Studies on the response of SJL/J mice to infection with the lactate dehydrogenase virus

Abstract

Infection of SJL/J mice with the lactate dehydrogenase virus resulted in a greater increase in the plasma activities of lactate dehydrogenase, malate dehydrogenase, and isocitrate dehydrogenase than expected from findings with other inbred strains. Evidence is presented to suggest that the differences are due to an exaggerated reduction in the clearance of these enzymes. It is postulated that the data signify a deficiency in the function of the reticuloendothelial system of SJL/J mice. 178 C.G. C~ise~NS, JR.: colony that has since been maintained, by brother and sister rantings, in our Animal Services Center. Most of the experiments utilized male as well as female animals, 8 to 12 weeks old. However, except for the initial studies on plasma LDH levels, only data obtained from female mice will be reported. Strain BALB/c mice of the same ages and of both sexes were used for controls. These were supplied, as needed, through the courtesy of Dr. CEARLnS P. DAGG. None of the animals had reticulum cell neoplasms at autopsy, which was never later than 16 weeks of age.

Identification of lactate dehydrogenase-elevating virus as the etiological agent of genetically restricted, age-dependent polioencephalomyelitis of mice

“…Immunosuppressed old SJL mice were found to be resistant to the induction of ADPE (data not shown). LDV-infected SJL mice have been previously reported to display a twofold greater increase in plasma lactate dehydrogenase levels than do other strains of mice tested (5). This trait is inherited as a recessive Mendelian allele (6).…”

Genetic basis for Ia positivity and susceptibility to lactic dehydrogenase virus in macrophages of SJL/J mice

“…Crispens reported that the higher elevation of serum LDH and MDH enzymes in LDV infected SJL/J mice compared with BALB/c mice was a character inherited as a single autosomal recessive trait (1,2). We have shown in this paper that in peritoneal macrophages of SJL/J mice, the per cent that are Ia positive is 3-5 times higher than in the other mouse strains tested.…”

“…Crispens reported that SJL/J mice were highly susceptible to LDV infection in so far as the elevation of serum lactic dehydrogenase (LDH) and malic dehydrogenase (MDH) activity after LDV infection was 2-3 times higher than in BALB/c mice (1). He showed that this character is regulated by a single autosomal recessive gene (2).…”

Genetic control of the response of SJL/J mice to LDH virus infection

“…The method for collection and storage of plasma has been described elsewhere (2). LDH and MDI-I were assayed speetrophotometrieally by the methods of HENRY et al (4) and MEHLE~ et al (5), respectively.…”