Early detection of banana bunchy top virus in india using polymerase chain reaction

Manickam, K.; Doraiswamy, Sabitha; Ganapathy, T.; Rabindran, R.

doi:10.1556/aphyt.37.2002.1-3.2

Cited by 7 publications

(3 citation statements)

References 14 publications

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“…esculenta , and Alpinia zerumbet , further studies have failed to confirm that these species are suitable hosts for other BBTV strains ( Hu et al. 1996 ; Geering and Thomas 1997 ; Manickam et al. 2002 ).…”

Section: Resultsmentioning

confidence: 99%

The global distribution ofBanana bunchy top virusreveals little evidence for frequent recent, human-mediated long distance dispersal events

et al. 2015

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Banana bunchy top virus (BBTV; family Nanoviridae, genus Babuvirus) is a multi-component single-stranded DNA virus, which infects banana plants in many regions of the world, often resulting in large-scale crop losses. We analyzed 171 banana leaf samples from fourteen countries and recovered, cloned, and sequenced 855 complete BBTV components including ninety-four full genomes. Importantly, full genomes were determined from eight countries, where previously no full genomes were available (Samoa, Burundi, Republic of Congo, Democratic Republic of Congo, Egypt, Indonesia, the Philippines, and the USA [HI]). Accounting for recombination and genome component reassortment, we examined the geographic structuring of global BBTV populations to reveal that BBTV likely originated in Southeast Asia, that the current global hotspots of BBTV diversity are Southeast Asia/Far East and India, and that BBTV populations circulating elsewhere in the world have all potentially originated from infrequent introductions. Most importantly, we find that rather than the current global BBTV distribution being due to increases in human-mediated movements of bananas over the past few decades, it is more consistent with a pattern of infrequent introductions of the virus to different parts of the world over the past 1,000 years.

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Section: Resultsmentioning

confidence: 99%

The global distribution ofBanana bunchy top virusreveals little evidence for frequent recent, human-mediated long distance dispersal events

et al. 2015

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“…Based on sequence analysis of the six components, the Lucknow isolate has been identified as a member of South Pacific group of BBTV which further strengthen by the earlier findings of Karan et al [9,10,30] that Indian isolates of BBTV belong to the South Pacific group rather than the Asian group. However, a report on serological characterization of BBTV from south India by immune-sorbent electron microscopy (ISEM) studies is also available in the literature where monoclonal antibodies specific for an Australian isolate capsid protein reacted strongly with the respective BBTV isolate suggesting its close relation to South Pacific group [31].…”

Section: Discussionmentioning

confidence: 99%

Molecular characterization of an Indian isolate of Banana bunchy top virus based on six genomic DNA components

2009

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Banana bunchy top virus (BBTV) is a single-stranded circular DNA virus of the genus Babuvirus, belonging to family Nanoviridae. The six genomic DNA components of Indian (Lucknow) isolate of BBTV were amplified by polymerase chain reaction (PCR) with specific primers using total DNA extracted from banana tissues showing typical symptoms of banana bunchy top disease (BBTD). The resulting ~1.1 Kb amplicons were cloned and sequenced. Analysis of sequence data revealed the presence of six full-length components of BBTV: DNA-R (1111 bp), DNA-U3 (1060 bp), DNA-S (1075 bp), DNA-M (1048 bp), DNA-C (1018 bp), and DNA-N (1096 bp). Comparisons of sequence data of the six DNA components of the BBTV Lucknow isolate revealed highest identities with sequences of other BBTV isolates from the South Pacific group: [DNA-R (98%), DNA-U3 (93%), DNA-S (100%), DNA-M (98%), DNA-C (97%), and DNA-N (99%)]. A phylogenetic analysis revealed a close relationship of the Lucknow isolate with BBTV isolates of South Pacific group rather than those of the Asian group. Based on these analyses the virus has been classified as BBTV Lucknow, a new member of South Pacific group.

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“…Selvarajan et al (2007) also developed a PCR-based detection method for Indian isolates of BBTV. PCR has been used to detect BBTV from viruliferous aphids (Manickam et al, 2002;Selvarajan et al, 2006b). BBrMV was detected by RT-PCR in total nucleic acid extracts from infected plants, using specific or degenerate potyvirus group primers (Bateson and Dale, 1995;Thomas et al, 1997).…”

Section: Polymerase Chain Reaction (Pcr)mentioning

confidence: 99%

Virus-Indexing Technology for Production of Quality Banana Planting Material: A Boon to the Tissue-Culture Industry and Banana Growers in India

Selvarajan¹,

Balasubramanian²,

Sheeba³

et al. 2011

Acta Hortic.

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Banana and plantain (Musa spp.) are the most important fruit crop in India and play a major role in the livelihood of millions of resource-poor small farmers. Use of quality planting material is very important for increasing productivity. Though conventional suckers are still the primary planting material, use of tissueculture plants has increased because of their advantages, like more uniform bunches with even maturity and increased yield. However, banana viral pathogens, which are economically important in India, can be inadvertently spread through tissue-culture plants. In order to control the spread of the viruses, virus-indexing techniques were developed at the National Research Centre for Banana, Tiruchirapalli for early detection in mother plants and tissue-culture plants used for mass propagation. Polymerase chain reaction (PCR), reverse transcriptase PCR (RT-PCR), nonradioactive probe-based nucleic acid spot hybridisation (NASH) and enzyme-linked immune-sorbent assay (ELISA) based techniques were developed and validated for routine testing. PCR and NASH tests are being done for detection of Banana bunchy top virus (BBTV) and Banana streak Mysore virus (BSMysV). RT-PCR and ELISA tests are being done for the detection of Banana bract mosaic virus (BBrMV) and Cucumber mosaic virus (CMV). Based on the work done at the centre, the Department of Biotechnology (DBT), Government of India, has accredited the Molecular Virology lab for testing for banana viruses in the country. In total, 15,850 tissue-culture and mother-plant samples were tested against viruses. The percentage of positive plants for BBTV, BSMysV, CMV and BBrMV were 3.83, 2.85, 17.3 and 0.95% respectively. Indexing was done mostly for the cultivars 'Grande Naine' (AAA, Cavendish subgroup), 'Robusta' (AAA, Cavendish subgroup) and 'Hill Banana' (syn. 'Virupakshi', AAB, Pome subgroup). Around 25 tissue-culture banana commercial laboratories are undertaking the services of virus testing. Our laboratory has also developed polyclonal antisera for CMV and BBrMV through recombinant DNA technology, which is currently being validated for ELISA-based testing.

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Early detection of banana bunchy top virus in india using polymerase chain reaction

Cited by 7 publications

References 14 publications

The global distribution ofBanana bunchy top virusreveals little evidence for frequent recent, human-mediated long distance dispersal events

The global distribution ofBanana bunchy top virusreveals little evidence for frequent recent, human-mediated long distance dispersal events

Molecular characterization of an Indian isolate of Banana bunchy top virus based on six genomic DNA components

Virus-Indexing Technology for Production of Quality Banana Planting Material: A Boon to the Tissue-Culture Industry and Banana Growers in India

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