid group of rats fed normal protein and low protein, plasma glutamate oxalacetate transaminase (GOT) activity was significantly reduced. It is suggested that the alcohol intake may increase by adding amino acid to the alcohol solution. Key Words amino acids, alcohol intake, stroke-prone spontaneously hypertensive rats (SHRSP), dietary protein level, glutamate oxalacetate transaminase (GOT)The relationship between taste preference and nutritional state should be investigated. The preference for alcohol was previously found to depend not only on genetic factors, but nutritional status as well. That is, preference for alcohol was higher in spontaneously hypertensive rats (SHR) on a high protein diet than in SHR fed a low protein diet (1), The disappearance of ethanol in blood of rats fed the high protein diet was earlier than that of rats fed the low protein diet following the oral administration of ethanol (1). The efficiency of alcohol metabolism may 55

Effect of Long-Term 'Corn Pepffde' Ingestion on Alcohol Metabolism in Stroke-Prone Spontaneously Hypertensive Rats with Alcohol Loading.

“…The increase in body weight was probably caused by the preference to drink more water containing proline during ethanol loading. This result was in agreement with a previous report by Yang et al (4). Concerning changes in the plasma amino-acid concentration, glycine, histidine and taurine levels increased in rats subjected to long-term ethanol loading as compared to those without ethanol loading.…”

“…When SHR-SP were fed normal or low-protein diets, the long-term supplement of amino acids suppressed an increase in the plasma GOT activity but not in the plasma GPT activity (4). Nevertheless, the ingestion of proline and lysine by SHR-SP after long-term ethanol loading failed to decrease neither GOT nor GPT activiy (8).…”

“…It seems likely that the deficiency of these essential amino acids affects protein synthesis and reduces alcohol-metabolizing enzymes. In this connection, Yang et al showed that the preference for alcohol and its intake in SHR-SP were increased by the administration of an alcoholic solution containing proline, lysine and threonine (4). It is therefore feasible that the alcohol metabolism is elevated by the supply of such amino acids in addition to alcohol.…”

The Effects of Corn Peptide Ingestion on Facilitating Alcohol Metabolism in Healthy Men

“…We reported that administration of proline and lysine to SHR-SP increased in preference for alcohol 3 ) and accelerated alcohol metabolism. 4 ) However, no studies have been done to investigate the mechanism of the effect on facilitating alcohol metabolism by which the intake of oligopeptide, rich in these amino acids, reduces the increase in blood ethanol level in rats or humans.…”

Comparative Study of Alcohol Metabolism in Stroke-Prone Spontaneously Hypertensive Rats and Wistar-Kyoto Rats Fed Normal or Low Levels of Dietary Protein.

“…(19)(20)(21)(22). We had previously shown that the intake of alcohol can be increased in SHRSP by adding amino acids to ethanol solutions and that the intake of proline, lysine, and threonine was considerably larger (23). We also found that proline and lysine can regulate ethanol metabolism (24).…”