Efeitos da ocratoxina no desempenho do camarão-branco-do-pacífico ( Litopenaeus vannamei, Bonne)

Albuquerque, Waleska Ferreira de; Calvet, Rodrigo Maciel; Rocha, Clarice; Martins, Pedro Carlos Cunha; Nóbrega, Maria Marlucia Gomes Pereira; Costa, Amilton Paulo Raposo; Muratori, Maria Christina Sanches

doi:10.1590/1678-4162-8525

Cited by 3 publications

(3 citation statements)

References 18 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Hepatotoxic and nephrotoxic effects of AFB 1 on animals (Santacroce et al 2008;Williams et al 2004;2003;Creppy 2002) can increase tambaqui fingerlings susceptibility to acquiring certain pathologies as other aquatic animals assayed (Calvet 2012;Albuquerque 2013).…”

Section: Discussionmentioning

confidence: 99%

See 1 more Smart Citation

Effects of aflatoxin B1 on performance and health of tambaqui fingerlings (Colossoma macropomum)

et al. 2019

View full text Add to dashboard Cite

The detection of mycotoxins in feeds and their ingredients in aquaculture gained prominence due to losses caused in production and animal health, mainly the occurrence of aflatoxin (AFB 1 ). The aim of this study was to evaluate the effects of AFB 1 on the performance of tambaqui fingerlings (Colossoma macropomum). Four hundred tambaqui were used. Four different treatments were evaluated: treatment T1, considered as the control treatment (CT) with 3.84 lg kg -1 ; treatment T2, treatment T3 and treatment T4 with 500, 1000 and 2000 lg kg -1 of AFB 1 , respectively. The AFB 1 of the samples (muscle, liver and kidney) was detected by high-pressure liquid chromatography. Four fingerlings from each treatment for histological analysis were examined. Moreover, the performance parameters (weight gain, feed conversion and feed intake) were studied. The levels of toxins used in T2, T3 and T4 represent a reduction in the growth of 14%, 35% and 45%, respectively. The T3 and T4 showed the lowest weight gain (78%) and the worst feed conversion. Aflatoxin B 1 in muscle (3.28 lg kg -1 ) and kidneys (8.8 lg kg -1 ) in the T3, as well as liver (4.4 lg kg -1 ) and kidney (4.08 lg kg -1 ) in T4, was detected. Histopathological changes in liver and kidney tissues of fingerlings were more pronounced in T3 and T4. Fingerlings that consume feed contaminated with AFB 1 in concentrations higher than 500 lg kg -1 present decreases in growth, reduction in weight gain and feed intake with increased feed conversion. The consumption of feed contaminated with 1000 and 2000 lg kg -1 of AFB 1 caused severe deterioration in the hepatic and renal tissues.

show abstract

Section: Discussionmentioning

confidence: 99%

“…The in vivo assay was performed in 16 polyethylene fish water tanks (250 L) using a continuous water flow system. The effluent generated in the experiment was decontaminated before being thrown into the environment using sodium hypochlorite 2-2.5% for 30 min and acetone for another 30 min (Calvet 2012;Albuquerque 2013).…”

Section: Animals and Installationsmentioning

confidence: 99%

Effects of aflatoxin B1 on performance and health of tambaqui fingerlings (Colossoma macropomum)

et al. 2019

View full text Add to dashboard Cite

show abstract

“…The growth of muscle in teleost fish is not predetermined at birth but rather follows a lifelong pattern characterized by myofiber hyperplasia and myofiber hypertrophy [ 45 ], which are dependent on the processes of myoblast proliferation, differentiation, and fusion [ 46 ]. It has been reported that OTA could contaminate over 80% of aquafeed [ 47 ], which not only brings huge economic losses to the aquaculture industry but also jeopardizes human health through food chain accumulation [ 48 ]. In this study, grass carp ( Ctenopharyngodon idella ), a significant economic species of fish, was utilized as a model.…”

Section: Introductionmentioning

confidence: 99%

Unveiling the emerging role of curcumin to alleviate ochratoxin A-induced muscle toxicity in grass carp (Ctenopharyngodon idella): in vitro and in vivo studies

Zhao,

Feng,

Jiang

et al. 2024

J Animal Sci Biotechnol

View full text Add to dashboard Cite

Background Ochratoxin A (OTA), a globally abundant and extremely hazardous pollutant, is a significant source of contamination in aquafeeds and is responsible for severe food pollution. The developmental toxicity of OTA and the potential relieving strategy of natural products remain unclear. This study screened the substance curcumin (Cur), which had the best effect in alleviating OTA inhibition of myoblast proliferation, from 96 natural products and investigated its effect and mechanism in reducing OTA myotoxicity in vivo and in vitro. Methods A total of 720 healthy juvenile grass carp, with an initial average body weight of 11.06 ± 0.05 g, were randomly assigned into 4 groups: the control group (without OTA and Cur), 1.2 mg/kg OTA group, 400 mg/kg Cur group, and 1.2 mg/kg OTA + 400 mg/kg Cur group. Each treatment consisted of 3 replicates (180 fish) for 60 d. Results Firstly, we cultured, purified, and identified myoblasts using the tissue block culture method. Through preliminary screening and re-screening of 96 substances, we examined cell proliferation-related indicators such as cell viability and ultimately found that Cur had the best effect. Secondly, Cur could alleviate OTA-inhibited myoblast differentiation and myofibrillar development-related proteins (MyoG and MYHC) in vivo and in vitro and improve the growth performance of grass carp. Then, Cur could also promote the expression of OTA-inhibited protein synthesis-related proteins (S6K1 and TOR), which was related to the activation of the AKT/TOR signaling pathway. Finally, Cur could downregulate the expression of OTA-enhanced protein degradation-related genes (murf1, foxo3a, and ub), which was related to the inhibition of the FoxO3a signaling pathway. Conclusions In summary, our data demonstrated the effectiveness of Cur in alleviating OTA myotoxicity in vivo and in vitro. This study confirms the rapidity, feasibility, and effectiveness of establishing a natural product screening method targeting myoblasts to alleviate fungal toxin toxicity. Graphical Abstract

show abstract

New perspective on mechanism in muscle toxicity of ochratoxin A: Model of juvenile grass carp (Ctenopharyngodon idella)

Zhao,

Liu,

Feng

et al. 2023

Aquatic Toxicology

View full text Add to dashboard Cite

Efeitos da ocratoxina no desempenho do camarão-branco-do-pacífico ( Litopenaeus vannamei, Bonne)

Cited by 3 publications

References 18 publications

Effects of aflatoxin B1 on performance and health of tambaqui fingerlings (Colossoma macropomum)

Effects of aflatoxin B1 on performance and health of tambaqui fingerlings (Colossoma macropomum)

Unveiling the emerging role of curcumin to alleviate ochratoxin A-induced muscle toxicity in grass carp (Ctenopharyngodon idella): in vitro and in vivo studies

New perspective on mechanism in muscle toxicity of ochratoxin A: Model of juvenile grass carp (Ctenopharyngodon idella)

Contact Info

Product

Resources

About