Early identification of hybrids of Indian major carp, <i>Labeo rohita</i>
 and <i>Catla catla</i>
 using nuclear marker-based multiplex PCR

Mohanty, Mausumee; Jayasankar, P.; Sahoo, Lakshman; Das, Paramananda; Das, Basanta Kumar; Verma, Deepti; Routray, P.

doi:10.1111/jai.13111

Cited by 4 publications

(2 citation statements)

References 12 publications

(12 reference statements)

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“…This technology would really be of great use for those producers and buyers for screening of quality seeds and moreover it would be a stepping stone for seed certification programmes for both government and private hatcheries. In a survey conducted by the present author and team in West Bengal, Bihar, Odisha and Gujarat, it has been found that on an average 22.8% of the total hatchery produced seeds are hybrids (Mohanty et al, 2016).…”

Section: Technology Interventions For Aquaculture Developmentmentioning

confidence: 79%

Present status of freshwater aquaculture in India - A review

Jayasankar¹

2018

Indian J. Fish.

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Aquaculture is a rapidly growing fisheries sector in India with an annual growth rate of over 7%. Freshwater aquaculture contributes over 95% of the total annual aquaculture production of 5.77 million t. Technologies of induced carp breeding and polyculture of the three Indian major carps (Catla catla, Labeo rohita and Cirrhinus mrigala) as well as ‘composite carp culture’ with the addition of three exotic carps (Hypophthalmichthys molitrix, Ctenopharyngodon idella and Cyprinus carpio) in ponds and tanks brought about perceptible upward shift in freshwater aquaculture production. Of late, the sector has been witnessing diversification with the inclusion of medium and minor carps, catfishes and murrels. Andhra Pradesh and West Bengal are the top producers of freshwater fish through aquaculture. System diversification has resulted in optimum productivity of 3-6 t ha-1 yr-1. Public and private carp hatcheries produce around 40 billion fry. Fish requirement by 2020 is expected to touch 15 million t, of which around 9 million t would need to come from freshwater aquaculture. Input-related, social and environmental constraints of aquaculture in India need to be tackled through horizontal and vertical expansion, technology as well as climate change mitigation and adaptation, for sustainable development.

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Section: Technology Interventions For Aquaculture Developmentmentioning

confidence: 79%

Present status of freshwater aquaculture in India - A review

Jayasankar¹

2018

Indian J. Fish.

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“…A total of 685 samples of different life stages (spawn and fry) from different hatcheries of four Indian states, Odisha, Gujarat, Bihar, and West Bengal (Table 1), were collected and tested using the species-specific designed primers with the same thermal cycler condition [60].…”

Section: Validation Of Primers By Screening Unknown Samples From Hatc...mentioning

confidence: 99%

Hybridization in Carps and Early Detection of Carp Hybrids Using PCR-Based Kit

Jayasankar¹

2020

Emerging Technologies, Environment and Research for Sustainable Aquaculture

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Hybridization is the mating of genetically differentiated individuals or groups and may involve crosses within a species or between separate species. Hybridization can be natural or human-mediated. Reproductive barriers prevent excessive introgression in the former, whereas more often introgression and genetic pollution happen in the latter. Hybridization is more widespread among members of Cyprinidae than any other groups of freshwater fishes. In many carp hatcheries in India, breeders of catla (Catla catla) and rohu (Labeo rohita) are kept in the same tank for breeding, resulting in production of hybrid seeds. Fish hybrids can pose a serious threat to the aquatic environment biodiversity. Consequently, genetic monitoring of organisms is entailed to unambiguously identify parental species and their hybrids. Adopting a multiplex PCR using β-actin gene primers, a kit has been developed to distinguish between the hybrids from their parental species. Agarose electrophoresis revealed one band of about 100 bp in size specific for rohu, another at 300 bp specific for catla, and both bands in hybrid. The kit was tested successfully with the samples collected from many hatcheries located in four Indian states. The rohu-catla early hybrid identification PCR kit could serve as a stepping stone for carp seed certification and hatchery accreditation.

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