A male-produced crude aggregation pheromone from Eysarcoris lewisi (Distant) (Heteroptera: Pentatomidae) was collected from volatiles emitted by adult males. It was purified with high performance liquid chromatography using a silica gel column, followed by preparative gas chromatography. The attraction activity of each fraction was field tested using water pan traps. The isolated compound of the aggregation pheromone component was analyzed by GC-MS and NMR, and the chemical structure was estimated to be (Z)-2-methyl-6-(4-methylenebicyclo[3.1.0]hex-1-yl)hept-2-en-1-ol.
Abstract:The efficiency of a pheromone trap was evaluated as an alternative to the "sweeping method" that has been established as a monitoring method of the rice leaf bug, Trigonotylus caelestialium. A sticky trap, a plate with sticky surfaces on both sides, was used and a rubber septum impregnated with 0.01 mg of the synthetic sex pheromone was placed at the center of the upper side of the plate. The sticky traps were installed in paddy fields in Joetsu and Nagaoka, Niigata Prefecture and Toyama, Toyama Prefecture. The most effective trap height was estimated to be near the canopy of rice plants; therefore, the sticky trap was placed by poles at the height of the canopy in the center of a paddy field, and the traps were checked every day throughout the growing season in 2005. Monitoring by means of the sweeping method was also performed in the same fields at approximately five-day intervals. The number of adults captured by sweeping increased from the middle of June to early or mid-July, and then decreased, irrespective of region or rice variety, and the adults increased again at the heading time of each rice variety. The fluctuation pattern of males caught in traps and the number of males captured by sweeping were roughly synchronized in each paddy field. This result suggests that the pheromone trap can be utilized as a monitoring tool for the rice leaf bugs in paddy fields.
Male adults of Eysarcoris lewisi (Distant) reared with a long photoperiod (16L-8D) attracted conspecific adults and nymphs. Males reared with a short photoperiod (8L-16D) showed no attraction. Seasonal fluctuations of adults and nymphs attracted to males reared with a long photoperiod were investigated using water-pan traps on grasslands in Yamagata Prefecture in 2001-2003. The peak attraction of adults from late May to early June may correspond to the emergence of hibernating adults. Another peak of attracted adults from late June to early August was probably the first generation of adults; therefore, it is likely that a trap baited with males reared with a long photoperiod is useful to identifiy the seasonal prevalence of the occurrence of bugs on grasslands.
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