Ectopic expression of wheat <i><scp>TaCIPK14</scp></i>, encoding a calcineurin B‐like protein‐interacting protein kinase, confers salinity and cold tolerance in tobacco

Deng, Xianwen; Zhou, Shiyi; Hu, Wei; Feng, Jialu; Zhang, Fan; Huang, Chao; Luo, Qingchen; He, Yanzhen; Yang, Guangxiao; He, Guangyuan

doi:10.1111/ppl.12046

Cited by 74 publications

(49 citation statements)

References 55 publications

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“…The results of these two studies reveal that during cold exposure some proteins are phosphorylated by protein kinases, whereas others are dephosphorylated by protein phosphatases. Presently, many different kinases or phosphatases under cold stress had been identified, such as Calcium dependent protein kinases (CDPK)16, Ca 2+ /calmodulin-dependent protein kinases (CAMK)17, MAPK (mitogen activated protein kinase) module718, STY kinases19, CBL-interacting protein kinase (CIPK)2021, OST122 and AP2C1 proteins23. Phosphorylation changes of MAPK cascades in plant cold resistance have been well reported, particularly in model plants.…”

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confidence: 99%

Comparative Phosphoproteomics Reveals an Important Role of MKK2 in Banana (Musa spp.) Cold Signal Network

Gao

Zhang

et al. 2017

Sci Rep

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Low temperature is one of the key environmental stresses, which greatly affects global banana production. However, little is known about the global phosphoproteomes in Musa spp. and their regulatory roles in response to cold stress. In this study, we conducted a comparative phosphoproteomic profiling of cold-sensitive Cavendish Banana and relatively cold tolerant Dajiao under cold stress. Phosphopeptide abundances of five phosphoproteins involved in MKK2 interaction network, including MKK2, HY5, CaSR, STN7 and kinesin-like protein, show a remarkable difference between Cavendish Banana and Dajiao in response to cold stress. Western blotting of MKK2 protein and its T31 phosphorylated peptide verified the phosphoproteomic results of increased T31 phosphopeptide abundance with decreased MKK2 abundance in Daojiao for a time course of cold stress. Meanwhile increased expression of MKK2 with no detectable T31 phosphorylation was found in Cavendish Banana. These results suggest that the MKK2 pathway in Dajiao, along with other cold-specific phosphoproteins, appears to be associated with the molecular mechanisms of high tolerance to cold stress in Dajiao. The results also provide new evidence that the signaling pathway of cellular MKK2 phosphorylation plays an important role in abiotic stress tolerance that likely serves as a universal plant cold tolerance mechanism.

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confidence: 99%

Comparative Phosphoproteomics Reveals an Important Role of MKK2 in Banana (Musa spp.) Cold Signal Network

Gao

Zhang

et al. 2017

Sci Rep

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“…Though more than 29 CIPK genes have been found in wheat, only a few of them had been functionally analyzed, such as TaCIPK14 [15], TaCIPK19 ( WPK 4) [28], TaCIPK24 [7] and TaCIPK29 [29]. In this study, we found that TaCIPK2 was up-regulated in wheat leaves by PEG, H 2 O 2 , and ABA treatments (Fig 3B–3D), implying a potential role of TaCIPK2 in stress responses.…”

Section: Discussionmentioning

confidence: 99%

“…The AtCBL4-CIPK24 (SOS3-SOS2) complex specifically regulates Na + efflux by the Na + /H + antiporter and enhances salt tolerance [10, 11]. The overexpression of many CIPK genes increases salt tolerance in plants, including ZmCIPK21 in maize [12], HbCIPK2 in Hordeum brevisubulatum [13], PeCIPK24-26 in Populus species [14], and TaCIPK14 in wheat [15]. These genes are involved in the salt overly sensitive (SOS) signaling pathway.…”

Section: Introductionmentioning

confidence: 99%

A CBL-Interacting Protein Kinase TaCIPK2 Confers Drought Tolerance in Transgenic Tobacco Plants through Regulating the Stomatal Movement

Wang

Sun

et al. 2016

PLoS ONE

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In plants, the CBL-CIPK signaling pathways play key roles in the response to abiotic stresses. However, functional studies of CIPKs in the important staple crop wheat are very rare. In this study, we identified a CIPK gene from wheat, designated TaCIPK2. Expression analysis results showed that TaCIPK2 could be up-regulated in wheat leaves by polyethylene glycol, abscisic acid and H2O2 treatments. Subcellular localization analyses revealed that TaCIPK2 was present in whole wheat epidermal cells. A yeast two-hybrid assay indicated that TaCIPK2 interacted with TaCBL1, 2, 3 and 4 in vitro. Transgenic tobacco plants over-expressing TaCIPK2 exhibited increased drought tolerance, indicated by a larger proportion of green cotyledons and higher survival rates under the osmotic and drought stress conditions compared with control plants. Additionally, physiological index analyses revealed that the transgenic tobacco plants had lower water loss rates and ion leakage, accumulated less malondialdehyde and H2O2, and had higher catalase and superoxide dismutase activities than the control plants. The transgenic plants also exhibited faster stomatal closure following exposure to osmotic stress conditions. The seed germination rates and stomatal aperture of TaCIPK2-overexpressing tobacco plants decreased after exogenous abscisic acid treatment was applied, implying that the transgenic tobacco plants were more sensitive to exogenous abscisic acid than the control plants. Our results indicate that TaCIPK2 plays a positive regulatory role in drought stress responses in transgenic tobacco plants.

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“…Over-expression of DREB1 by the 35S CaMV promoter increases stress tolerance to drought, high salinity, and freezing in transgenic Arabidopsis plants [31,32]. The ectopic over-expression of Solanum DREB1 confers increased tolerance to high-salt conditions [33]. These results suggest that ZmCIPK21 may play a positive role in salt stress by regulating DREB1B and DREB1C in Arabidopsis .…”

Section: Discussionmentioning

confidence: 99%

ZmCIPK21, A Maize CBL-Interacting Kinase, Enhances Salt Stress Tolerance in Arabidopsis thaliana

Chen

Huang

Zhang

et al. 2014

IJMS

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Salt stress represents an increasing threat to crop growth and yield in saline soil. In this study, we identified a maize calcineurin B-like protein-interacting protein kinase (CIPK), ZmCIPK21, which was primarily localized in the cytoplasm and the nucleus of cells and displayed enhanced expression under salt stress. Over-expression of ZmCIPK21 in wild-type Arabidopsis plants increased their tolerance to salt, as supported by the longer root lengths and improved growth. The downstream stress-response genes, including dehydration-responsive element-binding (DREB) genes were also activated in transgenic plants over-expressing ZmCIPK21. In addition, introduction of the transgenic ZmCIPK21 gene into the Arabidopsis mutant cipk1-2 rescued the salt-sensitive phenotype under high salt stress. Measurement of Na+ and K+ content in transgenic plants showed that over-expression of ZmCIPK21 decreased accumulation of Na+ and allowed retention of relatively high levels of K+, thereby enhancing plant tolerance to salt conditions.

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Ectopic expression of wheat TaCIPK14, encoding a calcineurin B‐like protein‐interacting protein kinase, confers salinity and cold tolerance in tobacco

Cited by 74 publications

References 55 publications

Comparative Phosphoproteomics Reveals an Important Role of MKK2 in Banana (Musa spp.) Cold Signal Network

Comparative Phosphoproteomics Reveals an Important Role of MKK2 in Banana (Musa spp.) Cold Signal Network

A CBL-Interacting Protein Kinase TaCIPK2 Confers Drought Tolerance in Transgenic Tobacco Plants through Regulating the Stomatal Movement

ZmCIPK21, A Maize CBL-Interacting Kinase, Enhances Salt Stress Tolerance in Arabidopsis thaliana

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