Ecology of Thermophilic Fungi in Mushroom Compost, with Emphasis on
            <i>Scytalidium thermophilum</i>
            and Growth Stimulation of
            <i>Agaricus bisporus</i>
            Mycelium

Straatsma, G.; Samson, Robert A.; Olijnsma, T.W.; Camp, Huub J. M. Op den; Gerrits, J.P.G.; Griensven, L. J. L. D. van

doi:10.1128/aem.60.2.454-458.1994

Cited by 102 publications

(39 citation statements)

References 26 publications

(16 reference statements)

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Together these data suggested that the enzyme was a monomeric protein. This value was similar to that reported for b-glucosidases from Humicola grisea [9,10], which is considered a synonym of S. thermophilum [11]. Isoelectric focusing (IEF-PAGE) of the purified b-glucosidase indicated a pI of about 6.5, higher than that of most microbial b-glucosidases, which are acidic proteins with pI values ranging from 3.5 to 5.5 [22].…”

Section: Molecular Propertiessupporting

confidence: 85%

“…Moreover, a few microbial b-glucosidases are known to be 0378 activated by glucose [7,8]. Thermophilic filamentous fungi are good producers of b-glucosidases with high thermal stability [9,10], and among these Scytalidium thermophilum is an efficient consumer of cellulosic materials [11]. This report describes the properties of a glucose-and xylose-activated b-glucosidase from this fungus.…”

Section: Introductionmentioning

confidence: 98%

See 1 more Smart Citation

Î²-Glucosidase activity from the thermophilic fungusScytalidium thermophilumis stimulated by glucose and xylose

Zanoelo

Polizeli

Terenzi

et al. 2004

FEMS Microbiology Letters

124

View full text Add to dashboard Cite

An inducible mycelial beta-glucosidase from Scytalidum thermophilum was characterized. The enzyme exhibited a pI of 6.5, a carbohydrate content of 15%, and an apparent molecular mass of about 40 kDa. Optima of temperature and pH were 60 degrees C and 6.5, respectively. The enzyme was stable up to 1 h at 50 degrees C and exhibited a half-life of 20 min at 55 degrees C. The enzyme hydrolyzed p-nitrophenyl-beta-d-glucopyranoside, p-nitrophenyl-beta-d-xylopyranoside, o-nitrophenyl-beta-d-galactopyranoside, p-nitrophenyl-alpha-arabinopyranoside, cellobiose, laminaribiose and lactose. Kinetic studies indicated that the same enzyme hydrolyzed these substrates. Beta-Glucosidase was activated by glucose or xylose at concentration varying from 50 to 200 mM. The apparent affinity constants (K0.5) for glucose and xylose were 36.69 and 43.24 mM, respectively. The stimulatory effect of glucose and xylose on the S. thermophilum beta-glucosidase is a novel characteristic which distinguish this enzyme from all other beta-glucosidases so far described.

show abstract

Section: Molecular Propertiessupporting

confidence: 85%

Section: Introductionmentioning

confidence: 98%

Î²-Glucosidase activity from the thermophilic fungusScytalidium thermophilumis stimulated by glucose and xylose

Zanoelo

Polizeli

Terenzi

et al. 2004

FEMS Microbiology Letters

124

View full text Add to dashboard Cite

show abstract

“…Scytalidium thermophilum ( Cooney & Emerson ) Austwick , Torula thermophila , Humicola grisea var. thermoidea , and Humicola insolens ( T opt 40 °C, T max 58 °C) have been commonly found in mushroom composts, nesting litter of chickens, soil, horse dung and wood chips, and been regarded as a potential resource for polysaccharide‐degrading enzymes and trehalase . Our previous investigation on the distribution of thermophilic fungi in Tengchong Hotspring National Park in Yunnan, China, revealed that the strains of Thermomyces lanuginosus and Scytalidium thermophilum were found in all the sampling sites and to be very prevalent with the frequencies of occurrence at 34.78 % and 28.26 %, respectively …”

Section: Introductionmentioning

confidence: 99%

Metabolites from Two Dominant Thermophilic Fungal Species Thermomyces lanuginosus and Scytalidium thermophilum

Yang

Zhang

Ding

et al. 2020

Chemistry & Biodiversity

View full text Add to dashboard Cite

Thermomyces lanuginosus and Scytalidium thermophilum are among the most ubiquitous thermophilic fungi in compost and soil. Chemical study on these two prevalent strains collected from Yunnan led to isolation of 23 metabolites, including one new metabolite, therlanubutanolide, and 15 known compounds, isolated from the YGP culture broth of Thermomyces lanuginosus and 7 known compounds isolated from Scytalidium thermophilum, respectively. Therlanubutanolide shared the quite similar features of the same carbon skeleton and saturation as natural hexadecanoic acids. This was the first reported discovery of such a lactone as natural occurring metabolite. All the compounds were reported for the first time from thermophilic fungi. Among them, N-[(2S,3R,4E,8E)-1,3-dihydroxy-9-methyloctadeca-4,8-dien-2-yl]acetamide was for the first time reported to be a naturally occurring metabolite and its NMR data was first provided in this study. A type of PKS-derived metabolites, three 3,4-dihydronaphthalen-1(2H)-ones, which were widely found in plant pathogenic fungi as phytotoxins and reported to have antimicrobial activity, were obtained from both dominant thermophilic fungi. The frequent occurrence of such PKS phytotoxins in these two thermophilic fungi might suggest particular ecological interest.

show abstract

“…The microbial ecology of the substrate is the underlying drive of the composting process and has been the impetus of several studies in the past, mainly for A. bisporus cultivation. Most of these studies used culture-dependent methods to isolate the microbes from compost (Fordyce 1970;Fergus 1964;Fermor et al 1979;Straatsma et al 1989Straatsma et al , 1994.…”

Section: Introductionmentioning

confidence: 99%

Exploring oyster mushroom (Pleurotus ostreatus) substrate preparation by varying phase I composting time: changes in bacterial communities and physicochemical composition of biomass impacting mushroom yields

et al. 2018

View full text Add to dashboard Cite

Aims To investigate characterization of the bacterial community composition and functionality and their impact on substrate biodegradation as well as mushroom yield. Methods and Results Bacterial diversity, composition and functionality were accessed by DNA‐derived analysis for a sugarcane straw‐based substrate composted for either 5, 10 or 15 days. In addition, carbon and nitrogen losses, carbohydrate conversion and mushroom yields were measured for the different treatments. Changes were observed in the bacterial community diversity and composition after the process started, but not during the composting process itself. Following phase I, Acinetobacter sequences were recovered in high numbers, and selected genes associated with nitrogen metabolism and lignocellulose deconstruction were mapped. Substrate physicochemical composition showed elevated carbon and nitrogen losses after 10 and 15 days of phase I with reductions in mushroom yield. Conclusions Acinetobacter species appear to play an important role in substrate degradation processes, and a 5‐day phase I period showed a significant higher mushroom yield compared to composting for either 10 or 15 days. Significance and Impact of the Study This study confers a better understanding of the bacterial community manipulation during the substrate preparation and their influence in substrate selectivity for the Pleurotus ostreatus cultivation.

show abstract

Ecology of Thermophilic Fungi in Mushroom Compost, with Emphasis on Scytalidium thermophilum and Growth Stimulation of Agaricus bisporus Mycelium

Cited by 102 publications

References 26 publications

Î²-Glucosidase activity from the thermophilic fungusScytalidium thermophilumis stimulated by glucose and xylose

Î²-Glucosidase activity from the thermophilic fungusScytalidium thermophilumis stimulated by glucose and xylose

Metabolites from Two Dominant Thermophilic Fungal Species Thermomyces lanuginosus and Scytalidium thermophilum

Exploring oyster mushroom (Pleurotus ostreatus) substrate preparation by varying phase I composting time: changes in bacterial communities and physicochemical composition of biomass impacting mushroom yields

Contact Info

Product

Resources

About