Ecological and Taxonomical Perspective of Yeasts in Indonesia

Sjamsuridzal, Wellyzar; Oetari, Ariyanti; Kanti, Atit; Saraswati, Rasti; Nakashima, Chiharu; Widyastuti, Yantyati; Ando, Katsuhiko

doi:10.5454/mi.4.2.3

Cited by 5 publications

(5 citation statements)

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“…This result is similar to the reports obtained in other parts of Viet Nam [5][6][7][8]. However, this result is opposite to the study by Sjamsuridzal et al [20] in which yeasts were isolated from the leaves in the Cibodas (22 strains/12 leaf samples) and no yeast was found from leaf samples in Cibinong.…”

Section: Yeast Diversity Of Collected Samplessupporting

confidence: 83%

Biodiversity of Yeasts Isolated From Con Dao Island – Ba Ria Vung Tau

Lương¹

2018

JST

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During the study on yeast diversity from natural samples in Viet Nam, we have collected 38 samples of flowers, fruits, leaves, litter and soils in Con Dao Island and isolated 54 yeast strains. They were identified based on morphology observation and sequences of D1/D2 domain of 26S rDNA. The results showed that they belonged to 13 genera, 28 species, of which 15 strains were suspected as 13 new species. Thirty-nine strains were identified as Ascomycetes belonging 7 genera, 11 species, and the fifteen remaining strains belonged to Basidomycetes, 6 genera and 13 species. The results also showed that four Ascomycetous and nine Basidomycetous isolated were suspected to be new species. The results also showed that Candida was the genus with highest number isolates-26 strains, and half of these were Candida rugosa. The most diverse species in this study was found in Cryptococcus genus with 7 species from 8 isolates only, and all of them were suspected to be new species. This study also proved that the samples had differences in yeast diversity and species composition among them. Therefore, Con Dao Island is multi potential to discover and publish new yeast species in Vietnam.

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Section: Yeast Diversity Of Collected Samplessupporting

confidence: 83%

Biodiversity of Yeasts Isolated From Con Dao Island – Ba Ria Vung Tau

Lương¹

2018

JST

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“…In the course of our taxonomic studies of fungi in Indonesia, we pointed out the diversity of entomophagous fungi [10,34], plant parasitic fungi [26], and soilborne fungi [34], based on the modern taxonomic criteria. Likewise, Sjamsuridzal et al [31] reported the yeast population in the flowers of several plants in Indonesia to be rich in those diversity. They found genera of yeasts and yeast-like fungi, such as Anomalomyces, Aureobasidium, Bullera, Candida, Cryptococcus, Metschnikowia, Pseudozyma, Rhodotorula, and Rhodosporidium.…”

Section: G+c Content and Dna Relatednessmentioning

confidence: 95%

“…The D1/D2 domain of nuclear LSU rDNA and the ITS regions of the rDNA unit were sequenced directly from polymerase chain reaction (PCR) products using the primer pairs NL1 and NL4, and ITS4 and ITS5. PCR amplifications were conducted under the condition described in Sjamsuridzal et al [31]. The nucleotide sequences were determined by using a Big Dye Terminator v3.1 Cycle Sequencing Ready Reaction Kit (Applied Biosystems, CA) according to the manufacturer's instructions.…”

Section: Rdna Sequence Analysismentioning

confidence: 99%

New Species of the Genus Metschnikowia Isolated from Flowers in Indonesia, Metschnikowia cibodasensis sp. nov.

Sjamsuridzal

Oetari²,

Nakashima

et al. 2013

J. Microbiol. Biotechnol.

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A novel species, Metschnikowia cibodasensis, is proposed to accommodate eight strains (ID03- 0093(T), ID03-0094, ID03-0095, ID03-0096, ID03-0097, ID03-0098, ID03-0099, and ID03-0109) isolated from flowers of Saurauia pendula, Berberis nepalensis, and Brunfelsia americana in Cibodas Botanical Garden, West Java, Indonesia. The type strain of M. cibodasensis is ID03- 0093(T) (= NBRC 101693(T) =UICC Y-335(T) = BTCC-Y25(T)). The common features of M. cibodasensis are a spherical to ellipsoidopedunculate shaped ascus, which contains one or two needleshaped ascospores, and lyse at maturity. Asci generally develop directly from vegetative cells but sometimes from chlamydospores. The neighbor-joining tree based on the D1/D2 domain of nuclear large subunit (nLSU) ribosomal DNA sequences strongly supports that M. cibodasensis (eight strains) and its closest teleomorphic species, M. reukaufii, are different species by a 100% bootstrap value. The type strain of M. cibodasensis, ID03-0093(T), differed from M. reukaufii NBRC 1679(T) by six nt (five substitutions and one deletion) in their D1/D2 region of nLSU rDNA, and by 18 nt (five deletions, four insertions, and nine substitutions) in their internal transcribed spacer regions of rDNA, respectively. Four strains representative of M. cibodasensis (ID03-0093(T), ID03-0095, ID03-0096, and ID03-0099) showed a mol% G+C content of 44.05 ± 0.25%, whereas that of M. reukaufii NBRC 1679(T) was 41.3%. The low value of DNADNA homology (5-16%) in four strains of M. cibodasensis and M. reukaufii NBRC 1679(T) strongly supported that these strains represent a distinct species.

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“…Khamir Ca. tropicalis juga dilaporkan mampu meningkatkan pertumbuhan tanaman padi (Amprayn et al, 2012 (Sjamsuridzal et al, 2010). Rhodotorula telah digunakan sebagai penghasil karoten, genus ini juga dilaporkan berperan sebagai agens biokontrol patogen tanaman (Patiño-Vera et al, 2005;Fahriani & Wiyono, 2018;Setiawan et al, 2020).…”

Section: Ekstraksi Total Genomik Dna Khamirunclassified

Identifikasi Isolat Khamir Berpotensi sebagai Agens Antagonis dan Uji Produksi Toksin Hemolisin

et al. 2021

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Identifikasi khamir dapat dilakukan secara konvensional maupun molekuler. Identifikasi secara konvensional membutuhkan waktu yang lama dan interpretasi hasilnya seringkali bersifat subyektif. Sementara identifikasi khamir dengan metode molekuler dapat memberikan hasil yang lebih akurat dan cepat. Khamir yang berperan sebagai agens antagonis harus aman terhadap organisme nontarget agar dapat diaplikasikan di lapangan. Penelitian ini bertujuan untuk mengidentifikasi isolat-isolat khamir berpotensi antagonis dengan metode molekuler dan mengetahui kemampuan khamir dalam menghasilkan hemolisin sebagai salah satu indikator potensi resiko terhadap mamalia. Identifikasi dan pengujian kemampuan khamir dalam menghasilkan hemolisin dilakukan pada 15 isolat khamir berpotensi antagonis terhadap patogen antraknosa cabai (Colletotrichum acutatum). Identifikasi khamir dilakukan secara molekuler dengan PCR menggunakan primer ITS1 dan ITS4. Penyediaan khamir menggunakan mediaYeast Malt Extract Broth (YMB) dan Potato Dextrose Agar (PDA). Pengujian kemampuan khamir dalam menghasilkan hemolisin menggunakan media blood agar base (Oxoid CM55) ditambah darah domba 5%. Hasil identifikasi menunjukkan bahwa isolat khamir dapat teramplifikasi dengan primer ITS1 dan ITS4 dengan ukuran fragmen produk antara 500-800 pb. Hasil analisis sekuensing didapatkan 6 spesies khamir yaitu Candida tropicalis, Rhodotorula minuta, Aureobasidium pullulans, Pseudozyma hubeiensis, Pseudozyma aphidis, dan Pseudozyma shanxiensis. Uji kemampuan khamir dalam menghasilkan hemolisin menunjukkan bahwa seluruh khamir yang diuji tidak menghasilkan toksin hemolisin sehingga diduga isolat-isolat tersebut tidak patogenik terhadap manusia.

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Ecological and Taxonomical Perspective of Yeasts in Indonesia

Cited by 5 publications

References 0 publications

Biodiversity of Yeasts Isolated From Con Dao Island – Ba Ria Vung Tau

Biodiversity of Yeasts Isolated From Con Dao Island – Ba Ria Vung Tau

New Species of the Genus Metschnikowia Isolated from Flowers in Indonesia, Metschnikowia cibodasensis sp. nov.

Identifikasi Isolat Khamir Berpotensi sebagai Agens Antagonis dan Uji Produksi Toksin Hemolisin

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