Efeito dos reguladores de crescimento dicamba e picloram na embriogênese somática em Eucalyptus grandis

Titon, Miranda; Xavier, Aloísio; Otoni, Wagner Campos; Motoike, Sérgio Yoshimitsu

doi:10.1590/s0100-67622007000300007

Cited by 11 publications

(17 citation statements)

References 22 publications

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“…According to Titon, Xavier, Otoni, and Motoike (2007), picloram is used for the induction of calli or the maintenance of cultures in suspensions, as well as for inducing the formation of embryogenic calli. Most studies have indicated that calli are achieved primarily through the use of 2,4-D (Bajpai, Kalim, Chandra, & Kamle, 2016;Fehér, 2015;Rocha et al, 2016;Winkelmann et al, 2015), including in the induction of calogenesis in Campomanesia rufa leaf explants (Sant'Ana, Paiva, Reis, Silva, & Silva, 2018), which was not found in the present study.…”

Section: Discussionmentioning

confidence: 99%

Embryogenic potential of the callus of gabirobeira, Campomanesia adamantium (Cambess) O. Berg

Rossato

Schumacher

Netto

et al. 2019

Acta Sci. Biol. Sci.

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Campomanesia adamantium is a native plant species of Brazilian Cerrado with diverse economic potential and great medicinal importance. Its sexual propagation is impaired by the recalcitrance of its seeds, which prevents effective and profitable propagation. With the purpose of establishing commercial crops and minimizing the extractive use of vegetal resources, the aim of the present study was to induce embryogenic calli in nodal segments of gabirobeira, and to determine and characterize their embryogenic phase through the establishment of a growth curve based on cellular characteristics. Calli were induced using nodal segments inoculated in WPM culture medium without the addition of hormones (control) and with different concentrations of 2,4-D, IAA, IBA, NAA or picloram. Cytochemical and SEM analyses revealed cellular characteristics of the formation of meristematic centers that indicated 4.14 μM of picloram to be the best treatment for induction of embryogenic calli, and demonstrating their embryogenic potential. The treatment was used to establish a callus growth curve, from which it was inferred that calli should be transferred to new culture media on the 28th day to maintain cell viability

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Section: Discussionmentioning

confidence: 99%

Embryogenic potential of the callus of gabirobeira, Campomanesia adamantium (Cambess) O. Berg

Rossato

Schumacher

Netto

et al. 2019

Acta Sci. Biol. Sci.

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“…Somatic embryo development was achieved in a single step in the presence of dicamba, which has been reported to enhance the induction of SE in other tropical timber species, such as Eucalyptus grandis (Titon et al 2007) and E. globulus (Pinto et al 2002); however, dicamba is the least used PGR in SE protocols (Jiménez 2005).…”

Section: Development Of Somatic Embryosmentioning

confidence: 99%

Induction of somatic embryogenesis and plant regeneration in the tropical timber tree Spanish red cedar [Cedrela odorata L. (Meliaceae)]

Peña-Ramírez

García-Sheseña

Hernández-Espinoza

et al. 2010

Plant Cell Tiss Organ Cult

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Spanish red cedar (Cedrela odorata L.) is a tropical timber tree native to the Americas from southern Mexico to northern Argentina. Commercial plantations are scarce and, consequently, natural populations are overexploited. Traditional propagation practices for the establishment of large-scale plantations have had limited success in this species due to the relative scarcity of seeds, its broad genetic diversity and the lack of domesticated varieties. In vitro clonal propagation provides an effective method to overcome this situation and increase the yield of commercial plantations through the rapid multiplication of elite materials. Somatic embryogenesis (SE) is one of the most promising strategies for tree propagation due to the possibility of producing artificial seeds, the ability to store and rapidly mobilize germplasm and the opportunity for genetic manipulation. We report here the induction of indirect SE in C. odorata from calli derived from immature zygotic embryos after 12 weeks of culture. Macroscopic, histological, and scanning electron microscopic analyses of the calli revealed the presence of embryogenic cell clusters that formed cotyledonary embryos with clear bipolar structures and no vascular connections with the mother tissue. Different media preparations containing combinations of diverse auxins and cytokinins are known to have different effects on the type and frequency of embryogenic structures. Embryo conversion was achieved using an MS-based medium [Murashige T, Skoog F (1962) Physiol Plant 15:473-497, 1962 supplemented with abscisic acid, and transfer to soil was successful at a rate of 75%. The method described here provides a basis for optimizing the clonal propagation and genetic manipulation of this valuable species.

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“…Organogenesis in Eucalyptus is widely reported in the literature as successful 1,6,18 , while studies on somatic embryogenesis are few. Somatic embryogenesis has drawn special interest from research institutions and Brazilian forestry companies, which have advanced plant breeding and cloning programs 30 since the technique allows propagation on a larger scale than organogenesis and, moreover, minimizes the occurrence of chimeras generated by the organogenic route 33,8 . Somatic embryogenesis is the process by which somatic cells change their path of development, becoming totipotent, and one cell alone is able to originate a complete plant with the formation of somatic embryos 9 , by either a direct or indirct route.…”

Section: Introductionmentioning

confidence: 99%

“…Direct somatic embryogenesis consists of formation of somatic embryos directly from the explants, without the formation of the intermediate stage of the callus, and the indirect occurs through callus formation. This is more advantageous than the direct route by the fact of obtaining a larger number of plants 20 30 . Nevertheless, somatic embryogenesis for Eucalyptus still faces some difficulties, such as the low frequency of callus and somatic embryo induction and lack of efficiency in conversion and regeneration of plants 23,25 .…”

Section: Introductionmentioning

confidence: 99%

Morpho and Cytological Differentiation of Calli of Eucalyptus grandis x Eucalyptus urophylla During Somatic Embryogenesis

Batista

Mendonça

Pádua

et al. 2018

Braz. arch. biol. technol.

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The aim of this study was to induce and analyze embryogenic calli from two types of explants (leaves and meristems) of the hybrid Eucalyptus grandis x Eucalyptus urophylla. Leaves and meristems of plants kept in a nursery were disinfected and inoculated in Petri dishes containing MS culture medium supplemented with different concentrations of the growth regulator dicamba (1.13, 4.52, and 9.04 µM) and without it. At 60 days of culturing, the calli were analyzed by scanning electron microscopy and at 90 days were evaluated by light microscopy in regard to the embryogenic characteristics of the cells. Different type of calli were induced in leaf explants, designated as Type I with light yellow coloring, Type II with dark yellow coloring, and Type III of brown coloring; however, only Type I had embryogenic characteristics. In the meristematic explants, only one type of callus was induced, and it had embryogenic characteristics. At 90 days of culturing, the formation of somatic embryos in the different embryogenic stages was observed and the formation of procambium, protoderm, and ground meristem tissues. At 150 days of culturing, the concentration of 1.13 µM of dicamba was prominent in the formation of somatic embryos in the different embryogenic stages.

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Efeito dos reguladores de crescimento dicamba e picloram na embriogênese somática em Eucalyptus grandis

Cited by 11 publications

References 22 publications

Embryogenic potential of the callus of gabirobeira, Campomanesia adamantium (Cambess) O. Berg

Embryogenic potential of the callus of gabirobeira, Campomanesia adamantium (Cambess) O. Berg

Induction of somatic embryogenesis and plant regeneration in the tropical timber tree Spanish red cedar [Cedrela odorata L. (Meliaceae)]

Morpho and Cytological Differentiation of Calli of Eucalyptus grandis x Eucalyptus urophylla During Somatic Embryogenesis

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