Efeito da temperatura sobre o crescimento e esporulação in vitro de fungos hiperparasitas de Asperisporium caricae

Vivas, Janieli Maganha Silva; Vivas, Marcelo; Silveira, Silvaldo Felipe da

doi:10.1590/1983-40632015v4529774

Cited by 8 publications

(7 citation statements)

References 15 publications

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“…Sporulation was strongly influenced by temperature, being higher at 20±1 °C and decreasing as temperature increased. Vivas et al (2015) showed that isolates of both Hansfordia pulvinata (Berk. & Curtis) Hughes and Acremonium spp.…”

Section: Discussionmentioning

confidence: 99%

Culture Medium, Light Regime and Temperature Affect the Development of Sirosporium diffusum

Poletto¹,

Muniz²,

Fantinel³

et al. 2018

JAS

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Sirosporium diffusum is the causal agent of the brown leaf spot disease on pecan trees that seriously damages the foliage of adult plants and seedlings. This fungal species is difficult to grow satisfactorily in a culture medium. Therefore, the aim of this study was to evaluate the effects of different physical conditions on the development of S. diffusum. In the first assay, eight culture media and five light regimes were combined, while in the second, the three treatments that promoted highest sporulation were combined with three temperatures. The trials were conducted in a two-factorial arrangement in a fully randomized design with six replicates. V8, V8CaCO 3, and CA media under a 24-h photoperiod produced the highest respective sporulations: 29 × 10 4 , 35 × 10 4 , and 41 × 10 4 conidia ml -1. The best temperature for sporulation was 20±1 °C for all culture media, especially V8CaCO 3 and CA. The best artificial conditions for obtaining good mycelial growth and sporulation consisted of a photoperiod of 24 h, temperature of 20±1 °C and V8CaCO 3 or CA culture medium.

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Section: Discussionmentioning

confidence: 99%

Culture Medium, Light Regime and Temperature Affect the Development of Sirosporium diffusum

Poletto¹,

Muniz²,

Fantinel³

et al. 2018

JAS

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“…Three isolates of Acremonium (A-598, A-602, and A-617) and one of H. pulvinata (H-611), previously obtained by direct isolation from lesions of papaya black spot with signs of hyperparasites were used (Vivas et al, 2015). The monosporic cultures were preserved in tubes, under refrigeration at 10 °C, transferred to Petri dishes containing PDA, and placed in BOD incubator at 25 ± 0.5 °C, under a 12-hour photoperiod for 12 days.…”

Section: Hyperparasites and Alternative Products Usedmentioning

confidence: 99%

“…The mycelial growth was measured, with a transparent ruler, on the external part on the bottom of the dish, after 15 days of incubation, when the radial growth of the colonies was measured in two orthogonal directions to, then, obtain the mean of both measures. Discs with 7-mm diameter were removed from the median area of the colonies using a cork punch to evaluate the sporulation, according to the methodology described by Vivas et al (2015). The discs were transferred to an assay tube containing 1 mL of water with 0.3% of Tween at 20%.…”

Section: Effect Of Different Products On Mycelial Growth Sporulationmentioning

confidence: 99%

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Sensitivity of hyperparasitic fungi to alternative products for use in the control of papaya black spot

et al. 2021

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The use of more than one control technique can maximize the reduction of the damages caused by the fungus Asperisporium caricae causal agent of the black spot in the papaya crop. The objective of this work was to evaluate the sensitivity of the fungi Hansfordia pulvinata and Acremonium spp. to alternative products with potential for use in the control of the black-spotted ptarmigan. Three isolates of Acremonium spp. (A-598, A-602 and A-617) and an isolate of H. pulvinata (H-611) were grown in BDA medium containing Agro-Mos  , Bion  , Ecolife  , Hortifospk  , Matriz G  , Vitaphol  separately. The Amistar 500WG  fungicide was used as a positive control and pure BDA as a negative control. The toxicity of the tested products was determined based on the values of the biological index, derived from the means of mycelial growth, sporulation and germination of conidia, in each experimental unit. In this way it was possible to select the products classified as compatible for all isolates, and to test them in vivo. In the greenhouse, only the isolates and isolates with selected products, were applied in papaya plants with foliar symptoms of black-spotted. The incidence of leaves with hyperparasites and the percentage of black-painted lesions colonized by the tested isolates were evaluated. Thus, the H-611 isolate proved to be compatible with most of the alternative products tested, except with Hortifos  . Bion  and Matrix  products were compatible with all tested isolates and could be used in conjunction with Acremonium spp. and H. pulvinata to control the papaya black spot, since these products did not present toxicity on the hyperparasitic fungi.

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“…Although there are fungi with potential of black spot biocontrol (Vivas et al, 2015), scarce information is available on the mode of action of the antagonistic fungi on A. caricae, specifically in papaya culture. Researches that test the antagonistic potential are important to support the development of biological control programs for papaya black spot.…”

Section: Introductionmentioning

confidence: 99%

Antagonism of fungi with biocontrol potential of papaya black spot caused by Asperisporium caricae

Vivas¹,

Silveira²,

Santos³

et al. 2018

Aust J Crop Sci

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The antifungal activity of fungi with biocontrol potentials should be studied. Therefore, this study aimed at establishing alternative agent to replace the chemical control of papaya black spot. We investigated the antagonistic activity of the fungi Hansfordia pulvinata (H-611), Acremonium sp (A-602, A-617 and A-598), Simplicillium lanosoniveum (S-599), Lecanicillium lecanii (L-622), and Sarocladium implicatum (I-609) on the phytopathogenic fungus A. caricae, by evaluating the mycoparasitism, the enzymatic activity and the production of volatile and non-volatile compounds. For the evaluation of mycoparasitism, each antagonic fungi isolate was microcultured along with conidia of A. caricae. Then, events of mycoparasitism were evaluated at 24h, 48h, 72h, and 96h after inoculation under an optical microscope. We estimated the enzymatic activity (protease and lipase) of the fungi in a particular culture medium for each enzyme. The activity of the protease was reflected in the mean diameter of the halo. The lipase activity was measured by the halo/colony relation. In order to detect volatile compounds in the antibiosis test, A. caricae was cultured under a medium along with the isolates described. For the test of non-volatile compounds, we evaluated the germination of A. caricae in culture medium by diffusion under cellophane paper. As a result, the mycoparasitism of the H-611, A-602, A-617, A-598, S-599, and L-622 isolates was confirmed on the microcultures. We observed higher activities of protease in the I-609, S-599, A-602, and A-598 isolates. The H-611 and A-602 isolates presented higher activities for lipase. We did not observe effect of volatile compounds in any of the tested isolates. In the non-volatile compound test, there was production of antifungal metabolite highlighting the A-617 isolate, which inhibited the germination of the conidia of A. cariae by 100%.

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Efeito da temperatura sobre o crescimento e esporulação in vitro de fungos hiperparasitas de Asperisporium caricae

Cited by 8 publications

References 15 publications

Culture Medium, Light Regime and Temperature Affect the Development of Sirosporium diffusum

Culture Medium, Light Regime and Temperature Affect the Development of Sirosporium diffusum

Sensitivity of hyperparasitic fungi to alternative products for use in the control of papaya black spot

Antagonism of fungi with biocontrol potential of papaya black spot caused by Asperisporium caricae

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