Efek Imunomodulator Ekstrak Etanol Spons Melophlus sarasinorum Terhadap Aktivitas Fagositosis Sel Makrofag Pada Mencit Jantan Balb/C

Abstract

Immunomodulator is an ingredient or drug that can modulate immune system functions and activities. This study was conducted to determine the effect of ethanol extract of Melophlus sarasinorum sponge on macrophage phagocytosis activity. Twenty four male mice balb/c were divided into six groups. The first group received 100 mg/kg of ethanol extract of Melophlus Sarasinorum sponge, the second group received 200 mg/kg of ethanol extract of Melophlus sarasinorum sponge, the third group received 300 mg/kg of ethanol extract of Melophlus Sarasinorum sponge and the fourth group received 400 mg/kg of ethanol extract of Melophlus Sarasinorum sponge. The positive control group received Phyllanthus niruri linn extract (Stimuno®) 0,13 mg/g and the negative control group received NaCMC 0,5%. The extract was orally administered from first day to seventh day. On the eighth day, each of the mice was injected Staphylococcus aureus bacteria (SA) 0.5 mL intraperitoneally. Macrophage cell activity is calculated from smears of peritoneal fluid of mice. Increased doses of ethanol extract of Melophlus sarasinorum sponge increase the amount of macrophage phagocytosis activity that are 25,25% (negative control), 61,5% (positive control), 55,75% (100 mg/kg), 60,75% (200 mg/kg), 62,25% (300 mg/kg) dan 66,25% (400 mg/kg). The results showed that the ethanol extract of Melophlus sarasinorum sponge has the potential as immunomodulator at a doses of 300 mg/kgBB and 400 mg/kg with no significantly different effectiveness with positive control in increasing macrophage cell phagocytosis activity based on the result of post-hoc statistical test of Tukey (sig.> 0,05).

Immunomodulatory activity and phytochemical analysis of Hibiscus sabdariffa L. flower fractions

“…Therefore, macrophage extraction was carried out approximately for 1 hour after bacterial induction. In this manner, to which extent the macrophages were able to deal with bacterial invasion could be identified (Wahyuni et al, 2019).…”

“…Cytokines that are secreted by macrophages include interleukin (IL)-1, IL-6, IL-8, IL-12, IL-15, and tumor necrosis factor (TNF-α). Under a normal condition, this process runs slowly, so the active macrophages that undergo phagocytosis resulting are small in quantity (Wahyuni et al, 2019).…”

PENINGKATAN SITOKIN INTERFERON GAMA TIKUS WISTAR SETELAH DIBERIKAN EKSTRAK ETANOL BUAH Etlingera rubroloba A.D. Poulsen SEBAGAI IMUNOSTIMULATOR

“…Penelitian oleh Ilyas et al, (2022) dimana ekstrak etanol buah E. rubroloba mengandung senyawa metabolit sekunder golongan flavonoid, alkaloid, saponin, tannin dan terpenoid. Senyawa flavonoid memiliki efek imunostimulan dikarenakan flavonoid jenis flavonol glikosida yang bersifat mitogen yang diduga melalui produksi sitokin IL-2, IL-4 dan IL-1 sehingga dapat memacu proliferasi dari sel limfosit T dan limfosit B (Fristiohady et al, 2020;Wahyuni et al, 2019). Proliferasi sel limfosit T akan meningkatkan aktivitas dari limfosit T sitotoksik (CD8) dan limfosit T helper (CD4) (Febrianty dan Sasmito, 2015;Dewi at al., 2013).…”

The role of indigenous lactic acid Bacteria Genus Lactobacillus in the fermentation process of Durian (Durio zibethinus) for Tempoyak production

“…Untuk mekanisme bakterisidal dapat berupa peningkatan fungsi perlindungan, efek imunomodulator serta antimikroba. Wahyuni et al (2019), imunomodulator merupakan senyawa yang dapat mengembalikan ketidakseimbangan sistem imun dengan cara mengembalikan fungsi sistem imun yang terganggu (imunorestorasi), meningkatkan fungsi sistem imun (imunostimulan) dan menekan respon imun (imunosupresi).…”