Early Changes in Energy Metabolism in Rats Exposed to an Acute Level of Deoxycholate and Fed a Nigerian-Like Diet

Abstract

Early alterations in cellular energy metabolism, reductive biosynthesis and enzymes related to cell proliferation were studied in 40 Wistar albino rats exposed to an acute level of deoxycholate (DOC), and fed different diets. The animals were divided into four equal groups and fed ad libitum either a normal diet (ND), a high-carbohydrate high-fibre (HCF) diet, or a high-protein high-fat (HPF) diet. Three times weekly intrarectal injection of 40 mg/0.2 ml DOC was given to three groups of the rats for 9 weeks. The specific activities of phosphofructokinase, pyruvate kinase, lactate dehydrogenase, glucose 6-phosphate dehydrogenase and 6-phosphogluconate dehydrogenase were all significantly (p < 0.05) increased in the DOC-treated animals compared with the physiological saline-treated control. Reductive biosynthetic enzyme activities (malic enzyme, isocitrate dehydrogenase-NADP⁺-dependent, and ATP-citrate lyase) were reduced in the DOC-treated animals compared with the control. Feeding rats with the HCF diet significantly (p < 0.05) lowered the specific activities of the enzymes of glycolysis, of the pentose phosphate pathway (oxidative) and hyaluronidase and proteinase compared with those of the HPF-fed rats. These results show an altered enzymic profile in rats fed an HCF and an HPF diet compared with rats fed the ND and suggests a protective role of the HCF diet against the development of colon cancer.

ATP citrate lyase (ACLY) inhibitors: An anti-cancer strategy at the crossroads of glucose and lipid metabolism

“…The sodium salt of a bile acid, sodium deoxycholate 29 (Figure 8), reduced ACLY levels in animal models [110]. Similarly, the polychlorinated biphenyl Aroclor 1254 30 (Figure 8), a toxic compound characterized by a biphenylic scaffold substituted with chlorine atoms, inhibited ACLY (69% inhibition) when administered in the rat diet (0.01% w/v) [111].…”

Screening of Phytochemical Compounds and Toxic Proteinaceous Protease Inhibitor in Some Lesser-Known Food Based Plants and Their Effects and Potential Applications in Food

“…However, the alkaloids and other natural compounds have complex activities and it is necessary to analyze pharmacological activities in the general tissues, linking the structure with the activity presented. It is common to find pharmacological results where a single experimental model generalizes a biological answer, but these can't be accepted because all the pathologies in question are also complex and it is necessary to investigate specific experimental models [3][4][5][6][7][8][9][10][11][12]. This study sets out to investigate of some phytochemical screening of eight Food Plants.…”

Determination of Nutritional Status of Some Vegetables Grown with Different Water Resource Applications in Sargodha, Pakistan

“…Ash values are an evidence of the mineral status, these high values demonstrated that the vegetables are high in mineral concentration. Proof from epidemiological studies suggested that expanded fiber use may add to a lessening in the frequency of specific maladies like diabetes, coronary illness, colon tumor, hypertension, corpulence, and different digestive issue (Eriyamremu and Adamson, 1994). The ash content of present study were in the range 15.79-23.66%, this was significantly higher than that of C. aconitifolius, T. occidentalis and A. cruentus (Kochhar, 1981).…”