Effect of age on human peripheral blood stem cells.

Egusa, Yuria; Fujiwara, Yasuhiro; Syahruddin, Elisna; Isobe, Takeshi; Yamakido, Michio

doi:10.3892/or.5.2.397

Cited by 14 publications

(16 citation statements)

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“…Age analysis showed that high mobilizers were slightly younger (60.5 ± 2.5 vs. 66.8 ± 3.4, p < 0.05) and it is well known that PB-MNC-CD34+ counts decrease with age [6,23]. We therefore used a twofactor analysis of variance to determine whether age could be a confounding factor.…”

Section: Results J Circulating Pb-mnc-cd34+mentioning

confidence: 99%

“…Shintani et al [24] further reported a PB-MNC-CD34+ mobilization in patients with acute myocardial infarction. In vitro stimulation of human PB-MNC-CD34+ by VEGF leads to the release of cells that express macrophage, endothelial or neuronal markers and properties [6,17]. More recently, Taguchi et al [27] have shown that the administration of human CD34+ cells after stroke in a mouse model of middle cerebral artery occlusion, enhances neurogenesis: human CD34+ cells were shown to secrete some angiogenic factors including VEGF, HGF and IGF; these factors would contribute to the development of a pattern of newly formed vascularization which in turn would provide an optimal environment for neurogenesis.…”

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confidence: 99%

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Neurological and functional recovery in human stroke are associated with peripheral blood CD34+ cell mobilization

et al. 2007

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Section: Results J Circulating Pb-mnc-cd34+mentioning

confidence: 99%

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confidence: 99%

Neurological and functional recovery in human stroke are associated with peripheral blood CD34+ cell mobilization

et al. 2007

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“…Despite the important advances in characterization of HSCs progenitors, only in few reports the relationship between age and stem cells in humans has been examined (Hirota et al 1988;Nilsson-Ehle et al 1995;Egusa et al 1998;Bagnara et al 2000). Earlier studies described age-related numerical and functional alterations of progenitor cells in bone marrow (Hirota et al 1988;Nilsson-Ehle et al 1995).…”

Section: Introductionmentioning

confidence: 99%

Age- and gender-related alterations of the number and clonogenic capacity of circulating CD34+ progenitor cells

et al. 2005

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The aim of this study was to evaluate the peripheral representation and the clonogenic capacity of CD34(+) progenitor cells from 130 healthy subjects (80 females and 50 males) ranging in age from 16 to 100 years. We demonstrated that the absolute number of circulating CD34(+) cells progressively and significantly decreased with advancing age, with a 2-fold reduction in subjects aged more than 80 years. The number of granulocyte-macrophagic (CFU-GM), erytroid (BFU-E), and mixed (CFU-GEMM) colonies which developed from the number of CD34(+) purified cells per ml, progressively and significantly decreased with advancing age. The reduction of both CD34(+) cell number and clonogenic capacity during aging was statistically significant in males but not in females. When evaluated on a per cell bases, a significant age-related decrease in the number of CFU-GM colonies was observed in female but not in male subjects. Our study demonstrates the influence of gender on age-related alterations of the number and clonogenic capacity of CD34(+) cells in the peripheral blood. This evidence deserves particular consideration for the future planning of stem cell therapy in age-associated debilitating diseases.

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“…Further studies considered the influence of age on the amount of circulating endothelial progenitor cells (cEPC), a subpopulation of pPC, in the peripheral blood of healthy individuals and also showed no correlation, whereas Tao et al [28] found a dependence on age in men [29][30][31]. Two studies demonstrated a statistically significant reduction of circulating CD34+ cells in males of advancing age but not in females [32,33]. A comparison of the results of our study and results of the studies mentioned above, which analyzed CD34+ stem cells or the cEPC subpopulation, is problematic as our investigation focused primarily on pPC.…”

Section: Discussionmentioning

confidence: 99%

CD34+ and CD133+ Primitive Stem Cell Expression in Peripheral Blood: Considering Gender, Age, and Smoking

Reichelt¹,

Barz²,

Thude³

2009

Transfus Med Hemother

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Background: The number of primitive progenitor cells (pPC) in healthy individuals, in correlation to age, gender, and smoking status, has not yet been thoroughly elucidated. Material and Methods: The pPC from a collective of 168 healthy blood donors aged 18–61 years was investigated using flow cytometric analysis. In addition, the pPC of 20 subjects were studied once a month for half a year to determine the extent of physiological variation of pPC within a single individual. Results: We demonstrated a statistically significant difference (p = 0.005) in the numbers of pPC in men (836,100/l) versus women (583,850/l). No statistical difference was found between younger and older donors or between smokers and non-smokers, both overall and within a single gender. The extent of physiological variation in pPC was lower than 20% in 2 individuals, 18 individuals exhibited amplitudes greater than 20%. Conclusion: We conclude that the number of pPC in healthy individuals was primarily determined by gender as an operative factor. It seems that age and smoking status are of minor importance. Furthermore, our data demonstrate strong variability in the expression of pPC within a single individual. This may be influenced by varying physiological and environmental factors.

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Effect of age on human peripheral blood stem cells.

Cited by 14 publications

References 0 publications

Neurological and functional recovery in human stroke are associated with peripheral blood CD34+ cell mobilization

Neurological and functional recovery in human stroke are associated with peripheral blood CD34+ cell mobilization

Age- and gender-related alterations of the number and clonogenic capacity of circulating CD34+ progenitor cells

CD34+ and CD133+ Primitive Stem Cell Expression in Peripheral Blood: Considering Gender, Age, and Smoking

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