Process Parameters for Fermentation in a Rotary Disc Reactor for Optimum Microbial Cellulose Production using Response Surface Methodology

Zahan, Khairul Azly; Pa’e, Norhayati; Muhamad, Ida Idayu

doi:10.15376/biores.9.2.1858-1872

Cited by 25 publications

(15 citation statements)

References 21 publications

(16 reference statements)

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“…However, the pH adjustment had a clear positive effect on volumetric productivity of BNC, BNC yield on initial glucose, and BNC yield on consumed glucose ( Table 2 ). This result agrees with reports that the pH of the medium strongly affects BNC production and that the optimal pH for BNC production is in the range of 4.0–6.0, as this is a favorable pH range for the bacteria [ 20 , 43 ]. An adjustment of pH resulted in a decrease of the DP v of BNC from 6310 to 6080 ( Table 2 ).…”

Section: Resultssupporting

confidence: 92%

Bioconversion of Waste Fiber Sludge to Bacterial Nanocellulose and Use for Reinforcement of CTMP Paper Sheets

Chen

Alriksson³

et al. 2017

Polymers

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Utilization of bacterial nanocellulose (BNC) for large-scale applications is restricted by low productivity in static cultures and by the high cost of the medium. Fiber sludge, a waste stream from pulp and paper mills, was enzymatically hydrolyzed to sugar, which was used for the production of BNC by the submerged cultivation of Komagataeibacter xylinus. Compared with a synthetic glucose-based medium, the productivity of purified BNC from the fiber sludge hydrolysate using shake-flasks was enhanced from 0.11 to 0.17 g/(L × d), although the average viscometric degree of polymerization (DP v ) decreased from 6760 to 6050. The cultivation conditions used in stirred-tank reactors (STRs), including the stirring speed, the airflow, and the pH, were also investigated. Using STRs, the BNC productivity in fiber-sludge medium was increased to 0.32 g/(L × d) and the DP v was increased to 6650. BNC produced from the fiber sludge hydrolysate was used as an additive in papermaking based on the chemithermomechanical pulp (CTMP) of birch. The introduction of BNC resulted in a significant enhancement of the mechanical strength of the paper sheets. With 10% (w/w) BNC in the CTMP/BNC mixture, the tear resistance was enhanced by 140%. SEM images showed that the BNC cross-linked and covered the surface of the CTMP fibers, resulting in enhanced mechanical strength.

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Section: Resultssupporting

confidence: 92%

Bioconversion of Waste Fiber Sludge to Bacterial Nanocellulose and Use for Reinforcement of CTMP Paper Sheets

Chen

Alriksson³

et al. 2017

Polymers

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“…One prominent example is rotary disk reactors (RDRs). 33,34 These reactors increase productivity by up to 290% relative to ∼0.3 g L −1 h −1 under ordinary static reactor conditions; however, BC mechanical properties decrease with increased cultivation time in the RDR assembly. 35,36 The rotary disks also limit the geometries in which BC mats form.…”

Section: ■ Introductionmentioning

confidence: 99%

“…A bioreactor configuration that has been extensively studied uses low-speed rotational disks that introduce a constantly changing air–liquid interface for BC formation. One prominent example is rotary disk reactors (RDRs). , These reactors increase productivity by up to 290% relative to ∼0.3 g L –1 h –1 under ordinary static reactor conditions; however, BC mechanical properties decrease with increased cultivation time in the RDR assembly. , The rotary disks also limit the geometries in which BC mats form. Furthermore, they have not provided control over microstructural aspects of BC formation such as porosity, fibril diameter, density, and the regularity of these features.…”

Section: Introductionmentioning

confidence: 99%

Effect of Atomized Delivery of Nutrients on the Growth Characteristics and Microstructure Morphology of Bacterial Cellulose

Amason

Nowak²,

Samuel³

et al. 2019

Biomacromolecules

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This work demonstrates a general strategy for introducing remarkable changes in matrix organization and, consequently, functional properties of bacterial cellulose (BC). BC-producing cells were induced, using a well-defined atomized droplet nutrient delivery (ADND) system, to form pellicles with a regular layered morphology that persists throughout the mat depth. In contrast, the morphology of mats formed by conventional static medium nutrient delivery (SMND) is irregular with no distinguishable pattern. ADND also resulted in larger meso-scale average pore sizes but did not alter the fibril diameter (∼70 nm) and crystallinity index (92−95%). The specific modulus and specific tensile strength of ADND mats are higher than those of SMND mats. This is due to the regularity of dense layers that are present in ADND mats that are able to sustain tensile loads, when applied parallel to these layers. The density of BC films prepared by ADND is 1.63-fold lower than that of the SMND BC film. Consequently, the water contents (g/g) of ADND-and SMND-prepared BC mats are 263 ± 8.85 and 99.6 ± 2.04, respectively. A model that rationalizes differences in mat morphology resulting from these nutrient delivery methods based on nutrient and oxygen concentration gradients is proposed. This work raises questions as to the extent that ADND can be used to fine-tune the matrix morphology and how the resulting lower density mats will alter the diffusion of actives from the films to wound sites and increase the ability of cells to infiltrate the matrix during tissue engineering.

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“…This could be attributed to the facts that the glucose was transformed into other product than BC such as gluconic acid via direct oxidation during production process which finally led to significantly reduced in the pH of production medium. The pH value decreases during production because of the accumulation of byproducts like gluconic, acetic or lactic acids [26,27] Effect of different incubation temperatures on BC production.…”

Section: Impact Of Various Ph Values On Bc Productionmentioning

confidence: 99%

Production enhancement of bacterial cellulose nanofiber using local Komagataeibacter xylinus SB3.1 under static conditions

et al. 2021

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Bacterial cellulose (BC) is a nanostructured material mainly produced by genus Gluconacetobacter displays unique physicochemical and mechanical properties therefore it has many potential applications in biomedical, biosensor, food and other industries. However, application of bacterial cellulose faced one of the main big problems in industry, viz., low productivity. Herein, this work was undertaken with a view to enhance the BC production using Komagataeibacter xylinus SB3.1 under static condition through study the effective culture parameters that played a vital role in nanofiber production. The results revealed the successful production of BC nanofibers using K. xylinus SB3.1 under static conditions as obviously indicated from the SEM image. FTIR and XRD affirmed both the chemical structure and crystallographic nature of cellulose I of the produced nanofibers. In the improvement effort, maximum yield of BC reached 6.54 g/L under optimal conditions including the use of mannitol and yeast extract as the sole source of carbon and nitrogen during an 8 day incubation period at 30 •C with 8% inoculum size which reveals to the increase in the nanofiber production 3.3 folded times than others with unoptimized condition under the same stationary conditions of growth. This also opens up the window for utilizing a new locally isolated K. xylinus SB3 in industrial manufacturing with potential features.

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Process Parameters for Fermentation in a Rotary Disc Reactor for Optimum Microbial Cellulose Production using Response Surface Methodology

Cited by 25 publications

References 21 publications

Bioconversion of Waste Fiber Sludge to Bacterial Nanocellulose and Use for Reinforcement of CTMP Paper Sheets

Bioconversion of Waste Fiber Sludge to Bacterial Nanocellulose and Use for Reinforcement of CTMP Paper Sheets

Effect of Atomized Delivery of Nutrients on the Growth Characteristics and Microstructure Morphology of Bacterial Cellulose

Production enhancement of bacterial cellulose nanofiber using local Komagataeibacter xylinus SB3.1 under static conditions

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