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In this work, novel active edible films having antiviral activity were developed through the solvent casting method. Emulsified edible films were prepared by adding lipids into alginate films in order to improve their water vapour permeability. In addition, two natural extracts rich in phenolic compounds, specifically a green tea extract (GTE) and a grape seed extract (GSE) were used as active agents. Interestingly, incorporating GTE or GSE within the biopolymer matrix, the films exhibited significant antiviral activity against murine norovirus (MNV) and hepatitis A virus (HAV) since 2 log reduction were recorded for the 0.75g extract/g alginate in the film, being GTE more efficient than GSE. Furthermore, the incorporation of phenolic compounds imparted antioxidant capacity to the active films, showing 14.79 ± 0.25 and 3.50± 0.25 mM Trolox g extract in the film-1 for 0.75 GTE and 0.75 GSE films, respectively. It was also found that water barrier efficiency improved upon addition of the highest amount of GTE or GSE (1:0.75 alginate:extract ratio) which was ascribed to molecular interactions between the films´ components, as demonstrated by infrared spectroscopy assays. These results reveal the potential of these active films, mainly the ones containing GTE, to improve food quality and safety.

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