Effect of agitator configuration on bacterial cellulose productivity in aerated and agitated culture

Kouda, Tohru; Yano, Hisato; Yoshinaga, Fumihiro

doi:10.1016/s0922-338x(97)80144-4

Cited by 101 publications

(73 citation statements)

References 16 publications

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“…For BC production, corn steep liquor with fructose (CSL-Fru) medium was slightly modified as described previously (Kouda et al 1997), and contains the following constituents per liter: 50 g of fructose, 20 mL of CSL (corn steep liquor, Nihon Starch Industry, Kagoshima, Japan), 1.0 g of KH 2 PO 4 , 0.25 g of MgSO 4 Á7H 2 O, 3.3 g of (NH) 2 SO 4 , 3. …”

Section: Mediummentioning

confidence: 99%

Effect of different additives on bacterial cellulose production by Acetobacter xylinum and analysis of material property

2009

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Bacterial cellulose (BC) demonstrates unique properties including high mechanical strength, high crystallinity, and high water retention ability, which make it an useful material in many industries, such as food, paper manufacturing, and pharmaceutical application. In this study, different additives including agar, carboxymethylcellulose (CMC), microcrystalline cellulose, and sodium alginate were added into fermentation medium in agitated culture to enhance BC production by Acetobacter xylinum. The optimal additive was chosen based on the amount of BC produced. The produced BC was analyzed by using X-ray diffraction (XRD), field emission scanning electron microscopy (FESEM), thermogravimetric analysis (TGA), and dynamic mechanical analysis (DMA). Among the evaluated additives, CMC yielded highest BC production (8.2 g/L) compared to the control (1.3 g/L). The results also indicated that CMC-altered BC production increased with CMC addition and reached saturation around 1%. The variation between replicates for all analysis was \5%. From XRD analysis, however, the crystallinity and crystal size decreased as CMC addition increased. FESEM results showed CMC-altered BC produced from agitated culture retained its interweaving property. TGA results demonstrated that CMC-altered BC had about 98% water retention ability, which is higher than BC pellicle produced with static culture. CMC-altered BC also exhibited higher T max compared to control. Finally, DMA results showed that BC from agitated culture loses its mechanical strength in both stress at break and Young's modulus when compared to BC pellicle. This study clearly demonstrated that addition of CMC enhanced BC production and slightly changed its structure.

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Section: Mediummentioning

confidence: 99%

Effect of different additives on bacterial cellulose production by Acetobacter xylinum and analysis of material property

2009

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“…These include fruit juices (Kurosumi et al 2009), sugar cane molasses (Keshk and Sameshima 2006), sweet potato pulp (Shigematsu et al 2005), rotten apples (Gupta et al 2010), and maple syrup (Zeng et al 2011). The second factor is operating conditions such as dissolved oxygen (Kouda et al 1997), temperature, pH of the growth medium (Noro et al 2004), inoculation ratio (Hutchens et al 2007), and inoculum age. Both groups of factors must be at optimized levels to ensure optimum production of microbial cellulose.…”

Section: Introductionmentioning

confidence: 99%

Process Parameters for Fermentation in a Rotary Disc Reactor for Optimum Microbial Cellulose Production using Response Surface Methodology

Zahan¹,

Pa’e²,

Muhamad³

2014

BioResources

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“…Agitated culture, on the other hand, is more suitable for the commercial-scale production as higher production rates can be achieved. So with the aim of improving BC production in agitated culture, the screening of suitable strains, improvement of the producing organisms, medium components and some basic operational parameters about diVerent kinds of reactors, including optimal oxygen supply, volumetric agitation power and so on, have been investigated [7,[11][12][13]. But this method has posed some problems, e.g.…”

Section: Introductionmentioning

confidence: 99%

Effect of addition of sodium alginate on bacterial cellulose production by Acetobacter xylinum

Zhou

Sun

et al. 2007

J Ind Microbiol Biotechnol

141

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Bacterial cellulose (BC) production by Acetobacter xylinum NUST4.1 was carried out in the shake flask and in a stirred-tank reactor by means of adding sodium alginate (NaAlg) into the medium. When 0.04% (w/v) NaAlg was added in the shake flask, BC production reached 6.0 g/l and the terminal yield of the cellulose was 27% of the total sugar initially added, compared with 3.7 g/l and 24% in the control, respectively. The variation between replicates in all determinations was less than 5%. During the cultivation in the stirred-tank reactor, the addition of NaAlg changed the morphology of cellulose from the irregular clumps and fibrous masses entangled in the internals to discrete masses dispersing into the broth, which indicates that NaAlg hinders formation of large clumps of BC, and enhances cellulose yield. Because the structure of cellulose is changed depending on the culture condition such as additives, structural characteristics of BC produced in the NaAlg-free and NaAlg medium are compared using scanning electron microscopy (SEM), fourier transform infrared spectroscopy (FT-IR), X-ray diffraction (XRD). SEM photographs show some differences in reticulated structures and ribbon width and FT-IR spectra indicate that there is the hydrogen bonding interaction between BC and NaAlg, then X-ray diffraction (XRD) analysis reveals that BC produced with NaAlg-added has a lower crystallinity and a smaller crystalline size. The results show that enhanced yields and modification of cellulose structure occur in the presence of NaAlg.

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Effect of agitator configuration on bacterial cellulose productivity in aerated and agitated culture

Cited by 101 publications

References 16 publications

Effect of different additives on bacterial cellulose production by Acetobacter xylinum and analysis of material property

Effect of different additives on bacterial cellulose production by Acetobacter xylinum and analysis of material property

Process Parameters for Fermentation in a Rotary Disc Reactor for Optimum Microbial Cellulose Production using Response Surface Methodology

Effect of addition of sodium alginate on bacterial cellulose production by Acetobacter xylinum

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