New Species of the Genus Metschnikowia Isolated from Flowers in Indonesia, Metschnikowia cibodasensis sp. nov.

Abstract

A novel species, Metschnikowia cibodasensis, is proposed to accommodate eight strains (ID03- 0093(T), ID03-0094, ID03-0095, ID03-0096, ID03-0097, ID03-0098, ID03-0099, and ID03-0109) isolated from flowers of Saurauia pendula, Berberis nepalensis, and Brunfelsia americana in Cibodas Botanical Garden, West Java, Indonesia. The type strain of M. cibodasensis is ID03- 0093(T) (= NBRC 101693(T) =UICC Y-335(T) = BTCC-Y25(T)). The common features of M. cibodasensis are a spherical to ellipsoidopedunculate shaped ascus, which contains one or two needleshaped ascospores, and lyse at maturity. Asci generally develop directly from vegetative cells but sometimes from chlamydospores. The neighbor-joining tree based on the D1/D2 domain of nuclear large subunit (nLSU) ribosomal DNA sequences strongly supports that M. cibodasensis (eight strains) and its closest teleomorphic species, M. reukaufii, are different species by a 100% bootstrap value. The type strain of M. cibodasensis, ID03-0093(T), differed from M. reukaufii NBRC 1679(T) by six nt (five substitutions and one deletion) in their D1/D2 region of nLSU rDNA, and by 18 nt (five deletions, four insertions, and nine substitutions) in their internal transcribed spacer regions of rDNA, respectively. Four strains representative of M. cibodasensis (ID03-0093(T), ID03-0095, ID03-0096, and ID03-0099) showed a mol% G+C content of 44.05 ± 0.25%, whereas that of M. reukaufii NBRC 1679(T) was 41.3%. The low value of DNADNA homology (5-16%) in four strains of M. cibodasensis and M. reukaufii NBRC 1679(T) strongly supported that these strains represent a distinct species.

Melipona stingless bees and honey microbiota reveal the diversity, composition, and modes of symbionts transmission

“…S6 and Table S1 , Supporting Information ). Metschnikowia is usually encountered in the nectar of flowers (Seijo et al 2011 , Sjamsuridzal et al 2013 ) and in honey (Seijo et al 2011 , Wen et al 2017 ), possibly having a role in its final sugar composition (Garcia-Gonzalez et al 2019 , 2020 ). The presence of Metschnikowia at the proboscis and digestive tract of bumblebees (Brysch-herzberg 2004 ) and in Melipona gut microbiota (Cerqueira et al 2021 ) has also been described.…”

Phylogenies in yeast species descriptions: In defense of neighbor‐joining

“…Whereas some appropriately interpret a high bootstrap value as evidence that the integrity of a clade is well supported by the data (e.g., Kaewwichian et al, 2012; Nguyen et al, 2006), others ignore them altogether in interpreting the data (e.g., Gimenez‐Jurado et al, 2003; Hong et al, 2001; Kachalkin et al, 2021; Kurtzman & Droby, 2001; Liu et al, 2018; Sipiczki, 2014; Xue et al, 2006). More worrisome is the suggestion that the presence of a high bootstrap value at a given node constitutes evidence of a clear separation between clades that are joined at that node (e.g., Molnár & Prillinger, 2005; Sjamsuridzal et al, 2013). This is explained further in the caption of Figure 1.…”