Eco-friendly fluorimetric approaches for the simultaneous estimation of the co-administered ternary mixture: etoposide, moxifloxacin and nalbuphine

Abstract

Antineoplastic drugs, etoposide (ETO), are widely used in leukaemia. A patient with leukaemia has a relative infection with pneumonia treated by fluoroquinolones as moxifloxacin HCL (MOX). Because opioid analgesic as nalbuphine HCL (NAL) does not have a ceiling dose, it is used to manage the distasteful sensory in leukaemia. Consequently, green methods for synchronous spectrofluorimetric quantification of a ternary mixture of ETO, MOX and NAL were developed. The first approach relies simply on the estimation of MOX at 371 nm by conventional synchronous fluorimetric technique (Δ λ of 60 nm). The second approach depends on applying the first derivative synchronous fluorimetric technique (Δ λ of 60 nm) for simultaneous estimation of ETO and NAL at 257 and 273 nm, respectively. A good linear correlation was obtained in the ranges of 0.04–0.40, 0.10–1.00 and 0.50–5.00 µg ml −1 for MOX, ETO and NAL, respectively. Moreover, the proposed approaches were successfully applied for the estimation of the studied drugs in the pharmaceutical dosage forms. Additionally, the synchronous assessment of ETO, MOX and NAL in the spiked human urine was successfully attained by the facile protein precipitation technique. The mean % recoveries in spiked human urine were 99.49, 98.07 and 98.48 for MOX, ETO and NAL, respectively.

Green fluorometric strategy for simultaneous determination of the antihypertensive drug telmisartan (A tentative therapeutic for COVID-19) with Nebivolol in human plasma

“…The sensitivity, simplicity and selectivity of the outstanding spectrofluorometric technique are merits that overweighed the reported spectrophotometric and HPLC methods 32,33 . Constant-wavelength, variety angle, or constant-energy scanning modes of synchronous fluorescence spectroscopy (SFS) are all modes that can be selected for developing spectral simplification and selectivity 34,35 . Constant wavelength monochromator scanning mode, the one that maintains the difference between excitation and emission wavelengths constant (Δλ), is widely utilized to analyze combined pharmaceutical preparations and human plasma.…”