Eco-friendly decoration of graphene oxide with biogenic silver nanoparticles: antibacterial and antibiofilm activity

Faria, Andréia Fonseca de; Moraes, Ana Carolina Mazarin de; Marcato, Priscyla D.; Martinez, Diego Stéfani T.; Durán, Nélson; Filho, A. G. Souza; Brandelli, Adriano; Alves, Oswaldo Luiz

doi:10.1007/s11051-013-2110-7

Cited by 79 publications

(59 citation statements)

References 53 publications

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“…Gram-positive MRSA has a thicker peptidoglycan layer that may provide improved physical protection against the biocidal effects of GO-Ag nanocomposite. 33 Although some studies have attributed antimicrobial activity to pristine GO sheets, 40,41,54,55 our GO sample did not display toxicity to the bacterial strains at the range of concentrations investigated. These contradictory results might be related to the distinctive physicochemical properties of each graphene-based sample, such as surface area, oxidation degree, thickness and lateral dimensions of sheets, carbon precursor source, and purity.…”

Section: International Journal Ofmentioning

confidence: 64%

“…The GO-Ag diffraction peaks observed at 2θ =38.1°, 44.3°, 64.5°, and 77.5° matched the crystalline planes of face-centered cubic silver (Joint Committee on Powder Diffraction Standards [JCPDS] card number 07-0783). 33 Moreover, the peak at 2θ =10.5° that is usually attributed to the stacking of GO sheets disappeared after decoration with the AgNPs. Therefore, the functionalization of the GO surface with the AgNPs might prevent the graphene sheets from restacking.…”

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confidence: 99%

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Graphene oxide-silver nanocomposite as a promising biocidal agent against methicillin-resistant Staphylococcus aureus

Moraes¹,

Lima²,

Faria³

et al. 2015

IJN

114

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Background Methicillin-resistant Staphylococcus aureus (MRSA) has been responsible for serious hospital infections worldwide. Nanomaterials are an alternative to conventional antibiotic compounds, because bacteria are unlikely to develop microbial resistance against nanomaterials. In the past decade, graphene oxide (GO) has emerged as a material that is often used to support and stabilize silver nanoparticles (AgNPs) for the preparation of novel antibacterial nanocomposites. In this work, we report the synthesis of the graphene-oxide silver nanocomposite (GO-Ag) and its antibacterial activity against relevant microorganisms in medicine. Materials and methods GO-Ag nanocomposite was synthesized through the reduction of silver ions (Ag + ) by sodium citrate in an aqueous GO dispersion, and was extensively characterized using ultraviolet-visible absorption spectroscopy, X-ray diffraction, thermogravimetric analysis, X-ray photoelectron spectroscopy, and transmission electron microscopy. The antibacterial activity was evaluated by microdilution assays and time-kill experiments. The morphology of bacterial cells treated with GO-Ag was investigated via transmission electron microscopy. Results AgNPs were well distributed throughout GO sheets, with an average size of 9.4±2.8 nm. The GO-Ag nanocomposite exhibited an excellent antibacterial activity against methicillin-resistant S. aureus , Acinetobacter baumannii , Enterococcus faecalis , and Escherichia coli . All (100%) MRSA cells were inactivated after 4 hours of exposure to GO-Ag sheets. In addition, no toxicity was found for either pristine GO or bare AgNPs within the tested concentration range. Transmission electronic microscopy images offered insights into how GO-Ag nanosheets interacted with bacterial cells. Conclusion Our results indicate that the GO-Ag nanocomposite is a promising antibacterial agent against common nosocomial bacteria, particularly antibiotic-resistant MRSA. Morphological injuries on MRSA cells revealed a likely loss of viability as a result of the direct contact between bacteria and the GO-Ag sheets.

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Section: International Journal Ofmentioning

confidence: 64%

mentioning

confidence: 99%

Graphene oxide-silver nanocomposite as a promising biocidal agent against methicillin-resistant Staphylococcus aureus

Moraes¹,

Lima²,

Faria³

et al. 2015

IJN

114

View full text Add to dashboard Cite

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“…For instance, De Faria et al [51] achieved a completed inhibition of Salmonella typhimurium with 2.0 ppm using graphene oxide and AgNPs hybrid material. Kalpana and Lee [18] synthesized AgNPs from K. pneumoniae grown in a complex simulated microgravity system and found bactericidal effect against S. enterica at range of 50-100 ppm.…”

Section: Antimicrobial Activity Of Phbv-agnpsmentioning

confidence: 99%

Stabilization of antimicrobial silver nanoparticles by a polyhydroxyalkanoate obtained from mixed bacterial culture

Castro-Mayorga

Martínez‐Abad

Fabra

et al. 2014

International Journal of Biological Macromolecules

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“…[1][2][3][4][5] For the AgNPs to be successfully attached, a suitable match between the solid surface and the nanoparticle physicochemical characteristics must occur. By synthesizing AgNPs with the mediation of bacteria and fungi extracts (biogenic AgNPs or bio-AgNPs), the resulting protein-stabilizing capping agent over the nanoparticle contains a variety of chemical groups bonded to the amino acids fragments.…”

Section: Introductionmentioning

confidence: 99%

“…[6][7][8] Essentially, bio-AgNPs are formed from interaction mechanisms ruled by proteins present in the bacterial/ fungal extract or filtrate, which drive the bio-AgNPs' nucleation → growth → colloidal stabilization steps. 2,9,10 At the end of the kinetic process, a protein corona phase on the bio-AgNPs provides a long-term colloidal stability. In contrast, "synthetic" AgNPs are produced from the reduction of Ag + and commonly stabilized by small capping agents of low molecular complexity, such as citrate and sodium dodecyl sulfate (SDS).…”

mentioning

confidence: 99%

Silver Nanocoatings at Large Length Scales: Influence of the AgNPs Morphology and Capping Agents on the Coating Chemical Stability and Antimicrobial Effect

Sousa¹,

Noronha²,

Machado³

et al. 2016

J. Braz. Chem. Soc.

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We assessed at multiple length scales (nanometers to millimeters) the nanocoatings of silver nanoparticles (AgNPs) on model SiO 2 /Si substrates. The coatings from biogenic AgNPs (from yeasts Rhodotorula glutinis and Rhodotorula mucilaginosa) were compared to those formed from "synthetic" AgNPs capped with citrate and sodium dodecyl sulfate (SDS). With computational analysis of large-field (LF) X-ray images of the whole substrates (5 × 5 mm), we were able to assess the coatings homogeneity, relative amount of AgNPs, and their distribution as agglomerates. Surprisingly, by analyzing more than 100,000 elements (nanoparticles and agglomerates) in each sample, it was observed that the mentioned features have little dependence on the AgNPs morphology and capping agents. All silver nanocoatings resisted when immersed in phosphatebuffered saline medium by forming agglomerates of up to 10 μm 2 . However, coatings formed with synthetic AgNPs (capped with citrate and SDS) led to a higher antimicrobial efficiency against Staphylococcus aureus.Keywords: nanobiotechnology, image processing, confocal laser scanning microscopy, scanning electron microscopy, large-field X-ray imaging IntroductionSilver nanoparticles (AgNPs) have many technological applications as films and coatings over solid surfaces such as metals, ceramic, glasses and polymers, to prevent growth of bacteria and fungi. [1][2][3][4][5] For the AgNPs to be successfully attached, a suitable match between the solid surface and the nanoparticle physicochemical characteristics must occur. By synthesizing AgNPs with the mediation of bacteria and fungi extracts (biogenic AgNPs or bio-AgNPs), the resulting protein-stabilizing capping agent over the nanoparticle contains a variety of chemical groups bonded to the amino acids fragments. This latter aspect may lead to a myriad of possible chemical interactions (e.g. electrostatic, hydrophobic, hydrogen bonds and van der Waals) between the nanoparticles and solid surfaces. [6][7][8] Essentially, bio-AgNPs are formed from interaction mechanisms ruled by proteins present in the bacterial/ fungal extract or filtrate, which drive the bio-AgNPs' nucleation → growth → colloidal stabilization steps. 2,9,10 At the end of the kinetic process, a protein corona phase on the bio-AgNPs provides a long-term colloidal stability. In contrast, "synthetic" AgNPs are produced from the reduction of Ag + and commonly stabilized by small capping agents of low molecular complexity, such as citrate and sodium dodecyl sulfate (SDS). 11,12 Several insightful studies in the literature [13][14][15] reported the formation of coatings and films of AgNPs (both biogenic and synthetic) and their dependence on the chemical functionalization of the solid surfaces upon which they are attached. However, the characterization of these coatings are limited to small length scales (up to microns), commonly achieved through imaging techniques such as electron microscopy performed at high magnifications.1,2,4,16-25 A complete understanding of the Silver Nanocoating...

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Eco-friendly decoration of graphene oxide with biogenic silver nanoparticles: antibacterial and antibiofilm activity

Cited by 79 publications

References 53 publications

Graphene oxide-silver nanocomposite as a promising biocidal agent against methicillin-resistant Staphylococcus aureus

Graphene oxide-silver nanocomposite as a promising biocidal agent against methicillin-resistant Staphylococcus aureus

Stabilization of antimicrobial silver nanoparticles by a polyhydroxyalkanoate obtained from mixed bacterial culture

Silver Nanocoatings at Large Length Scales: Influence of the AgNPs Morphology and Capping Agents on the Coating Chemical Stability and Antimicrobial Effect

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