ZmCIPK21, A Maize CBL-Interacting Kinase, Enhances Salt Stress Tolerance in Arabidopsis thaliana

Chen, Xunji; Huang, Quansheng; Zhang, Fan; Wang, Bo; Wang, Jianhua; Zheng, Jun

doi:10.3390/ijms150814819

Cited by 38 publications

(26 citation statements)

References 39 publications

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“…Direct ROS level measurement is used as an effective index to evaluate multiple stress tolerance in plants 42 . For example, higher ROS levels were recorded under salt stress in Arabidopsis Atcipk24 mutant while ZmCIPK21 overexpressing lines in maize exhibited lower levels of ROS compared to the control plants 44,45 . In our study, staining with a ROS sensitive fluorescent dye, H 2 DCFDA was used during NaCl treatment to monitor the visible differences in ROS levels in stomatal guard cells.…”

Section: Discussionmentioning

confidence: 99%

A CBL-interacting protein kinase AdCIPK5 confers salt and osmotic stress tolerance in transgenic tobacco

Singh

Shukla

Kirti

2020

Sci Rep

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cBL interacting protein kinases play important roles in adaptation to stress conditions. in the present study, we isolated a CBL-interacting protein kinase homolog (AdCIPK5) from a wild peanut (Arachis diogoi) with similarity to AtCIPK5 of Arabidopsis. Expression analyses in leaves of the wild peanut showed AdCIPK5 induction by exogenous signaling molecules including salicylic acid, abscisic acid and ethylene or abiotic stress factors like salt, PEG and sorbitol. The recombinant AdCIPK5-GFP protein was found to be localized to the nucleus, plasma membrane and cytoplasm. We overexpressed AdCIPK5 in tobacco plants and checked their level of tolerance to biotic and abiotic stresses. While wild type and transgenic plants displayed no significant differences to the treatment with the phytopathogen, Phytophthora parasitica pv nicotianae, the expression of AdCIPK5 increased salt and osmotic tolerance in transgenic plants. Analysis of different physiological parameters revealed that the transgenic plants maintained higher chlorophyll content and catalase activity with lower levels of H 2 o 2 and MDA content during the abiotic stress conditions. AdCIPK5 overexpression also contributed to the maintenance of a higher the K + /na + ratio under salt stress. The enhanced tolerance of transgenic plants was associated with elevated expression of stress-related marker genes; NtERD10C, NtERD10D, NtNCED1, NtSus1, NtCAT and NtSOS1. Taken together, these results indicate that AdCIPK5 is a positive regulator of salt and osmotic stress tolerance. Plants have developed an elaborate network of signaling pathways to counter the challenges posed by various stressful environmental conditions. In general, Ca 2+ serves as an ubiquitous secondary messenger that is reported to be involved in several biological processes including abiotic stress responses, pathogen defense and ion homeostasis adjustment 1. Studies on Ca 2+ dynamics have indicated stimulus-specific elevations in cytosolic Ca 2+ concentration, termed as 'calcium signatures'. To decode each signature, cells retain precise tools and mechanisms that include Ca 2+ sensors and their downstream target proteins 2. The sensor proteins exhibit a Ca 2+ binding site in their helix-loop-helix region 3. These proteins are classified into two categories as sensor responders and sensor relays 4. Sensor responders such as Ca 2+ dependent protein kinases (CDPKs) contain both Ca 2+ binding and kinase activities, while sensor relays like calmodulin (CaM) and calmodulin-like proteins (CML) do not exhibit kinase activity. However, after binding with Ca 2+ , they interact with other protein kinases to regulate their activities 5. Compared to other organisms, the Ca 2+ signaling mechanism in plants is more complex with the acquisition of several specific Ca 2+ sensor proteins. The calcineurin B-like protein (CBL) family is one of these specific sensor proteins. Arabidopsis CBL4/SOS3 is the first CBL protein reported from the plant system 6. Since then, several CBL proteins have been identified from diff...

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Section: Discussionmentioning

confidence: 99%

A CBL-interacting protein kinase AdCIPK5 confers salt and osmotic stress tolerance in transgenic tobacco

Singh

Shukla

Kirti

2020

Sci Rep

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“…4e), suggesting that OST1 may also play crucial roles in the high salt tolerance of Spartina via post-translational regulation of salt stress related genes. CIPKs from many species including rice, maize and wheat have been reported to play important roles in salt tolerance and the overexpression of CIPKs significant improved salt tolerance of transgenic plants (X. Chen et al, 2014; Deng et al, 2013; Xiang, Huang, & Xiong, 2007).…”

Section: Discussionmentioning

confidence: 99%

The full-length transcriptome of Spartina alterniflora reveals the complexity of high salt tolerance in monocotyledonous halophyte

Fraser

Wang

Wei

et al. 2019

Preprint

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18 Spartina alterniflora (Spartina) is the only halophyte in the salt marsh. However, the 19 molecular basis of its high salt tolerance remains elusive. In this study, we used 20 PacBio full-length single molecule long-read sequencing and RNA-seq to elucidate 21 the transcriptome dynamics of high salt tolerance in Spartina by salt-gradient 22 Spartina. Overall, this study sheds light on the high salt tolerance mechanisms of 34 monocotyledonous-halophyte and demonstrates the potential of Spartina genes for 35 engineering salt-tolerant plants. 36 Running title: Spartina transcriptome under salt stress 37 Keywords: Spartina alterniflora; high salt tolerance; ion transporter; protein kinase; 38 regulatory hub genes; alternative splicing; single molecule real-time sequencing 39 40 41 42 43 44 45 46 47 48 alterniflora exhibits a delicate ion exclusion system from physiology aspects to 66 survive hash saline environment and it is a good model plant to study high salt 67 tolerance. However, the molecular basis of salt tolerance in Spartina alterniflora 68 remains unexplored, and the post-transcriptional regulation of high salt tolerance has 69 not been characterized in plants. 70

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“…Na + /K + homeostasis in poplar is regulated by CIPK (CBLinteracting serine/threonine-protein kinase) genes which interact with CBL1 (Calcineurin B-like protein 1) (35). The CIPK genes from wild barley and maize are also known to be implicated in salt tolerance responses (36,37). Furthermore, in tolerant persimmon species such as Diospyros virginiana, salt stress tolerance is believed to be caused by a high a nity potassium transporter HKT-1-like gene in roots (38), which was previously described in other species as key factor in salinity tolerance (39)(40)(41)(42).…”

Section: Introductionmentioning

confidence: 99%

Transcriptomic Analysis Reveals Salt Tolerance Mechanisms Present in Date-Plum Persimmon Rootstock (Diospyros Lotus)

Gil_Muñoz

Delhomme

Quiñones

et al. 2020

Preprint

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Background Drought and salinity are two of the main challenges in agriculture. In many areas, crop production needs solutions to adapt the grown species to the increasing salinity. Research on physiological and molecular responses activated by salinity in plants is needed to elucidate mechanisms of salinity tolerance. Transcriptome profiling (RNA-Seq) is a powerful tool to study the transcriptomic profile of genotypes under stress conditions. In temperate fruit tree species, tree grafting on salinity tolerant rootstocks is a common method to compensate for the cultivar saline sensitivity. Persimmon species have different levels of tolerance to salinity, knowledge of this variability provides the basics for development of salt tolerant rootstocks.Results In this study, we conducted a physiological and transcriptomic profiling of roots and leaves in tolerant and sensitive plants of persimmon rootstock, Diospyros lotus, grown under saline and control conditions. Results from characterization of the physiological responses along with gene expression changes in roots and leaves allowed identifying several salt-tolerance mechanisms related to Ion transport and thermospermine synthesis. Differences were observed in putative H+/ATPases that allow transmembrane ionic transport and Chloride channel protein-like genes. Furthermore, an overexpression of thermospermine synthase found in the roots of tolerant plants may indicate that alterations in root architecture could act as an additional mechanism of response to salt stress. Conclusions Results indicate that D. lotus presents a genetic variability for salt tolerance trait related to the regulation of chloride transport, transmembrane electrochemical potential and thermospermine root synthesis. The study provides knowledge on mechanism of salt stress tolerance in persimmon for further breeding of tolerant persimmon rootstocks.

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ZmCIPK21, A Maize CBL-Interacting Kinase, Enhances Salt Stress Tolerance in Arabidopsis thaliana

Cited by 38 publications

References 39 publications

A CBL-interacting protein kinase AdCIPK5 confers salt and osmotic stress tolerance in transgenic tobacco

A CBL-interacting protein kinase AdCIPK5 confers salt and osmotic stress tolerance in transgenic tobacco

The full-length transcriptome of Spartina alterniflora reveals the complexity of high salt tolerance in monocotyledonous halophyte

Transcriptomic Analysis Reveals Salt Tolerance Mechanisms Present in Date-Plum Persimmon Rootstock (Diospyros Lotus)

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