Efeito anestésico do óleo de cravo em alevinos de lambari

Silva, Elyara Maria Pereira-da; Oliveira, Ricardo Henrique Franco de; Ribeiro, Maria Angélica Rosa; Coppola, Milena Pereira

doi:10.1590/s0103-84782009005000127

Cited by 24 publications

(5 citation statements)

References 21 publications

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“…The concentration of the anesthetic will depend on whether the fish is to be anaesthetized or euthanized, according to the goals of the study (Silva et al, 2009). Smaller fishes respond to lower concentrations than larger fishes, thus the effect of clove oil will vary depending on the fish species and their sizes (Woody et al, 2002;Ross and Ross, 2008).…”

Section: Introductionmentioning

confidence: 99%

The efficacy of clove oil as an anaesthetic and in euthanasia procedure for small-sized tropical fishes

et al. 2016

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Clove oil is used as a fish anesthetic because it is a natural and inexpensive product with low toxicity risks. The goal of the present study was to determine the appropriate concentration of clove oil for small-sized tropical fish to be used in mark-recapture studies or when individuals are to be sacrificed. We applied three different clove oil concentrations (D1=0.05 mL, D2=0.10 mL and D3=0.20 mL per 500 mL of water) on three small-sized fish species. We found a negative relationship between induction time and treatment for two species (Hyphessobrycon sp.1 and Hemigrammus sp.), while concentration was unrelated to recovery time. Fish body length was positively related to induction time in the D2 treatment for Hemigrammus sp., and negatively for Hyphessobrycon sp.1 in the D1 treatment, but was unrelated to recovery time for three species and treatments. Mortality rates varied across treatments, but higher rates were observed with higher clove oil concentrations. We conclude that 0.05 mL of clove oil per 500 mL of water is the most efficient dose for studies where fish will be released back to their natural habitats, while 0.20 mL of clove oil is recommended for studies that require fish euthanization for further laboratory analyses.Keywords: Eugenol, anesthesia, euthanasia, field experiment, fish manipulation.A eficiência do óleo de cravo como anestésico e em procedimentos de eutanaisa em peixes de pequeno porte Resumo O óleo de cravo é recomendado como anestésico para peixes por ser produto de origem natural, baixo custo e apresentar poucos riscos de intoxicação. O objetivo deste trabalho foi determinar concentrações adequadas de óleo de cravo para anestesiar ou eutanasiar peixes de pequeno porte em ambiente natural. Foram testadas três concentrações do anestésico (D1=0,05 mL, D2=0,10 mL e D3=0,20 mL) em três espécies de peixes de pequeno. Houve uma relação negativa entre o tempo para a sedação dos indivíduos e a concentração para duas espécies (Hyphessobrycon sp.1 e Hemigrammus sp.), porém não foi encontrada relação entre o tempo para recuperação e as concentrações. Os exemplares maiores de Hemigrammus sp. levaram mais tempo para serem sedados no tratamento D2, já o contrário foi observado para Hyphessobrycon sp.1 no tratamento D1, enquanto que não houve efeito do comprimento no tempo de recuperação das três espécies. A mortalidade dos indivíduos variou entre as três concentrações do anestésico e as maiores taxas de mortalidade ocorreram nas maiores concentrações. Desse modo, a concentração de 0,05 mL é eficiente para estudos que envolvem manuseio e a soltura dos peixes, enquanto que a concentração de 0,20 mL é recomendada em estudos onde os peixes precisam ser sacrificados.Palavras-chave: Eugenol, anestesia, eutanásia, experimento de campo, manipulação de peixes.

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Section: Introductionmentioning

confidence: 99%

The efficacy of clove oil as an anaesthetic and in euthanasia procedure for small-sized tropical fishes

et al. 2016

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“…Thus, one way to improve fish management is to minimize stress, preserving well-being. To achieve this goal, the use of anesthetics and/or sedatives has been suggested in fish farms [14].…”

Section: Introductionmentioning

confidence: 99%

Effects of anesthetic MS-222 on stress and reproduction of South American silver catfish (Rhamdia quelen) males

Teixeira

Marques

Rodrigues

et al. 2021

Animal Reproduction Science

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“…Interest in growing and cultivating Astyanax spp. has increased, but few essential oils with anaesthetic properties have been evaluated for use in this species (Ostrensky, Pedrazzani, & Vicente, ; Pereira‐da‐Silva, de Oliveira, Ribeiro, & Coppola, ; Pereira‐da‐Silva, de Oliveira, & Nero, ). Therefore, this study aimed to determine the lowest effective concentration (LEC) of 2‐phenoxyethanol and essential oils of M. alternifolia and O. gratissimum able to induce anaesthesia in A. bimaculatus .…”

Section: Introductionmentioning

confidence: 99%

Assessment of induction and recovery times of anaesthesia in Astyanax bimaculatus using 2‐phenoxyethanol and the essential oils of Melaleuca alternifolia and Ocimum gratissimum

et al. 2019

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Anaesthetics are widely used to reduce stress, excessive movement and perception to external stimuli associated with aquaculture procedures (Inoue, Afonso, Iwama, & Moraes, 2005). As a result, since 1930 (Hoskonen & Pirhonen, 2004), anaesthetics have been used in fish to facilitate management and reduce stress (Ross & Ross, 2008), depressing the central nervous system (CNS) and reducing sensory perception, presumably increasing the welfare of fish when manipulated (Carter, Woodley, & Brown, 2011;Zahl, Samuelsen, & Kiessling, 2012). In fish, the anaesthetic is given by immersion in anaesthetic solution (Mello et al., 2012). It is absorbed by the gills and diffused into the blood, leading to the CNS (Ross & Ross, 2008). The 2-phenoxyethanol (2-Phe) (Ethylene glycol monophenyl ether) is a colourless, oily and aromatic liquid. It is believed that this agent inhibits the activity of NMDA (N-methyl-D-aspartate) glutamate receptors, reducing pain (Musshoff, Madeja, Binding, Witting, & Speckmann, 1999). Activation of the NMDA receptors increases the excitability of the neurons, resulting in the opening of a nonselective ion channel for the cations, allowing the flow of sodium and calcium into the interior of the cell and the flow of potassium to the exterior (Priborsky & Velisek, 2018). Inhibition of the NMDA receptor has been associated with an analgesic effect (Grasshoff, Drexler, Rudolph, & Antkowiak, 2006). The 2-Phe is considered suitable for use in aquaculture because of its ease of preparation, low cost, quick action and uncomplicated recovery (Pucea, Garin, & Freminet, 1989); it also presents bactericidal and fungicidal properties (Jolly, Mawdesley, & Bucke, 1972).As alternatives, essential oils (EOs) can replace synthetic products. Belonging to the Myrtaceae family, Melaleuca alternifolia has shown itself to be a good anaesthetic for use in fish (Correia et al., 2018;Hajek, 2011). Known as tea tree and widely used in traditional medicine for anaesthetic and analgesic properties, this tree, which is native to Australia, also has antifungal, antibiotic and anti-inflammatory characteristics, having terpinen-4-ol and γ-terpinene as main components (Carson,

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Efeito anestésico do óleo de cravo em alevinos de lambari

Abstract: RESUMO Para avaliar o efeito anestésico do óleo de cravo em lambaris (Astyanax altiparanae), cinco grupos de 30 alevinos (0,6±0,1g)

Cited by 24 publications

References 21 publications

The efficacy of clove oil as an anaesthetic and in euthanasia procedure for small-sized tropical fishes

The efficacy of clove oil as an anaesthetic and in euthanasia procedure for small-sized tropical fishes

Effects of anesthetic MS-222 on stress and reproduction of South American silver catfish (Rhamdia quelen) males

Assessment of induction and recovery times of anaesthesia in Astyanax bimaculatus using 2‐phenoxyethanol and the essential oils of Melaleuca alternifolia and Ocimum gratissimum

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