Production enhancement of bacterial cellulose nanofiber using local Komagataeibacter xylinus SB3.1 under static conditions

Abstract

Bacterial cellulose (BC) is a nanostructured material mainly produced by genus Gluconacetobacter displays unique physicochemical and mechanical properties therefore it has many potential applications in biomedical, biosensor, food and other industries. However, application of bacterial cellulose faced one of the main big problems in industry, viz., low productivity. Herein, this work was undertaken with a view to enhance the BC production using Komagataeibacter xylinus SB3.1 under static condition through study the effective culture parameters that played a vital role in nanofiber production. The results revealed the successful production of BC nanofibers using K. xylinus SB3.1 under static conditions as obviously indicated from the SEM image. FTIR and XRD affirmed both the chemical structure and crystallographic nature of cellulose I of the produced nanofibers. In the improvement effort, maximum yield of BC reached 6.54 g/L under optimal conditions including the use of mannitol and yeast extract as the sole source of carbon and nitrogen during an 8 day incubation period at 30 •C with 8% inoculum size which reveals to the increase in the nanofiber production 3.3 folded times than others with unoptimized condition under the same stationary conditions of growth. This also opens up the window for utilizing a new locally isolated K. xylinus SB3 in industrial manufacturing with potential features.

Characterization of Bacterial Cellulose Produced by Komagataeibacter maltaceti P285 Isolated from Contaminated Honey Wine

“…The temperature is considered to play a critical role in the production of BC. Previous studies indicated that K. xylinus SB3.1 can produce BC at the temperature of 15–35 °C but not for 40 °C [ 52 ], while Acetobacter pasteurianus RSV-4 (MTCC 25117) shows the highest BC production at 30 °C [ 53 ]. On the other hand, K. maltaceti P285, K. pomaceti O277 and K. nataicola TISTR 975 were unable to produce BC at the temperature above 35 °C.…”

Bacterial cellulose: Biopolymer with novel medical applications

“…The optimal fermentation conditions for these organisms are a temperature of 25-30°C, pH in the range of 3-7 and the presence of sugars as the carbon source. 49 The carbon is later implemented in the pentose phosphate pathway or gluconeogenesis coupled Krebs cycle. BC is the final product of their metabolism and is synthesized with an efficiency of around 50%.…”

Production of bacterial cellulose from glycerol: the current state and perspectives

“…isolated from Kombucha tea (Trovatti et al 2011 ). Some recent studies on the use of mannitol in the production of BC have shown fairly good yield and productivity characteristics using the producers K. xylinus SB3.1 (Alemam et al 2021 ) and K. xylinus K2G30 (UMCC 2756) (Gullo et al 2019 ). Their levels, especially in the case of K2G30 (8.77 g/L; 0.041 g/L/h), indicate some potential for the industrial scale-up of BC production using vegetable waste feedstocks rich in mannitol and other sugar alcohols (Gullo et al 2019 ).…”