Ectopic expression of an Eriobotrya japonica APETALA3 ortholog rescues the petal and stamen identities in Arabidopsis ap3-3 mutant

Jing, Danlong; Chen, Weiwei; Shi, Min; Wang, Dan; Xia, Yan; He, Qiang; Dang, Jiangbo; Guo, Qigao; Liang, Guolu

doi:10.1016/j.bbrc.2019.11.177

Cited by 8 publications

(11 citation statements)

References 40 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Following the duplication, the euAP3 lineage acquired a novel C-terminal euAP3 motif instead of paleoAP3 motif and a new role in regulating petal development, while TM6 has preserved the C-terminal paleoAP3 motif [ 12 , 29 ]. In core eudicots, most euAP3-type genes, such as EjAP3 from Eriobotrya japonica [ 30 ], MtNMH7 from Medicago truncatula [ 31 ], PFDEF from Physalis floridana [ 32 ] and GDEF2 from Gerbera hybrid [ 33 ], were expressed only in petal and stamen, and were mainly involved in specifying petal and stamen identities during flower development. All these studies demonstrated that the functions of euAP3-type genes are highly correlated with their expression pattern in core eudicots.…”

Section: Discussionmentioning

confidence: 99%

Isolation and Characterization of APETALA3 Orthologs and Promoters from the Distylous Fagopyrum esculentum

Zeng

Zhang

Wang

et al. 2021

Plants

View full text Add to dashboard Cite

Common buckwheat (Fagopyrum esculentum) produces distylous flowers with undifferentiated petaloid tepals, which makes it obviously different from flowers of model species. In model species Arabidopsis, APETALA3 (AP3) is expressed in petal and stamen and specifies petal and stamen identities during flower development. Combining with our previous studies, we found that small-scale gene duplication (GD) event and alternative splicing (AS) of common buckwheat AP3 orthologs resulted in FaesAP3_1, FaesAP3_2 and FaesAP3_2a. FaesAP3_2 and FaesAP3_2a were mainly expressed in the stamen of thrum and pin flower. Promoters functional analysis suggested that intense GUS staining was observed in the whole stamen in pFaesAP3_2::GUS transgenic Arabidopsis, while intense GUS staining was observed only in the filament of stamen in pFaesAP3_1::GUS transgenic Arabidopsis. These suggested that FaesAP3_1 and FaesAP3_2 had overlapping functions in specifying stamen filament identity and work together to determine normal stamen development. Additionally, FaesAP3_2 and FaesAP3_2a owned the similar ability to rescue stamen development of Arabidopsis ap3-3 mutant, although AS resulted in a frameshift mutation and consequent omission of the complete PI-derived motif and euAP3 motif of FaesAP3_2a. These suggested that the MIK region of AP3-like proteins was crucial for determining stamen identity, while the function of AP3-like proteins in specifying petal identity was gradually obtained after AP3 Orthologs acquiring a novel C-terminal euAP3 motif during the evolution of core eudicots. Our results also provide a clue to understanding the early evolution of the functional specificity of euAP3-type proteins involving in floral organ development in core eudicots, and also suggested that FaesAP3_2 holds the potential application for biotechnical engineering to develop a sterile male line of F. esculentum.

show abstract

Section: Discussionmentioning

confidence: 99%

Isolation and Characterization of APETALA3 Orthologs and Promoters from the Distylous Fagopyrum esculentum

Zeng

Zhang

Wang

et al. 2021

Plants

View full text Add to dashboard Cite

show abstract

“…The tissues of stage I-VIII were collected every 10 days after harvesting fruits until flower initiation. The tissues of stages IX-XIV (S1-S6), which represent different developmental flowers, were collected according to Jing et al [62]. The stems, leaves and flowers used in B, D and F were collected on the same day, which was the full-bloom stage (November 23th).…”

Section: Vector Construction and Quantitative Real-time Pcr (Qrt-pcr)mentioning

confidence: 99%

EjFRI, FRIGIDA (FRI) Ortholog from Eriobotrya japonica, Delays Flowering in Arabidopsis

Chen

Wang

et al. 2020

IJMS

Self Cite

View full text Add to dashboard Cite

In the model species Arabidopsis thaliana, FRIGIDA (FRI) is a key regulator of flowering time and can inhibit flowering without vernalization. However, little information is available on the function in the Rosaceae family. Loquat (Eriobotrya japonica) belongs to the family Rosaceae and is a distinctive species, in which flowering can be induced without vernalization, followed by blooming in late-autumn or winter. To investigate the functional roles of FRI orthologs in this non-vernalization species, we isolated an FRI ortholog, dubbed as EjFRI, from loquat. Analyses of the phylogenetic tree and protein sequence alignment showed that EjFRI is assigned to eurosids I FRI lineage. Expression analysis revealed that the highest expression level of EjFRI was after flower initiation. Meanwhile, EjFRI was widely expressed in different tissues. Subcellular localization of EjFRI was only detected to be in the nucleus. Ectopic expression of EjFRI in wild-type Arabidopsis delayed flowering time. The expression levels of EjFRI in transgenic wild-type Arabidopsis were significantly higher than those of nontransgenic wild-type lines. However, the expression levels of AtFRI showed no significant difference between transgenic and nontransgenic wild-type lines. Furthermore, the upregulated AtFLC expression in the transgenic lines indicated that EjFRI functioned similarly to the AtFRI of the model plant Arabidopsis. Our study provides a foundation to further explore the characterization of EjFRI, and also contributes to illuminating the molecular mechanism about flowering in loquat.

show abstract

“…The field experiments were conducted under local legislation and permissions. According to our previous study, the development stages included vegetative apex (S1), flower bud transition and flower bud differentiation (S2), elongation of the main axis in a panicle (S3), rapid panicle elongation (S4), visible floral buds (S5), elongation of branches in a panicle (S6), white corollas (S7) and full bloom (S8) [20]. Then, these samples were frozen immediately in liquid nitrogen and stored at -80˚C until used.…”

Section: Plant Materialsmentioning

confidence: 99%

“…Morphology of developmental stages was collected and photographed using Canon EOS 6D (Canon Inc., Tokyo, Japan) according to the stages described previously [20]. The buds of loquat at the stage of vegetative apex (VA) and flower bud transition (FBT) were fixed in a FAA solution under vacuum.…”

Section: Morphological Analysis Of the Floral Initiationmentioning

confidence: 99%

Comparative transcriptome analysis of flower bud transition and functional characterization of EjAGL17 involved in regulating floral initiation in loquat

et al. 2020

Self Cite

View full text Add to dashboard Cite

Floral initiation plays a critical role for reproductive success in plants, especially fruit trees. However, little information is known on the mechanism of the initiation in loquat (Eriobotrya japonica Lindl.). Here, we used transcriptomic, expression and functional analysis to investigate the candidate genes in floral initiation in loquat. Comparative transcriptome analysis showed differentially expressed genes (DEGs) were mainly enriched in the metabolic pathways of plant hormone signal transduction. The DEGs were mainly involved in the gibberellin, auxin, cytokinin, abscisic acid, salicylic acid and ethylene signaling pathways. Meanwhile, some transcription factors, including MADS-box (MCM1, AGAMOUS, DEFI-CIENS and SRF), MYB (Myeloblastosis), TCP (TEOSINTE BRANCHED 1, CYCLOIDEA and PCF1), WOX (WUSCHEL-related homeobox) and WRKY (WRKY DNA-binding protein), were significantly differentially expressed. Among these key DEGs, we confirmed that an AGL17 ortholog EjAGL17 was significantly upregulated at the flower bud transition stage. Phylogenetic tree analysis revealed that EjAGL17 was grouped into an AGL17 clade of MADS-box transcription factors. Protein sequence alignment showed that EjAGL17 included a distinctive C-terminal domain. Subcellular localization of EjAGL17 was found only in the nucleus. Expression levels of EjAGL17 reached the highest at the development stage of flower bud transition. Moreover, ectopic expression of EjAGL17 in Arabidopsis significantly exhibited early flowering. Our study provides abundant resources of candidate genes for studying the mechanisms underlying the floral initiation in loquat and other Rosaceae species.

show abstract

Ectopic expression of an Eriobotrya japonica APETALA3 ortholog rescues the petal and stamen identities in Arabidopsis ap3-3 mutant

Cited by 8 publications

References 40 publications

Isolation and Characterization of APETALA3 Orthologs and Promoters from the Distylous Fagopyrum esculentum

Isolation and Characterization of APETALA3 Orthologs and Promoters from the Distylous Fagopyrum esculentum

EjFRI, FRIGIDA (FRI) Ortholog from Eriobotrya japonica, Delays Flowering in Arabidopsis

Comparative transcriptome analysis of flower bud transition and functional characterization of EjAGL17 involved in regulating floral initiation in loquat

Contact Info

Product

Resources

About