Ectopic expression of WOX5 promotes cytokinin signaling and de novo shoot regeneration

Lee, Kyoung-Hee; Kim, Jong Hee; Park, Ok-Sun; Jung, Yu Jin; Seo, Pil Joon

doi:10.1007/s00299-022-02932-4

Cited by 12 publications

(8 citation statements)

References 19 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Complementarily, the transcript level of ARR5 was higher in the wox5 mutant on both CIM and SIM (Zhai and Xu, 2021;Lee et al, 2022). The loss of ARR7 function resulted in strong induction of callus formation, which points to an inhibitory role for ARR7 in the acquisition of pluripotency (Buechel et al, 2010).…”

Section: Acquisition Of Meristematic Identitymentioning

confidence: 91%

Cytokinins – regulators of de novo shoot organogenesis

Šmeringai,

Schrumpfová,

Pernisová

2023

Front. Plant Sci.

View full text Add to dashboard Cite

Plants, unlike animals, possess a unique developmental plasticity, that allows them to adapt to changing environmental conditions. A fundamental aspect of this plasticity is their ability to undergo postembryonic de novo organogenesis. This requires the presence of regulators that trigger and mediate specific spatiotemporal changes in developmental programs. The phytohormone cytokinin has been known as a principal regulator of plant development for more than six decades. In de novo shoot organogenesis and in vitro shoot regeneration, cytokinins are the prime candidates for the signal that determines shoot identity. Both processes of de novo shoot apical meristem development are accompanied by changes in gene expression, cell fate reprogramming, and the switching-on of the shoot-specific homeodomain regulator, WUSCHEL. Current understanding about the role of cytokinins in the shoot regeneration will be discussed.

show abstract

Section: Acquisition Of Meristematic Identitymentioning

confidence: 91%

Cytokinins – regulators of de novo shoot organogenesis

Šmeringai,

Schrumpfová,

Pernisová

2023

Front. Plant Sci.

View full text Add to dashboard Cite

show abstract

“…Showing that this set of genes is essential for plant regeneration, ectopic WUS expression might, therefore, be a relevant strategy for enhanced plant regeneration. For further details see [ 11 ].…”

Section: Major Aspects Of Plant Regenerationmentioning

confidence: 99%

Plant Growth Regulation in Cell and Tissue Culture In Vitro

Pasternak,

Steinmacher

2024

Plants

View full text Add to dashboard Cite

Precise knowledge of all aspects controlling plant tissue culture and in vitro plant regeneration is crucial for plant biotechnologists and their correlated industry, as there is increasing demand for this scientific knowledge, resulting in more productive and resilient plants in the field. However, the development and application of cell and tissue culture techniques are usually based on empirical studies, although some data-driven models are available. Overall, the success of plant tissue culture is dependent on several factors such as available nutrients, endogenous auxin synthesis, organic compounds, and environment conditions. In this review, the most important aspects are described one by one, with some practical recommendations based on basic research in plant physiology and sharing our practical experience from over 20 years of research in this field. The main aim is to help new plant biotechnologists and increase the impact of the plant tissue culture industry worldwide.

show abstract

“…Various morphogenesis pathways were revealed in calli in the subsequent stages of in vitro cultivation (Figure 1(4-11)): organogenesis (gemmogenesis-type or caulogenesistype, consisting of the formation and development of the shoot; rhizogenesis-type, consisting of the formation and development of the root; or gemmorhizogenesis-type, consisting of the formation and development of the gemmorhizogenic structure that combines both the shoot and the root) and somatic embryogenesis (consisting of the formation and development of somatic embryos) (reviews [15,16,21,33]). Thus, reprogrammed callus cells exhibit the properties of pluripotency and totipotence under adequate in vitro conditions.…”

Section: Histological Approach To the Evaluation Of In Vitro Morphoge...mentioning

confidence: 99%

“…Morphogenesis pathways lead to the regeneration of full-fledged plants under further favorable in vitro conditions. The plant regeneration system in calli is called "de novo plant regeneration" [9,16], "in vitro plant regeneration" [15] or "indirect plant regeneration" [43].…”

Section: Histological Approach To the Evaluation Of In Vitro Morphoge...mentioning

confidence: 99%

“…A large number of experimental works have been published concerning the study of both in vitro callus formation and the morphogenesis events in them that lead to the formation of regenerants. Especially great success has been achieved in identifying the molecular aspects of these processes, namely the participation of a number of specific gene systems in them [9,[11][12][13][14][15]. Important theoretical generalizations have been made.…”

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

Histological Approach to the Study of Morphogenesis in Callus Cultures In Vitro: A Review

Круглова

Zinatullina

Yegorova

2023

IJPB

View full text Add to dashboard Cite

The use of in vitro callus cultures as experimental model systems allows us to get closer to understanding the patterns and features of morphogenesis in intact plants. In this regard, the problem of realizing the morphogenetic potential of callus cells due to their pluri- and totipotency properties is of great interest. To solve this problem, it is important to use the histological approach, which involves studying the structures of developing tissues, organs and organisms in their interactions and relationships. This review article analyzes data devoted to the study of the histological features of formed primary morphogenic calli (formation of morphogenetic centers and superficial meristematic zones), as well as the in vitro morphogenesis pathways in calli that lead to the formation of regenerants (de novo organogenesis and in vitro somatic embryogenesis). The terminology used is considered. Some questions for discussion are raised. The opinion is expressed that histological (structural) studies should be considered as a methodologic basis for further investigation of various morphogenetic scenarios in in vitro callus cultures, especially in economically valuable plants and for biotechnological purposes.

show abstract

Ectopic expression of WOX5 promotes cytokinin signaling and de novo shoot regeneration

Cited by 12 publications

References 19 publications

Cytokinins – regulators of de novo shoot organogenesis

Cytokinins – regulators of de novo shoot organogenesis

Plant Growth Regulation in Cell and Tissue Culture In Vitro

Histological Approach to the Study of Morphogenesis in Callus Cultures In Vitro: A Review

Contact Info

Product

Resources

About