Effect of Addition of Water-Soluble Polysaccharides on Bacterial Cellulose Production in a 50-L Airlift Reactor

Chao, Yaping; Mitarai, Makoto; Sugano, Yasushi; Shoda, Makoto

doi:10.1021/bp010046b

Cited by 55 publications

(25 citation statements)

References 19 publications

(26 reference statements)

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“…Although other polymeric additives such as xanthan, agar, acetan were investigated to hinder formation of large clumps of BC and enhance BC production in either jar fermentor or airlift reactor, there are remarkable diVerences in BC enhancing extent according to diVerent strains, additives and additives concentration [1,3,9]. Bae et al [1] reported that the maximum BC production of A.xylinum BPR2001 at 0.4% (w/v) agar was 12.8 g/l compared with xylinum NUST4.1 in the medium containing NaAlg in the shake Xask.…”

Section: Discussionmentioning

confidence: 99%

“…Recently there are a few reports stating that addition of water-soluble polymers such as xanthan, agar, polyacrylamide-co-acylic acid (PCA), and acetan can increase relative viscosity of the broth to reduce shear stress, hinder coagulation of BC during the cultivation to form uniform smaller pellets, which are advantageous to transfer nutrients and oxygen into bacterial cells located inside and on the surface of the cellulose matrix, and further promote BC productivity into the medium, will assist in enhancing BC productivity [1,3,9,10]. On the other hand, various watersoluble polymers added into the medium are known to interfere with the aggregation of microWbrils into a normal ribbon assembly, aVect the crystallization of cellulose I and I and introduce the characteristic properties of these additives to attain BC composite materials [6].…”

Section: Introductionmentioning

confidence: 99%

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Effect of addition of sodium alginate on bacterial cellulose production by Acetobacter xylinum

Zhou

Sun

et al. 2007

J Ind Microbiol Biotechnol

142

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Bacterial cellulose (BC) production by Acetobacter xylinum NUST4.1 was carried out in the shake flask and in a stirred-tank reactor by means of adding sodium alginate (NaAlg) into the medium. When 0.04% (w/v) NaAlg was added in the shake flask, BC production reached 6.0 g/l and the terminal yield of the cellulose was 27% of the total sugar initially added, compared with 3.7 g/l and 24% in the control, respectively. The variation between replicates in all determinations was less than 5%. During the cultivation in the stirred-tank reactor, the addition of NaAlg changed the morphology of cellulose from the irregular clumps and fibrous masses entangled in the internals to discrete masses dispersing into the broth, which indicates that NaAlg hinders formation of large clumps of BC, and enhances cellulose yield. Because the structure of cellulose is changed depending on the culture condition such as additives, structural characteristics of BC produced in the NaAlg-free and NaAlg medium are compared using scanning electron microscopy (SEM), fourier transform infrared spectroscopy (FT-IR), X-ray diffraction (XRD). SEM photographs show some differences in reticulated structures and ribbon width and FT-IR spectra indicate that there is the hydrogen bonding interaction between BC and NaAlg, then X-ray diffraction (XRD) analysis reveals that BC produced with NaAlg-added has a lower crystallinity and a smaller crystalline size. The results show that enhanced yields and modification of cellulose structure occur in the presence of NaAlg.

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Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Effect of addition of sodium alginate on bacterial cellulose production by Acetobacter xylinum

Zhou

Sun

et al. 2007

J Ind Microbiol Biotechnol

142

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“…The production of biodegradable thermoplastics from bacterial polyhydroxyalkanoates synthesized by Ralstonia eutropha in a membrane ALR (446) and a DT-ALR (447) has been examined. Obtaining of bacterial cellulose from cultures of Acetobacter xylinum in a DT-ALR has also been documented (448,449).…”

Section: Biopolymersmentioning

confidence: 99%

Bioreactors: Airlift Reactors

Merchuk

Camacho

2010

Encyclopedia of Industrial Biotechnology

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ALRs are popular in modern bioprocess research and development and, in many cases, they have found industrial uses. The distinctive characteristics of ALRs are conferred by the fluid dynamics of the liquid‐gas or liquid gas‐solid mixtures: holdup, liquid velocity, and mixing in the riser, gas separator, and downcomer. Only a correct understanding of the interconnection of these regions can make possible the scale‐up of a laboratory device, to pilot or industrial size. Several correlations are available in the literature for the prediction of the fluid dynamic characteristics of the reactor and of the mass and heat transfer coefficients, and a selection is presented in this review. Significant progress has been made in the application of modern computational tools to the simulation and design of ALRs. In parallel, innovative and sophisticated methods have been applied to obtain a deeper insight into the mechanisms of fluid motion and the flow patterns in the reactor. However, no single research group has managed to cover all the variables over a wide range. The engineer confronting scale‐up or de novo design of an ALR must analyze the validity of the correlations and computation methods used for the calculations.

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“…The concentration of BC was doubled and the production rate was 0.116 g L −1 h −1 , which is twice that of an air supplied culture and comparable with that in a mechanically agitated stirred-tank fermentor. Chao et al 40 using the 50 L internal-loop airlift reactor discovered that when 0.1% (w/w) agar (water-soluble polysaccharide) was added, BC production reached 8.7 g L −1 compared with 6.3 g L −1 in the control medium using Acetobacter xylinum subsp. sucrofermentans (BPR2001).…”

Section: Effect Of the Type Of Reactor On Productionmentioning

confidence: 99%

Improvements in the production of bacterial synthesized biocellulose nanofibres using different culture methods

Sani

Dahman

2009

J of Chemical Tech & Biotech

111

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This review summarizes previous work that was done to improve the production of bacterial cellulose nanofibres. Production of biocellulose nanofibres is a subject of interest owing to the wide range of unique properties that makes this product an attractive material for many applications. Bacterial cellulose is a natural nanomaterial that has a native dimension of less than 50 nm in diameter. It is produced in the form of nanofibres, yielding a very pure cellulose product with unique physical properties that distinguish it from plant-derived cellulose. Its high surface-to-volume ratio combined with its unique properties such as poly-functionality, hydrophilicity and biocompatibility makes it a potential material for applications in the biomedical field. The purpose of this review is to summarize the methods that might help in delivering microbial cellulose to the market at a competitive cost. Different feedstocks in addition to different bioreactor systems that have been previously used are reviewed. The main challenge that exists is the low yield of the cellulosic nanofibres, which can be produced in static and agitated cultures. The static culture method has been used for many years. However, the production cost of this nanomaterial in bioreactor systems is less expensive than the static culture method. Biosynthesis in bioreactors will also be less labour intensive when scaled up. This would improve developing intermediate fermentation scale-up so that the conversion to an efficient large-scale fermentation technology will be an easy task.

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Effect of Addition of Water-Soluble Polysaccharides on Bacterial Cellulose Production in a 50-L Airlift Reactor

Cited by 55 publications

References 19 publications

Effect of addition of sodium alginate on bacterial cellulose production by Acetobacter xylinum

Effect of addition of sodium alginate on bacterial cellulose production by Acetobacter xylinum

Bioreactors: Airlift Reactors

Improvements in the production of bacterial synthesized biocellulose nanofibres using different culture methods

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