Ecdysteroid Titer, Responsiveness of Prothoracic Gland to Prothoracicotropic Hormone (PTTH), and PTTH Release of the Recessive Trimolter Strain of Bombyx mori in Extra-ecdysed Larvae by JHA and 20E Application

Mitsuoka, Takeshi; Takita, Masami; Kanke, Eiji; Kawasaki, Hideki

doi:10.2108/zsj.18.235

Cited by 6 publications

(15 citation statements)

References 17 publications

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“…Methoprene was applied along the dorsal surface as described in previous studies (Mitsuoka et al, 2001). Larvae were treated with 2 mg methoprene daily during the first 3 days of fourth larval instar.…”

Section: Application Of Methoprenementioning

confidence: 99%

“…B. mori normally has five larval instars with developmental commitment to metamorphosis occurring early in the 5th (final) instar. However, the number of molting in several mutants varies from two to six (Tajima, 1964;Gu et al, 1995;Mitsuoka et al, 2001). Among these mutants, the trimolter mutants undergo precocious metamorphosis at the end of the fourth larval instar (Gu et al, 1995;Mitsuoka et al, 2001).…”

Section: Introductionmentioning

confidence: 99%

“…However, the number of molting in several mutants varies from two to six (Tajima, 1964;Gu et al, 1995;Mitsuoka et al, 2001). Among these mutants, the trimolter mutants undergo precocious metamorphosis at the end of the fourth larval instar (Gu et al, 1995;Mitsuoka et al, 2001). The tetramolter can be induced from recessive trimolter mutants by application of either JHA or 20E (Komori, 1981;Gu et al, 1995;Mitsuoka et al, 2001).…”

Section: Introductionmentioning

confidence: 99%

“…Among these mutants, the trimolter mutants undergo precocious metamorphosis at the end of the fourth larval instar (Gu et al, 1995;Mitsuoka et al, 2001). The tetramolter can be induced from recessive trimolter mutants by application of either JHA or 20E (Komori, 1981;Gu et al, 1995;Mitsuoka et al, 2001). Our previous studies showed that feeding on 20E-induced extra larval ecdysis by maintaining substantial levels of ecdysteroid in the hemolymph and that application of JHA enhanced the PTTH-secreting activity of the brain (Mitsuoka et al, 2001).…”

Section: Introductionmentioning

confidence: 99%

“…The tetramolter can be induced from recessive trimolter mutants by application of either JHA or 20E (Komori, 1981;Gu et al, 1995;Mitsuoka et al, 2001). Our previous studies showed that feeding on 20E-induced extra larval ecdysis by maintaining substantial levels of ecdysteroid in the hemolymph and that application of JHA enhanced the PTTH-secreting activity of the brain (Mitsuoka et al, 2001). However, the molecular basis of these effects remains unclear.…”

Section: Introductionmentioning

confidence: 99%

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20-hydroxyecdysone and juvenile hormone analog prevent precocious metamorphosis in recessive trimolter mutants of Bombyx mori

Wang

Ali

Moriyama

et al. 2012

Insect Biochemistry and Molecular Biology

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Section: Application Of Methoprenementioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 3 more Smart Citations

20-hydroxyecdysone and juvenile hormone analog prevent precocious metamorphosis in recessive trimolter mutants of Bombyx mori

Wang

Ali

Moriyama

et al. 2012

Insect Biochemistry and Molecular Biology

Self Cite

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DECREASED JH BIOSYNTHESIS IS RELATED TO PRECOCIOUS METAMORPHOSIS IN RECESSIVE TRIMOLTER (rt) MUTANTS OF THE SILKWORM, Bombyx mori

Tsai

2012

Arch Insect Biochem Physiol

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In recessive trimolter (rt) mutants of the silkworm, Bombyx mori, that have four larval instars rather than five larval instars of normal B. mori, a decrease after a small increase in the hemolymph ecdysteroid titer during the early stages of the last (fourth) larval instar appeared to be a prerequisite for larvae to undergo precocious metamorphosis. The present study was carried out to investigate the possible mechanism underlying this decrease in the ecdysteroid titer. It was found that juvenile hormone (JH) biosynthetic activity of the corpora allata (CA) increased during the first day of the last larval instar, but its absolute JH biosynthesis activity was relatively lower compared to that of normal fourth-instar larvae in tetramolters. This lowered JH biosynthetic activity appeared to be related to a decrease in prothoracic gland ecdysteroidogenesis during the second day of the last instar, because hydroprene application prevented this decrease in prothoracic gland ecdysteroidogenesis, leading to the induction of a supernumerary larval molt. The in vitro incubation of prothoracic glands with hydroprene showed that hydroprene did not directly exert its action on prothoracicotropic hormone (PTTH) release. Further study showed that the application of hydroprene enhanced the competency of the glands to respond to PTTH. From these results, it was supposed that the lowered JH biosynthesis of the CA during the first day of last instar in rt mutants was related to decreased ecdysteroidogenesis in the prothoracic glands during the second day, thus playing a role in leading to precocious metamorphosis.

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Assessment of toxic and developmental impacts of methoprene on the grey flesh fly Parasarcophaga argyrostoma (Diptera: Sarcophagidae)

2018

Int. J. Biosci.

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Beside its role in human cutaneous wounds and eye myiasis, the grey flesh fly Parasarcophaga argyrostoma (Robineau-Desvoidy) (Diptera: Sarcophagidae) is known as parasitoid of various animals. Objective of the current study was the evaluation of methoprene impacts on survival, development and metamorphosis of this fly.Five doses (10.0, 5.0, 1.0, 0.1 and 0.01µg/larva) were prepared and topically applied onto the early last (3 rd ) instar larvae and prepupae.Thirty replicates (one larva/replicate) of healthy larvae or prepupae were topically treated, individually using Hamilton microapplicator (NHN 737). Similar number of replicates of larvae and

show abstract

Ecdysteroid Titer, Responsiveness of Prothoracic Gland to Prothoracicotropic Hormone (PTTH), and PTTH Release of the Recessive Trimolter Strain of Bombyx mori in Extra-ecdysed Larvae by JHA and 20E Application

Cited by 6 publications

References 17 publications

20-hydroxyecdysone and juvenile hormone analog prevent precocious metamorphosis in recessive trimolter mutants of Bombyx mori

20-hydroxyecdysone and juvenile hormone analog prevent precocious metamorphosis in recessive trimolter mutants of Bombyx mori

DECREASED JH BIOSYNTHESIS IS RELATED TO PRECOCIOUS METAMORPHOSIS IN RECESSIVE TRIMOLTER (rt) MUTANTS OF THE SILKWORM, Bombyx mori

Assessment of toxic and developmental impacts of methoprene on the grey flesh fly Parasarcophaga argyrostoma (Diptera: Sarcophagidae)

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