Effect of Allicin against Ischemia/Hypoxia‐Induced H9c2 Myoblast Apoptosis via eNOS/NO Pathway‐Mediated Antioxidant Activity

Ma, Lina; Chen, Shangke; Li, Shaochun; Deng, Lijuan; Li, Yikui; Li, Hao

doi:10.1155/2018/3207973

Cited by 23 publications

(18 citation statements)

References 36 publications

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“…Nrf2 is a redox‐sensitive transcription factor that plays a pivotal role in cellular antioxidant defense. Nrf2 trans‐activation promotes the up‐regulation of antioxidant genes responsible for the expression of antioxidant enzymes 30 . This brings to mind that the effects of garlic on TAC may occur through increasing the intracellular antioxidants such as glutathione, uric acid, bilirubin, and by stimulating the expression of antioxidant enzymes 26,31 …”

Section: Discussionmentioning

confidence: 99%

A randomized, double‐blind, placebo‐controlled clinical trial, evaluating the garlic supplement effects on some serum biomarkers of oxidative stress, and quality of life in women with rheumatoid arthritis

2020

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Aim Rheumatoid arthritis (RA), is a prevalent immune‐inflammatory disease, which is associated with disabling pain. Oxidative stress might play a role in RA pathogenesis and outcomes. According to the antioxidant properties of garlic, the current study was performed to evaluate the garlic supplement effects on some serum levels of oxidative stress biomarkers, and quality of life in patients with rheumatoid arthritis. Methods Seventy women with RA participated in this randomized, double‐blind, placebo‐controlled, parallel‐design trial. The patients were randomly divided into two groups, receiving two tablets of either 500 mg garlic or placebo daily for 8 weeks. Serum levels of total antioxidant capacity (TAC) and malondialdehyde (MDA) and quality of life were determined at baseline and end of week 8. A health assessment questionnaire (HAQ) was used to evaluate the quality of life related to health. Results Of 70 patients enrolled in the trial, 62 subjects were included in the final analysis. At the end of the study, there was a significant increase in serum levels of TAC in the garlic group as compared with the placebo group (26.58 ± 77.30 nmol of Trolox equivalent/ml vs 16.11 ± 0.92 nmol of Trolox equivalent/mL; P = .026). In addition, MDA levels were significantly decreased in the intervention group compared with the control group (−0.82 ± 1.99 nmol/mL vs 0.36 ± 2.57 nmol/mL; P = .032). Pain after activity and HAQ scores decreased in the garlic group compared with the placebo (−11.96 ± 13.43 mm vs −0.06 ± 13.41 mm; P < .001, 0.17 ± 20 vs 0.05 ± 0.15; P < .001, respectively). Conclusions The findings suggest that garlic supplementation for 8 weeks resulted in significant improvements in oxidative stress, HAQ in women with RA.

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Section: Discussionmentioning

confidence: 99%

A randomized, double‐blind, placebo‐controlled clinical trial, evaluating the garlic supplement effects on some serum biomarkers of oxidative stress, and quality of life in women with rheumatoid arthritis

2020

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“…Garlic (Allium sativum), a spice belongs to the Alliacae family, and its preparations have been widely recognized as agents for prevention and treatment of CVDs like atherosclerosis, hyperlipidemia, thrombosis, and hypertension [13]. Recent molecular studies have also demonstrated that active ingredients like s-allyl-cysteine sulfoxide (alliin) [14], diallyl trisulfide (DATS) [15], Allicin (2-propene-1-sulfinothioic acid S-2-propenyl ester, diallyl thiosulfinate) [16,17] of garlic protect cardiac tissue through down regulating the expression of some CVD causing apoptotic genes.…”

Section: Introductionmentioning

confidence: 99%

Cardioprotective effect of garlic extract in isoproterenol-induced myocardial infarction in a rat model: assessment of pro-apoptotic caspase-3 gene expression

et al. 2020

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Background Myocardial Infarction (MI), also known as heart attack, is one of the most common cardiovascular diseases. Although certain drugs or mechanical means are used, day by day natural products such as herbs and spices based MI treatment is getting much popularity over the drugs or mechanical means for their pharmacological effects and have low or no side effects. This study was designed to assess the cardio-protective effect of methanolic extract of Bangladeshi multi clove garlic (Allium sativum) cultivar, a highly believed spice having cardioprotective activity, against isoproterenol (ISO) induced MI through cardiac histopathology as well as cardiac apoptotic caspase-3 gene expression study in female Wistar albino rats. Four groups containing 35 rats treated with respective agents like distill water / garlic extract (200 mg/kg-body-weight/day) up to 28 days and normal saline / ISO (100 mg/kg-body-weight/day) on 29th and 30th day were sacrificed (two rats/group/sacrifice) on the day 31, 46 and 61 and collecting the heart, cardiac histology and gene expression analysis were performed. Results ISO induced MI rats pretreated with garlic extract revealed up regulated expression of the cardiac apoptotic caspase-3 gene at the initial stage but finally the expressions gradually getting down regulated along with gradual improving the cardiac damage caused by apoptosis. Furthermore, only garlic extract pretreated rats were found undamaged cardioarchitecture and normal expressions of this gene. Conclusions These findings suggested that garlic extract confers having significant cardioprotective effect and consuming this spice with regular diet may reduce the risk of MI.

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“…Allicin increased SOD and NO release activity, decreased MDA levels, significantly increased eNOS, Nrf2, and HO-1 protein expression and showed protective effect via antioxidant activity mediated by eNOS/NO pathway [25]. Another study found that allicin remarkably attenuated diabetes-induced decrease of NO production and eNOS expression, with a concomitant improvement in endothelial-dependent relaxation.…”

Section: Discussionmentioning

confidence: 99%

Protective Effects of Allicin on ISO-Induced Rat Model of Myocardial Infarction via JNK Signaling Pathway

et al. 2020

Pharmacology

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Objective: This research was aimed to explore protective effects of allicin on rat model of myocardial infarction via JNK signaling pathway. Methods: Rat myocardial ischemia model was established with subcutaneous injection of isoproterenol (ISO). Seventy-five rats were randomly divided into 5 groups (n = 15): sham group, ISO group, low-dose group (1.2 mg/kg/days for 7 days), medium-dose group (1.8 mg/kg/days for 7 days), and high-dose group (3.6 mg/kg/days for 7 days). Routine HE staining and Masson staining were performed to observe myocardial histopathology. The expression of oxidative stress-related indicators, heart tissue apoptosis-related proteins, and JNK and p-JNK proteins were measured for different groups. Results: Compared with the sham group, the T wave value of the ISO group was significantly increased (p < 0.01). When allicin was administered, the T wave values at different time points in all groups were all decreased. Compared with the sham group, the ratio of eNOS, Bcl-2/Bax was significantly decreased, and p-eNOS, iNOS, caspase-3, caspase-9, and Cyt-c were significantly elevated in the ISO group (p < 0.05). After allicin was administered, significant changes in these proteins were observed in the medium- and high-dose groups. There was no significant change in the expression of JNK protein in the ISO group compared with the sham group; however, the expression of eNOS and p-JNK protein were significantly upregulated (p < 0.01) and the expression of p-eNOS and iNOS were significantly downregulated (p < 0.01). When allicin was administered, expression of p-JNK protein was significantly downregulated. Conclusion: Allicin can reduce oxidative stress damage and cardiomyocyte apoptosis in rat model of myocardial infarction and can significantly regulate JNK signaling pathway.

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Effect of Allicin against Ischemia/Hypoxia‐Induced H9c2 Myoblast Apoptosis via eNOS/NO Pathway‐Mediated Antioxidant Activity

Cited by 23 publications

References 36 publications

A randomized, double‐blind, placebo‐controlled clinical trial, evaluating the garlic supplement effects on some serum biomarkers of oxidative stress, and quality of life in women with rheumatoid arthritis

A randomized, double‐blind, placebo‐controlled clinical trial, evaluating the garlic supplement effects on some serum biomarkers of oxidative stress, and quality of life in women with rheumatoid arthritis

Cardioprotective effect of garlic extract in isoproterenol-induced myocardial infarction in a rat model: assessment of pro-apoptotic caspase-3 gene expression

Protective Effects of Allicin on ISO-Induced Rat Model of Myocardial Infarction via JNK Signaling Pathway

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