Cellular dedifferentiation, the transition of differentiated somatic cells to pluripotent stem cells, ensures developmental plasticity and contributes to wound healing in plants. Wounding induces cells to form a mass of unorganized pluripotent cells called callus at the wound site. Explanted cells can also form callus tissues in vitro. Reversible cellular differentiation-dedifferentiation processes in higher eukaryotes are controlled mainly by chromatin modifications. We demonstrate that ARABIDOPSIS TRITHORAX-RELATED 2 (ATXR2), a histone lysine methyltransferase that promotes the accumulation of histone H3 proteins that are trimethylated on lysine 36 (H3K36me3) during callus formation, promotes early stages of cellular dedifferentiation through activation of () genes. The genes of are activated during cellular dedifferentiation to enhance the formation of callus. Leaf explants from mutants exhibited a reduced ability to form callus and a substantial reduction in gene expression. ATXR2 bound to the promoters of genes and was required for the deposition of H3K36me3 at these promoters. ATXR2 was recruited to promoters by the transcription factors AUXIN RESPONSE FACTOR 7 (ARF7) and ARF19. Leaf explants from double mutants were defective in callus formation and showed reduced H3K36me3 accumulation at promoters. Genetic analysis provided further support that ARF7 and ARF19 were required for the ability of ATXR2 to promote the expression of genes. These observations indicate that the ATXR2-ARF-LBD axis is key for the epigenetic regulation of callus formation in.
Massive machine-type communication (mMTC) and ultrareliable and low-latency communication (URLLC) are two key service types in the fifth-generation (5G) communication systems, pursuing scalability and reliability with low-latency, respectively. These two extreme services are envisaged to agglomerate together into critical mMTC shortly with emerging use cases (e.g., wide-area disaster monitoring, wireless factory automation), creating new challenges to designing wireless systems beyond 5G. While conventional network slicing is effective in supporting a simple mixture of mMTC and URLLC, it is difficult to simultaneously guarantee the reliability, latency, and scalability requirements of critical mMTC (e.g., < 4ms latency, 106 devices/km 2 for factory automation) with limited radio resources. Furthermore, recently proposed solutions to scalable URLLC (e.g., machine learning aided URLLC for driverless vehicles) are ill-suited to critical mMTC whose machine type users have extremely limited energy budget and computing capability that should be tightly optimized for given tasks. In view of this, this paper aims to characterize promising use cases of critical mMTC and search for their possible solutions. To this end, we first review the state-of-theart (SOTA) technologies for separate mMTC and URLLC services and then identify key challenges from conflicting SOTA requirements, followed by potential approaches to prospective critical mMTC solutions at different layers. Index Terms-Ultra reliable low latency communication (URLLC), massive machine type communications (mMTC), critical mMTC, 5G, beyond 5G.
Plant somatic cells can be reprogrammed by in vitro tissue culture methods, and massive genome-wide chromatin remodeling occurs, particularly during callus formation. Since callus tissue resembles root primordium, conversion of tissue identity is essentially required when leaf explants are used. Consistent with the fact that the differentiation state is defined by chromatin structure, which permits limited gene profiles, epigenetic changes underlie cellular reprogramming for changes to tissue identity. Although a histone methylation process suppressing leaf identity during leaf-to-callus transition has been demonstrated, the epigenetic factor involved in activation of root identity remains elusive. Here, we report that JUMONJI C DOMAIN-CONTAINING PROTEIN 30 (JMJ30) stimulates callus formation by promoting expression of a subset of LATERAL ORGAN BOUNDARIES-DOMAIN (LBD) genes that establish root primordia. The JMJ30 protein binds to promoters of the LBD16 and LBD29 genes along with AUXIN RESPONSE FACTOR 7 (ARF7) and ARF19 and activates LBD expression. Consistently, the JMJ30-deficient mutant displays reduced callus formation with low LBD transcript levels. The ARF-JMJ30 complex catalyzes the removal of methyl groups from H3K9me3, especially at the LBD16 and LBD29 loci to activate their expression during leaf-to-callus transition. Moreover, the ARF-JMJ30 complex further recruits ARABIDOPSIS TRITHORAX-RELATED 2 (ATXR2), which promotes deposition of H3K36me3 at the LBD16 and LBD29 promoters, and the tripartite complex ensures stable LBD activation during callus formation. These results indicate that the coordinated epigenetic modifications promote callus formation by establishing root primordium identity.
The recently introduced 5G New Radio is the first wireless standard natively designed to support critical and massive machine type communications (MTC). However, it is already becoming evident that some of the more demanding requirements for MTC cannot be fully supported by 5G networks. Alongside, emerging use cases and applications towards 2030 will give rise to new and more stringent requirements on wireless connectivity in general and MTC in particular. Next generation wireless networks, namely 6G, should therefore be an agile and efficient convergent network designed to meet the diverse and challenging requirements anticipated by 2030. This paper explores the main drivers and requirements of MTC towards 6G, and discusses a wide variety of enabling technologies. More specifically, we first explore the emerging key performance indicators for MTC in 6G. Thereafter, we present a vision for an MTC-optimized holistic end-to-end network architecture. Finally, key enablers towards (1) ultra-low power MTC, (2) massively scalable global connectivity, (3) critical and dependable MTC, and (4) security and privacy preserving schemes for MTC are detailed. Our main objective is to present a set of research directions considering different aspects for an MTC-optimized 6G network in the 2030-era.
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