CD34+ and CD133+ Primitive Stem Cell Expression in Peripheral Blood: Considering Gender, Age, and Smoking

Abstract

Background: The number of primitive progenitor cells (pPC) in healthy individuals, in correlation to age, gender, and smoking status, has not yet been thoroughly elucidated. Material and Methods: The pPC from a collective of 168 healthy blood donors aged 18–61 years was investigated using flow cytometric analysis. In addition, the pPC of 20 subjects were studied once a month for half a year to determine the extent of physiological variation of pPC within a single individual. Results: We demonstrated a statistically significant difference (p = 0.005) in the numbers of pPC in men (836,100/l) versus women (583,850/l). No statistical difference was found between younger and older donors or between smokers and non-smokers, both overall and within a single gender. The extent of physiological variation in pPC was lower than 20% in 2 individuals, 18 individuals exhibited amplitudes greater than 20%. Conclusion: We conclude that the number of pPC in healthy individuals was primarily determined by gender as an operative factor. It seems that age and smoking status are of minor importance. Furthermore, our data demonstrate strong variability in the expression of pPC within a single individual. This may be influenced by varying physiological and environmental factors.

Clinically Oriented Research in Transfusion Medicine

“…Meanwhile an immunomagnetic purification technique for this antigen is available for clinical use. As shown by Reichelt et al [2], mobilization of these cells significantly differs only between sexes. Apparently in male donors more stem cells can be mobilized than in female donors.…”

“…Reichelt et al [2] describe the effects of age, sex, and smoker status on mobilization of CD34+/CD133+ stem cells. CD133 antigen was first described 10 years ago and is co-expressed on approximately 75% of CD34+ hematopoietic stem cells, especially on CD34 bright cells.…”

Stem‐cell mobilization of healthy sibling donors with pegfilgrastim—A prospective open‐label phase II trial (EudraCT no: 2005‐004971‐39)

“…Furthermore, we analyzed the yields of primitive stem cells expressing CD34+/CD133+ antigen 16 . In our collections the CD34+/CD133+ cells yielded with 4.45 × 10 6 /kg body weight of recipient (range 2.1–12.5).…”

Sitting decreases endothelial microparticles but not circulating angiogenic cells irrespective of lower leg exercises: a randomized cross‐over trial

“…However, it was recently demonstrated that women exhibit unique vascular responses to sitting (Credeur et al., 2019; Vranish et al., 2017) and that exercise‐induced endothelial MP release differs across sex (Shill et al., 2018) and should be considered in future trials. Oral contraceptive use was not reported, as limited data suggest that there are no effects of contraceptives on baseline levels of CAC (Reichelt, Barz, & Thude, 2009; Shill et al., 2016). To examine the influence of the hormonal fluctuations on CAC or MPs (Shill et al., 2018), β‐oestradiol and progesterone were assessed and remained constant across both visits, and appear to be non‐factors.…”