Efeito de Saccharomyces cerevisiae na síntese de fitoalexinas em sorgo, na germinação e formação de apressórios por fungos fitopatogênicos e na proteção de pepino a Colletotrichum lagenarium e sorgo a Colletotrichum sublineolum.

Bonaldo, Solange Maria

doi:10.11606/t.11.2005.tde-16062005-135554

Cited by 6 publications

(7 citation statements)

References 109 publications

(100 reference statements)

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“…Although, Bonaldo et al (2007) found that when the crude extract of eucalyptus was diluted, even so, there was induction of the phytoalexins, deoxiantocianidine in sorghum mesocotyls. Bonaldo (2005) also observed that the dilutions 10, 100, 1,000 and 10,000 times of preparation of S. cerevisiae induced phytoalexins in sorghum mesocotyls.…”

Section: Among the Methods Deployed On The Moleculementioning

confidence: 70%

Plant defense elicitors purification in soybean and bean from pathogenic nematode

GonÃ§alves-Trevisoli¹,

Stangarlin²,

Rissato³

et al. 2016

Afr. J. Agric. Res.

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) was used as induction standard treatment. Ion exchange chromatography (IEC) and gel filtration chromatography (GFC) were applied to separate power eliciting fractions from five hundred female root knot nematodes (Meloidogyne javanica). The purification of elicitors, through IEC, resulted in sixty glicidic fractions and six glycoprotein ones. They were classified according to their nature, being twenty-six glicidic fractions and thirty-seven glycoprotein ones, with molecular masses ranging from 29.19 to 2,989.25 kDa. From the purified fractions, eight of them presented phaseolin inducing effect potential, whereas fifteen fractions presented gliceolin inducing effect. Chromatography proved to be efficient in purifying the eliciting compounds. Compounds having gliceolin and phaseolin suppressing characteristics were verified in the bioassays. For those fractions obtained through IEC and posteriorly submitted to GFC that did not induce phytoalexin, it is suggested that the molecules need to act jointly so there is eliciting effect and thus induce a defense response in the plant.

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Section: Among the Methods Deployed On The Moleculementioning

confidence: 70%

Plant defense elicitors purification in soybean and bean from pathogenic nematode

GonÃ§alves-Trevisoli¹,

Stangarlin²,

Rissato³

et al. 2016

Afr. J. Agric. Res.

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“…Para a extração das moléculas eliciadoras de S. cerevisiae, foi utilizada levedura de panificação (fermento biológico Fleischmann, AB Brasil Indústria e Comércio de Alimentos Ltda., Jundiaí, SP), em tabletes de 200 g dissolvidos em 1 L de água destilada (Wulff & Pascholati, 1999). Para melhorar a extração e solubilização das moléculas eliciadoras, a levedura foi submetida à autoclavagem a 121°C, conforme Bonaldo (2005). A suspensão de células de S. cerevisiae foi autoclavada em quatro etapas, com intervalos de tempo diferentes.…”

Section: Methodsunclassified

Resistência de plântulas de pepineiro a Colletotrichum lagenarium induzida por frações de extrato de Saccharomyces cerevisiae

Zanardo

Pascholati

Fialho

2009

Pesq. agropec. bras.

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Resumo -O objetivo deste trabalho foi purificar frações do extrato de Saccharomyces cerevisiae que induzam a resistência do pepineiro a Colletorichum lagenarium e determinar sua eficiência na proteção de plântulas, em casa de vegetação. A suspensão de células da levedura, seus extratos brutos aquosos autoclavados por 4 e 10 horas, duas frações (sobrenadante e precipitado) resultantes da precipitação etanólica do extrato bruto (4 horas) e as frações do sobrenadante identificadas na cromatografia de troca aniônica foram aplicados a cotilédones de plântulas de pepino. A suspensão de células de levedura não apresentou controle significativo da doença. Os extratos brutos autoclavados reduziram a severidade da antracnose entre 82 a 86% e as frações resultantes da precipitação etanólica em 98%. As frações do sobrenadante não ligadas à resina DEAE-celulose (pico I da cromatografia) ou ligadas à resina (pico II) -constituídas principalmente por carboidratos -foram as mais efetivas na indução de resistência nas plântulas e reduziram a severidade da antracnose em 81 e 72%, respectivamente. A autoclavagem de extratos brutos aquosos de S. cerevisiae é necessária para a extração de moléculas eliciadoras da resistência a C. lagenarium, em cotilédones de pepineiro.Termos para indexação: carboidratos, eliciadores, extrato de levedura, indução de resistência, patógeno. Resistance of cucumber seedlings to Colletotrichum lagenarium induced by fractions of Saccharomyces cerevisiae extractAbstract -The objective of this work was to purify the Saccharomyces Cerevisiae extract fractions that induce resistance to Colletorichum lagenarium in cucumber and to determine their efficiency in protecting seedlings in the greenhouse. Yeast cell suspension, its crude aqueous extract autoclaved for 4 and 10 hours, two fractions (supernatant and pellet) resulting from ethanolic precipitation of the crude extract (4 hours) and the supernatant fractions identified in anion exchange chromatography were sprayed onto cucumber cotyledons. The yeast cell suspension did not significantly control the disease. The autoclaved crude extracts reduced the anthracnose severity between 82 and 86%, and the ethanolic precipitation fractions in 98%. The supernatant fractions non-adsorbed (chromatography peak I) and adsorbed (peak II) to DEAE-cellulose resin, containing mainly carbohydrates, were the most effective for inducing assistance to the seedlings, and reduced the anthracnose severity in 81 and 72% respectively. Autoclaving crude extracts of S. cerevisiae is necessary to extract molecules that elicit resistance to C. lagenarium in cucumber cotyledon.

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“…The seedlings were kept under fluorescent light at 25 ± 1°C for 60 h. Subsequently, the mesocotyls were removed, excised, weighed and placed in microcentrifuge tubes containing 1.4 mL of acidified 80% methanol (0.1% HCL; v/v). These were kept cooled to 4 ± 1°C per 96 h for the extraction of the pigments (Bonaldo, 2005). The absorbance was read at 480 nm (BioSpectro model SP-220) (Nicholson et al, 1988;Stangarlin et al, 2010).…”

Section: Phytoalexin Induction In Sorghum Mesocotylsmentioning

confidence: 99%

Enzymatic activity and elicitor of phytoalexins of Lippia sidoides Cham. and endophytic fungi

Ferreira¹,

Veloso²,

Santos³

et al. 2018

Afr. J. Biotechnol.

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Efeito de Saccharomyces cerevisiae na síntese de fitoalexinas em sorgo, na germinação e formação de apressórios por fungos fitopatogênicos e na proteção de pepino a Colletotrichum lagenarium e sorgo a Colletotrichum sublineolum.

Cited by 6 publications

References 109 publications

Plant defense elicitors purification in soybean and bean from pathogenic nematode

Plant defense elicitors purification in soybean and bean from pathogenic nematode

Resistência de plântulas de pepineiro a Colletotrichum lagenarium induzida por frações de extrato de Saccharomyces cerevisiae

Enzymatic activity and elicitor of phytoalexins of Lippia sidoides Cham. and endophytic fungi

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