20-hydroxyecdysone and juvenile hormone analog prevent precocious metamorphosis in recessive trimolter mutants of Bombyx mori

Wang, Huabing; Ali, Saheb; Moriyama, Minoru; Iwanaga, Masako; Kawasaki, Hideki

doi:10.1016/j.ibmb.2011.11.002

Cited by 13 publications

(12 citation statements)

References 40 publications

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“…In addition, the results of the present study show that the level of OfKr-h1 mRNA increased in the pupal stage of O. fuscidentalis; this is in conflict with the developmental expression profiles of Kr-h1 in other insects, in which Kr-h1 mRNA levels were found to peak at the prepupal stage and be low in the pupal stage (Kayukawa et al, 2014;Minakuchi et al, 2008;Minakuchi, Tanaka, Miura, & Tanaka, 2011;Wang, Ali, Moriyama, Iwanaga, & Kawasaki, 2012). These results indicate that the developmental expression profiles of Met, DH-PBAN, and Kr-h1 vary in different tissues, depending on the physiological function of Met, DH-PBAN, and Kr-h1 during development.…”

Section: In Vitro Effects Of Diapause Hormone On Ofdhr Ofecr-a Ancontrasting

confidence: 98%

Diapause hormone directly stimulates the prothoracic glands of diapause larvae under juvenile hormone regulation in the bamboo borer, Omphisa fuscidentalis Hampson

Suang

Hiruma

Kaneko

et al. 2019

Arch Insect Biochem Physiol

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Larval diapause in many lepidopteran insects is induced and maintained by high juvenile hormone (JH). In the case of the bamboo borer, Omphisa fuscidentalis, the effect of JH is the opposite: The application of juvenile hormone analog (JHA: S‐methoprene) terminates larval diapause, unlike in other insect species. Here, we analyzed the expression of JH‐receptor Met, DH‐PBAN, and Kr‐h1 in the subesophageal ganglion (SG) from October to April using semi‐quantitative polymerase chain reaction (PCR). The results show that OfMet and OfDH‐PBAN messenger RNA in the SG are mainly expressed during the larval diapause stage, while OfKr‐h1 increases during the pupal stage. Using tissue culture techniques and an enzyme‐linked immunosorbent assay (ELISA), diapause hormone (DH) was found to induce ecdysteroidogenesis in the culture medium of the prothoracic gland (PG) after incubation for 30 min with 25 ng and 50 ng of DH. Thus, DH is a novel stimulator for the PG. We identified a DHR homolog in the bamboo borer and confirmed that it is expressed in the PG. In addition, for in vitro experiments, DH increased the expression levels of OfDHR, OfEcR‐A, and ecdysone‐inducible genes in the PG. These results demonstrate that DH can function as a prothoracicotropic factor, and this function of DH might be through of DHR expressed on PG cells. Consequently, DH is one of the key factors in larval diapause break which is triggered by JH in the bamboo borer, O. fuscidentalis.

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Section: In Vitro Effects Of Diapause Hormone On Ofdhr Ofecr-a Ancontrasting

confidence: 98%

Diapause hormone directly stimulates the prothoracic glands of diapause larvae under juvenile hormone regulation in the bamboo borer, Omphisa fuscidentalis Hampson

Suang

Hiruma

Kaneko

et al. 2019

Arch Insect Biochem Physiol

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“…Previous studies have reported that, in arthropods, the interaction between 20E and juvenile hormones is crucial for metamorphosis (Zhou et al ., ; H.B. Wang et al ., ; Chen et al ., ). Our results also indicated that BR‐C responses due to 20E signalling are essential for miR‐2703 regulation in chitin biosynthesis (Fig.…”

Section: Discussionmentioning

confidence: 99%

“…Several upstream genes including transcription factors, such as E74, E75 and BR-C, were activated by the 20E signalling pathway to regulate downstream genes during moulting (D'Avino et al, 1995;Stilwell et al, 2003;Dubrovskaya et al, 2004;Basso et al, 2006). Previous studies have reported that, in arthropods, the interaction between 20E and juvenile hormones is crucial for metamorphosis (Zhou et al, 1998;H.B. Wang et al, 2012;Chen et al, 2018).…”

Section: Mechanism Of Changes In the Expression Of Nlchs1a And Mir-27mentioning

confidence: 99%

miR‐2703 regulates the chitin biosynthesis pathway by targeting chitin synthase 1a in Nilaparvata lugens

Chen

Pang

2019

Insect Molecular Biology

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The chitin biosynthesis pathway is an important physiology process in arthropods. However, few microRNAs (miRNAs) involved in the regulation of the chitin biosynthesis pathway in insects have been reported until now. In this study, four groups of samples that either upregulated or downregulated the chitin biosynthesis pathway were collected for deep sequencing, and a total of 15 unique mature miRNAs with significantly different expression levels were found, including 11 known miRNAs and four novel miRNAs. Subsequently, we showed that miR‐2703 and its new target gene chitin synthase 1a are important for ecdysone‐induced chitin biosynthesis in Nilaparvata lugens, a serious insect pest of rice. The nymphs showed an obvious moulting defect phenotype, lower survival rate and significantly reduced chitin content after miR‐2703 feeding or injection. Furthermore, we found that the transcription level of miR‐2703 was not repressed by 20‐hydroxyecdysone signalling after Broad‐Complex (BR‐C) double‐stranded RNA (dsRNA) injection compared with the repressed levels after green fluorescent protein dsRNA injection, suggesting that the involvement of miR‐2703 in the 20‐hydroxyecdysone pathway contributes to BR‐C activity. miR‐2703 regulates the chitin biosynthesis pathway by targeting chitin synthase 1a in response to 20‐hydroxyecdysone signalling.

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“…4.3), while exogenous JH prevents the metamorphic transition through the production of supernumerary nymph, larval or pupal instars (Bounhiol, 1938;Fukuda, 1944;Konopova and Jindra, 2007;Sláma, 1965;Tan et al, 2005;Wang et al, 2012;Wigglesworth, 1934Wigglesworth, , 1936Williams, 1961). While ecdysone is thus needed to initiate both moulting and metamorphosis, it is ultimately the presence or absence of JH that determines the outcome of an ecdysone pulse.…”

Section: Jh and Ecdysone Interplaymentioning

confidence: 99%

The Juvenile Hormone Receptor and Molecular Mechanisms of Juvenile Hormone Action

Dubrovsky

Bernardo

2014

Advances in Insect Physiology

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20-hydroxyecdysone and juvenile hormone analog prevent precocious metamorphosis in recessive trimolter mutants of Bombyx mori

Cited by 13 publications

References 40 publications

Diapause hormone directly stimulates the prothoracic glands of diapause larvae under juvenile hormone regulation in the bamboo borer, Omphisa fuscidentalis Hampson

Diapause hormone directly stimulates the prothoracic glands of diapause larvae under juvenile hormone regulation in the bamboo borer, Omphisa fuscidentalis Hampson

miR‐2703 regulates the chitin biosynthesis pathway by targeting chitin synthase 1a in Nilaparvata lugens

The Juvenile Hormone Receptor and Molecular Mechanisms of Juvenile Hormone Action

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