Effect of actinomycin D on mice infected with the lactate dehydrogenase virus

Crispens, Charles G.

doi:10.1007/bf01897439

Cited by 7 publications

(3 citation statements)

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“…Interferon has been demonstrated in the sera of LDV-infected mice in the first few days after infection (3,7,10), and it is quite likely that interferon plays some role in the reduction of viremia in this infection. Limited support for this suggestion comes from experiments with mice, in which administration of actinomycin, which suppresses interferon formation, resulted in increased serum titers of LDV during the first week of infection (5). Additional evidence is derived from the X-irradiation experiments reported previously (8).…”

Section: Discussionmentioning

confidence: 99%

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Effect of an Immunosuppressive Agent, Cyclophosphamide, on Chronic Lactic Dehydrogenase Virus Viremia of Mice

1971

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The immunosuppressive drug cyclophosphamide was used to study the effect of immunosuppression on the degree of chronic lactic dehydrogenase virus (LDV) viremia in mice. Weekly injections of cyclophosphamide caused an increase of LDV viremia of ca. 2.5 logio 50% infectious doses per ml as compared to the viremia in the nontreated mice. This increase occurred at about the same time that the formation of antibody with a neutralization index of 2 to 3 logio per ml could be demonstrated in the serum of control mice, whereas both neutralizing antibody and sensitized (antibody-bound but infectious) virus were absent in the immunosuppressed mice. In one experiment, antibody did not reappear 28 weeks after a 24-week course of cyclophosphamide treatment. In another experiment in which the drug was given for 12 weeks, antibody appeared again in the treated mice 14 weeks after cessation of drug administration and was accompanied by a decrease in viremia. The normal decrease of early viremia during the first 2 weeks of infection in immunosuppressed mice suggests that the immune response is not the cause of this initial decrease in LDV viremia but that interferon and perhaps other nonimmune factors may be involved here. The increased viremia in the immunosuppressed mice during the later stages of infection indicates that the immune response plays an important role in partial control of chronic viremia. The quantitative correlation between the decrease in neutralizing antibody and the increase in viremia in immunosuppressed mice suggests that antibody rather than cellular immunity may be the more important immune factor in the control of chronic viremia.Mice infected with lactic dehydrogenase virus (LDV; reference 17) present a useful model for a study of the role of immune and nonimmune factors in controlling viremia in a chronic viral infection. The viremia in LDV-infected mice lasts for the lifetime of the mice without any overt pathology (14). Serum interferon can be detected during the first few days of infection (3, 7, 10), and neutralizing antibody can be detected after a few weeks (15, 19); however, the relative roles of interferon and antibody, as well as that of cellular immunity, in determining the level of viremia in this infection remain unclear.In an attempt to clarify the importance of immune processes in this chronic infection, we have studied the effect of a potent immunosuppressive agent, cyclophosphamide, on viremia and on the formation of neutralizing antibody and sensitized virus in LDV-infected mice. MATERIALS AND METHODSAnimals. Random bred Swiss albino (15 to 20 g, female) mice were supplied by the animal production section of the National Institutes of Health.Virus. The strain of LDV used was isolated from a naturally infected Ehrlich ascites carcinoma carried in CDF, mice (7). Stock LDV was obtained from LDVfree Ehrlich ascites tumor-bearing mice, which were infected intraperitoneally with 107°50.% infectious doses (ID50) per mouse 6 to 8 days after tumor implantation. Twenty-four hours after infection...

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Section: Discussionmentioning

confidence: 99%

“…30: 241, 1971; 16). It has been reported that neonatal thymectomy will also result in a higher chronic viremia in LDV-infected mice (6), and it was suggested that this was because of decreased neutralizing antibody formation. Since antibody was not measured, it is not possible to know if this suggestion is correct.…”

Section: Discussionmentioning

confidence: 99%

Effect of an Immunosuppressive Agent, Cyclophosphamide, on Chronic Lactic Dehydrogenase Virus Viremia of Mice

1971

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“…LDH Virus The source of the LDH virus has been described elsewhere (9). LDH Virus The source of the LDH virus has been described elsewhere (9).…”

Section: Animalsmentioning

confidence: 99%

Studies on the response of SJL/J mice to infection with the lactate dehydrogenase virus

Crispens

1971

Archiv f Virusforschung

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Infection of SJL/J mice with the lactate dehydrogenase virus resulted in a greater increase in the plasma activities of lactate dehydrogenase, malate dehydrogenase, and isocitrate dehydrogenase than expected from findings with other inbred strains. Evidence is presented to suggest that the differences are due to an exaggerated reduction in the clearance of these enzymes. It is postulated that the data signify a deficiency in the function of the reticuloendothelial system of SJL/J mice. 178 C.G. C~ise~NS, JR.: colony that has since been maintained, by brother and sister rantings, in our Animal Services Center. Most of the experiments utilized male as well as female animals, 8 to 12 weeks old. However, except for the initial studies on plasma LDH levels, only data obtained from female mice will be reported. Strain BALB/c mice of the same ages and of both sexes were used for controls. These were supplied, as needed, through the courtesy of Dr. CEARLnS P. DAGG. None of the animals had reticulum cell neoplasms at autopsy, which was never later than 16 weeks of age.

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Lactic Dehydrogenase Virus

Rowson

Mahy

1975

Lactic Dehydrogenase Virus

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Effect of actinomycin D on mice infected with the lactate dehydrogenase virus

Cited by 7 publications

References 0 publications

Effect of an Immunosuppressive Agent, Cyclophosphamide, on Chronic Lactic Dehydrogenase Virus Viremia of Mice

Effect of an Immunosuppressive Agent, Cyclophosphamide, on Chronic Lactic Dehydrogenase Virus Viremia of Mice

Studies on the response of SJL/J mice to infection with the lactate dehydrogenase virus

Lactic Dehydrogenase Virus

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