Early Pathogenesis in Porcine Proliferative Enteropathy caused by Lawsonia intracellularis

Boutrup, Torsten Snogdal; Boesen, H. T.; Boye, Mette; Agerholm, Jørgen Steen; Jensen, Tim Kåre

doi:10.1016/j.jcpa.2010.01.006

Cited by 32 publications

(39 citation statements)

References 22 publications

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“…In pigs there is a relationship between histological lesion and immunohistochemical label (Lawson & Gebhart 2000, Kroll et al 2005) and the same seems to happen in mice. Boutrup et al (2010) observed that L. intracellularis could be found within epithelial cells on the top of the villi, indicating infection in mature enterocytes, implying that these cells are also a target for the bacterium. This may be one possible explanation for the presence of antigens at the apex of the villi at 7 dpi in small intestines of all mice strains tested.…”

Section: Discussionmentioning

confidence: 94%

Infection of sparrows (Passer domesticus) and different mice strains with Lawsonia intracellularis

et al. 2013

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The susceptibility of sparrows (Passer domesticus) and strains of mice (Swiss, BALB/c, C-57 and DB-A) to Lawsonia intracellularis infection was studied. Thirty-two sparrows were inoculated with pure culture of L. intracellularis and eleven received sham inoculum. Feces were collected on -1, 7, 14 and 21 days post infection (dpi) for detection of L. intracellularis by PCR. After 21 days, all sparrows were euthanized and the tissues processed for histology and immunohistochemistry (IHC). One hundred sixty mice of four different strains (n=40, per strain) were used. For each mouse strain, 16 animals received mucosa homogenate from a pig infected with L. intracellularis, 16 received pure culture of L. intracellularis and eight animals received sham inoculum. Two control and four inoculated mice from each group were euthanized on 7, 14, 21 and 28 dpi. Sections of intestine were collected for histologic analysis and IHC and pooled feces were collected for L. intracellularis PCR. None of the sparrows had any histologic lesions characteristic of proliferative enteropathy or antigen labeling by IHC. All sparrow fecal samples were negative by PCR. All mice strains studied had histopathological lesions typical of PE and IHC labeling consistent with L. intracellularis infection, especially those animals inoculated with pure culture. The most severe lesions were observed in DB-A and Swiss mice. Fecal shedding was detected in all mice strains, with peak at 14 dpi. We conclude that sparrows do not seem to be relevant in the epidemiology of L. intracellularis. The results showed variations in the lesions among the four mice strains used. C-57 e DB-A) à infecção por L. intracellularis foi testada. Trinta e dois pardais foram inoculados com cultura pura de L. intracellularis e onze receberam placebo. As fezes foram coletadas nos dias -1, 7, 14 e 21 após a infecção (dpi) para a detecção de Lawsonia intracellularis por PCR. Após 21 dias, todos os pardais foram eutanasiados e os tecidos processados para a realização da histologia e imuno-histoquímica (IHQ

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Section: Discussionmentioning

confidence: 94%

Infection of sparrows (Passer domesticus) and different mice strains with Lawsonia intracellularis

et al. 2013

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“…Esta investigación estandarizó una técnica de pcr anidada para la amplificación de la porción de 270 pb del gen 16s arnr de L. intracellularis, agente causal de la enteritis proliferativa porcina y responsable de importantes pérdidas económicas en el sector porcícola [8]- [10]. La técnica permitió confirmar la presencia de L. intracellularis en las explotaciones del área metropolitana de Bucaramanga.…”

Section: Discussionunclassified

Estandarización de una PCR anidada para la identificación de Lawsonia intracellularis en porcinos

et al. 2014

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Resumen. Introducción: se estandarizó una técnica de Reacción en Cadena de la Polimerasa pcr anidada para el diagnóstico de la enteropatía proliferativa porcina causada por la bacteria Lawsonia intracellularis. Métodos: la técnica se basó en la amplificación de una región de 270 pb del gen 16s arnr de Lawsonia intracellularis; para tal fin, se extrajo el adn de la bacteria mediante el kit Purelink Genomic, Invitrogen ® , a partir de 100 muestras de íleon de porcinos provenientes del área metropolitana de Bucaramanga (Santander). Los adn fueron cuantificados por fluorometría y ajustados a una concentración de 20 ng/µL. Se llevó a cabo una primera amplificación de la porción de 319 pb del gen 16s arn con los iniciadores externos: 5'-tat ggc tgt caa aca ctc cg-3' y 5'-tga agg tat tgg tat tct cc-3' . El producto de esta primera amplificación se usó de molde para una segunda reacción, en la que se amplificó una porción interna de 270 pb del gen 16s arn de L. intracellularis, empleando los iniciadores internos específicos: 5'-tta cag gtg aag tta ttg gg-3' y 5'-ctt tct cat gtc cca taa gc-3' . Las condiciones óptimas para las pcr tanto con los iniciadores internos como con los externos incluyeron: 2,5 µm de mgcl 2 ; 0,15 µm de cada dntp; 1x de Buffer de reacción; 0,8 µm de cada iniciador; y 2,5 U de Taq polimerasa. Como control positivo tanto en la extracción de adn como en las pcr, se empleó adn bacteriano extraído de la vacuna Enterisol ® (Boheringer Ingelheim ® ). Resultados: las temperaturas óptimas de anillamiento para la pcr simple y anidada fueron de 50 y 55°C, respectivamente. El límite de detección de la técnica fue de 5 fg. Conclusiones: la técnica no evidenció reacción cruzada con Esquerichia coli, ni con Salmonella typhimurium, bacterias con cuadros clínicos semejantes.Palabras clave: cerdos, diagnóstico molecular, ileítis. pcr Standardization to Identify Lawsonia intracellularis in PigsAbstract. Introduction: the nested Polymerase Chain Reaction (pcr) technique has been standardized for the diagnosis of porcine proliferative enteropathy caused by Lawsonia intracellularis. Methods: such technique was based upon amplifying a region of 270 pb of the 16s arnr gene of the Lawsonia intracellularis. For such purposes, a dna sample was taken from the bacteria with a Purelink Genomic kit, Invitrogen ® , from 100 samples of porcine ileum of the metropolitan area of Bucaramanga (Santander). The dna samples were quantified with fluorometry and adjusted to 20 ng/µL concentration. The first amplification of the 319 pb part of the 16s gene was completed with the external initiators: 5'-tat ggc tgt caa aca ctc cg-3' and 5'-tga agg tat tgg tat tct cc-3' . The result of this first amplification was used as a pattern for the second reaction, where the internal part of 270 pb of the 16s arn gene of L. intracellularis was amplified using the specific internal initiators: 5'-tta cag gtg aag tta ttg gg-3' and 5'-ctt tct cat gtc cca taa gc-3' . The optimal conditions for the pcr for both internal and external initiators included 2...

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“…These cells replace mature epithelial cells normally lining the small intestinal villi, resulting in malabsorptive diarrhoea and subsequent hypoproteinaemia. It has also been proposed that hypoproteinaemia noted in some affected species can result from the proliferation of the secretory crypt epithelium or occur secondarily to microbial invasion (Cunningham 1997, Cooper and Gebhart 1998, Boutrop and others 2010). …”

Section: Pathogenesismentioning

confidence: 99%

Management of equine proliferative enteropathy

Slovis¹

2016

In Practice

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Equine proliferative enteropathy is caused by Lawsonia intracellularis and mainly affects foals that are between two and 13 months of age. The epidemiology is not fully understood, but it is believed that transmission occurs by ingestion of faecal material from wild or domestic animals. This article describes the clinical signs and diagnosis of equine proliferative enteropathy and how to monitor a herd, together with possible treatment and prevention protocols.

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Early Pathogenesis in Porcine Proliferative Enteropathy caused by Lawsonia intracellularis

Cited by 32 publications

References 22 publications

Infection of sparrows (Passer domesticus) and different mice strains with Lawsonia intracellularis

Infection of sparrows (Passer domesticus) and different mice strains with Lawsonia intracellularis

Estandarización de una PCR anidada para la identificación de Lawsonia intracellularis en porcinos

Management of equine proliferative enteropathy

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