Early differentiation between caries and tooth demineralization using laser‐induced autofluorescence spectroscopy

Borisova, Ekaterina; Uzunov, Ts.; Avramov, L.

doi:10.1002/lsm.20009

Cited by 23 publications

(22 citation statements)

References 21 publications

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“…Besides, some bacteria, causing, e.g., the inflammation diseases of skin [239] and gums [240], inhabiting the caries tooth lesions [241], can accumulate considerable amounts of porphyrins. Combining the measured autofluorescence spectra with those of reflection and absorption, OCT and other optical methods allows the diagnostics of caries and tooth tissue demineralisation [241][242][243][244][245], skin cancer [246,247], and pre-cancer changes of cervical tissue [248], mammary gland [249], and other diseases.…”

Section: Fluorescence Imagingmentioning

confidence: 99%

Optical clearing of biological tissues: prospects of application in medical diagnostics and phototherapy

Genina

Bashkatov

Sinichkin

et al. 2015

JBPE

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Abstract.A review of specific features and methods of optical clearing and related interaction of light with tissues is presented. Physical and molecular mechanisms of immersion, compression, and photodynamic/photothermal optical clearing of some fibrous and cellular tissues are discussed. The possibility of efficient control of the tissue optical properties, particularly, the reduction of light scattering in tissues is demonstrated, which facilitates the increased efficiency of various optical visualisation methods (optical biopsy) used in medical purposes. © 2015 Samara State Aerospace University (SSAU).Keywords: tissue, optical clearing, optical diagnostics, imaging. 1, 404-413 (1997). 5. C. L. Smithpeter, A. K. Dunn, A. J. Welch, and R. Richards-Kortum, "Penetration depth limits of in vivo confocal reflectance imaging," Appl. Opt. 37, 2749Opt. 37, -2754Opt. 37, (1998. 6. V. V. Tuchin, L. V. Wang, and D. A. Zimnyakov, Optical Polarization in Biomedical Applications, SpringerVerlag, New York, NY, USA (2006). 7. R. Drezek, A. Dunn, and R. Richards-Kortum, "Light scattering from cells: finite-difference time-domain simulations and goniometric measurements," Appl. Opt. 38(16), 3651-3661 (1999). 8. K. Sokolov, R. Drezek, K. Gossagee, and R. Richards-Kortum, "Reflectance spectroscopy with polarized light:is it sensitive to cellular and nuclear morphology," Opt. Express 5, 302-317 (1999). 9. D. W. Leonard, and K. M. Meek, "Refractive indices of the collagen fibrils and extrafibrillar material of the corneal stroma," Biophysical J. 72, 1382-1387 (1997). 10. A. G. Borovoi, E. I. Naats, and U. G. Oppel, "Scattering of light by a red blood cell," J. Biomed. Opt. 3, 364-372 (1998). 11. A. N. Yaroslavsky, A. V. Priezzhev, J. Rodriguez, I. V. Yaroslavsky, and H. Battarbee, "Optics of blood,"Chap. 2 in Handbook of Optical Biomedical Diagnostics, V. V. Tuchin, Ed., pp. 169-216, PM107 SPIE Press, Bellingham, WA, USA (2002). 12. G. Mazarevica, T. Freivalds, and A. Jurka, "Properties of erythrocyte light refraction in diabetic patients," J.Biomed. Opt. 7, 244-247 (2002). 13. M. Friebel, and M. Meinke, "Model function to calculate the refractive index of native hemoglobin in the wavelength range of 250-1100 nm dependent on concentration," Appl. Opt. 45(12), 2838-2842 (2006). Appl. Opt. 35(19), 3413-3420 (1996). 34. D. Zhu, J. Wang, Z. Zhi, X. Wen, and Q. Luo, "Imaging dermal blood flow through the intact rat skin with an optical clearing method," J. Biomed. Opt. 15, 026008 (2010 241. K. König, G. Flemming, and R. Hibst, "Laser-induced autofluorescence spectroscopy of dental caries lesion," Cell. Mol. Biol. 44, 1293-1300. 242. E. G. Borisova, T. T. Uzunov, and L. A. Avramov, "Early differentiation between caries and tooth demineralization using laser-induced autofluorescence spectroscopy," Lasers Surg. Med. 34, 249-253 (2004 -20(12), 1512-1516 (1984). 245. K. Konig, H. Schneckenburger, and R. Hibst, "Time-gated in vivo autofluorescence imaging of dental caries,"Cell. Mol. Biol. 45, 233-239 (1999). 246. E. Borisova, P. Tr...

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Section: Fluorescence Imagingmentioning

confidence: 99%

Optical clearing of biological tissues: prospects of application in medical diagnostics and phototherapy

Genina

Bashkatov

Sinichkin

et al. 2015

JBPE

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“…The values of spectroscopic parameters were obtained by analysis of Raman scattering spectra. In comparison with commercially available spectroscopic methods, such as laser‐induced spectroscopy) and quantitative light‐induced fluorescence, we proposed to base the diagnosis on several independent parameters determined by simultaneous analysis of three different physical phenomena – Raman (nonelastic) scattering, Rayleigh (elastic) scattering and fluorescence emission. Such an approach could allow unambiguous determination of caries occurrence in the enamel of the tooth, even at an early stage before detection of visual changes.…”

Section: Introductionmentioning

confidence: 99%

Raman spectroscopy as a tool of early dental caries detection–new insights

Buchwald

Okulus

Szybowicz

2017

J Raman Spectroscopy

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The aim of this study was to investigate the effectiveness of Raman spectroscopy in determination of changes in composition and structure of human tooth enamel with caries by use of new parameters such as the Raman band position, the full width at half maximum of Raman band and line shape of Raman band. The values of spectroscopic parameters were obtained by analysis of Raman scattering spectra. We proposed to base the diagnosis on several parameters determined by simultaneous analysis of Raman scattering, Rayleigh scattering and fluorescence emission. We verified the correlations between the values of the few selected parameters and the severity of dental caries. Raman band spectral parameters, such as position and line shape, can be useful to indicate the enamel caries changes. Moreover, it is possible to detect early caries by analysis of background in the Raman scattering spectrum related to the Rayleigh scattering and fluorescence emission. We demonstrated that a few independent parameters obtained by simultaneous analysis of three different physical phenomena could allow unambiguous determination of the early caries occurrence in the enamel. The published results give new insights into early caries detection and demonstrate the new possibilities of Raman spectroscopy as a diagnostic tool to recognize the caries disease. Copyright © 2017 John Wiley & Sons, Ltd.

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“…The difference in fluorescence intensities between sound and demineralized teeth samples can be explained by the light scattering properties of enamel which gets destroyed after initial caries, causing a reduction in the intensity as stated by Borisova et al 18,19 . Normal enamel is made up of enamel rods which have a prismatic structure and possess wave guide properties 20 .…”

Section: Discussionmentioning

confidence: 92%

Does ozone enhance the remineralizing potential of nanohydroxyapatite on artificially demineralized enamel? A laser induced fluorescence study

Srinivasan

Prabhu

Chandra

et al. 2014

Lasers in Dentistry XX

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The present era of minimal invasive dentistry emphasizes the early detection and remineralization of initial enamel caries. Ozone has been shown to reverse the initial demineralization before the integrity of the enamel surface is lost. Nano-hydroxyapatite is a proven remineralizing agent for early enamel caries. In the present study, the effect of ozone in enhancing the remineralizing potential of nano-hydroxyapatite on artificially demineralized enamel was investigated using laser induced fluorescence. Thirty five sound human premolars were collected from healthy subjects undergoing orthodontic treatment. Fluorescence was recorded by exciting the mesial surfaces using 325 nm He-Cd laser with 2 mW power. Tooth specimens were subjected to demineralization to create initial enamel caries. Following which the specimens were divided into three groups, i.e ozone (ozonated water for 2 min), without ozone and artificial saliva. Remineralization regimen was followed for 3 weeks. The fluorescence spectra of the specimens were recorded from all the three experimental groups at baseline, after demineralization and remineralization. The average spectrum for each experimental group was used for statistical analysis. Fluorescence intensities of Ozone treated specimens following remineralization were higher than that of artificial saliva, and this difference was found to be statistically significant (P<0.0001). In a nutshell, ozone enhanced the remineralizing potential of nanohydroxyapatite, and laser induced fluorescence was found to be effective in assessing the surface mineral changes in enamel. Ozone can be considered an effective agent in reversing the initial enamel caries there by preventing the tooth from entering into the repetitive restorative cycle.

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Early differentiation between caries and tooth demineralization using laser‐induced autofluorescence spectroscopy

Abstract: A differentiation between initial tooth demineralization and early stages of caries could be made by the laser-induced fluorescence spectroscopy method.

Cited by 23 publications

References 21 publications

Optical clearing of biological tissues: prospects of application in medical diagnostics and phototherapy

Optical clearing of biological tissues: prospects of application in medical diagnostics and phototherapy

Raman spectroscopy as a tool of early dental caries detection–new insights

Does ozone enhance the remineralizing potential of nanohydroxyapatite on artificially demineralized enamel? A laser induced fluorescence study

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