Efektivitas ekstrak umbi sarang semut (myrmecodia pendens merr.& perry) sebagai penurun kadar glukosa darah tikus sprague dawley yang diabetes mellitus

Abstract

Background : Diabetes Mellitus (DM) is a disease associated with pancreatic β cell damage and causes hyperglycemia Antioxidants were effective in reducing the state of hyperglycemia that was triggered by free radicals. Sarang semut contains flavonoids and tannins that act as antioxidants.Objective : The purpose to analyze the effectiveness of sarang semut stem extract as lowering blood glucose on diabetic white male Sprague Dawley rats. Method : True experimental with 30 rats which divided into five groups : negative control (DM+ water), positive control (DM+glibenklamid) and 3 treatment groups of DM + water extract of sarang semut stem powder 90 mg/200gBW, 180 mg/200g BW and 360/200g BW respectively. DM was induced by 65mg/kgBW Streptozotocin and 230 mg/kgBW Nicotinamide intraperitonealy. Extract was given by sonde. The data was analyzed by Kruskall-Wallis and Mann-Whitney. Results : IC50 value of sarang semut stem extract were 6,56-10,07 mg/ml in powder and 9,12-15,62 mg/ml whole. The average rats weight alteration on groups of negative control, positive control, and 3 treatment groups of DM + water extract of sarang semut stem powder 90 mg/200gBW, 180 mg/200g BW and 360/200g BW before and after treatment were -7,60± -0,40; 25,80±0,51; 16,40±0,44; 17,20±1,28 dan 19,20±0,06 gram respectively (ρ=0,001). The average fasting blood glucose level on groups of negative control, positive control, and 3 treatment groups of DM + water extract of sarang semut stem powder 90 mg/200gBW, 180 mg/200g BW and 360/200g BW before and after treatment were 3,33±2,42; -122,44±-0,51; -79,42±-11,38; -94,08±-11,97 and -134,66±-2,59 mg/dL respectively (ρ=0,001). Conclusion : The IC50­ value in sarang semut stem powder was higher than a whole form. Dose of 360 mg/200g BW lowered blood glucose better than 90 and 180 /200g BW dosage.

Distribution and FTIR-based fingerprint of secondary metabolites in different organs of ant-plant (Myrmecodia tuberosa)

“…The ethanol extract of domatium of this species was reported to reduce tumor growth and induce apoptosis of oral carcinoma cells (Yuletnawati et al 2016) and had a cytotoxic effect on MCF-7, HT-29 and HELA cells (Rasemi et al 2014). In addition, the domatium was potentially antidiabetic (Raya et al 2016). Those medicinal effects might be attributed to the flavonoid flavonols contained in the domatium.…”

Suplementasi Soya Yogurt Untuk Perbaikan Kelimpahan Mikroba Sekum (Bakteri Asam Laktat Dan Escherichia coli) Tikus Diabetes Soya Yogurt

“…Hasil pengkondisian tikus diabetes ini mendukung beberapa penelitian lain terkait efektifitas dari induksi NA dan STZ pada model hewan uji diabetes melitus. Induksi NA 230 mg/kg BB dan STZ 65 mg/kg BB dosis tunggal juga dilaporkan efektif dalam menginduksi tikus diabetes dengan menimbulkan hiperglikemia dengan kadar gula darah > 250 mg/dL (Ghasemi et al, 2014;Raya et al, 2016) (Palupi et al, 2019). Signifikansi peningkatan kadar gula darah akibat induksi NA dan STZ menurut Ghasemi et al (2014) sangat bervariasi.…”